Electroactive biomimetic collagen-silver nanowire composite scaffolds

Wickham, Abeni; Vagin, Mikhail; Khalaf, Hazem; Bertazzo, Sérgio; Hodder, Peter; Dånmark, Staffan; Bengtsson, Torbjörn; Altimiras, Jordi; Aili, Daniel

doi:10.1039/c6nr02027e

Cited by 41 publications

(25 citation statements)

References 82 publications

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“…This new class of smart electroactive biomaterials that are able to deliver electrical, electrochemical or electromechanical stimulation directly to cells, tissue, and organs could bring a significant breakthrough in the tissue engineering field [3,5,6]. Electroactive scaffolds have already been investigated for use in neural [4,5,9], muscle [10,11], bone [12][13][14], and cardiac [15,16] tissue engineering applications. A key requirement is the development of biocompatible, biodegradable, and electrically conductive biomaterials.…”

Section: Introductionmentioning

confidence: 99%

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

et al. 2020

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Electrostimulation and electroactive scaffolds can positively influence and guide cellular behaviour and thus has been garnering interest as a key tissue engineering strategy. The development of conducting polymers such as polyaniline enables the fabrication of conductive polymeric composite scaffolds. In this study, we report on the initial development of a polycaprolactone scaffold incorporating different weight loadings of a polyaniline microparticle filler. The scaffolds are fabricated using screw-assisted extrusion-based 3D printing and are characterised for their morphological, mechanical, conductivity, and preliminary biological properties. The conductivity of the polycaprolactone scaffolds increases with the inclusion of polyaniline. The in vitro cytocompatibility of the scaffolds was assessed using human adipose-derived stem cells to determine cell viability and proliferation up to 21 days. A cytotoxicity threshold was reached at 1% wt. polyaniline loading. Scaffolds with 0.1% wt. polyaniline showed suitable compressive strength (6.45 ± 0.16 MPa) and conductivity (2.46 ± 0.65 × 10−4 S/cm) for bone tissue engineering applications and demonstrated the highest cell viability at day 1 (88%) with cytocompatibility for up to 21 days in cell culture.

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Section: Introductionmentioning

confidence: 99%

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

et al. 2020

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confidence: 99%

“…• Silver-Silver (Ag) is a well-known material already used in coatings for biomedical devices and in wound care products thanks to its antibacterial properties. Since it is a metal, it shows electrical conductivity and charge storage capacity [97,98]. It must be underlined that this metal is under debate regarding the aspect of biocompatibility: Ag antibacterial properties are associated to the release of Ag + ions and their release rate can be affected by silver nanoparticles (AgNPs) surface to volume ratio and capping agents, thus leading in toxicity problems [98].…”

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A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

et al. 2019

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During the last years, scientific research in biotechnology has been reporting a considerable boost forward due to many advances marked in different technological areas. Researchers working in the field of regenerative medicine, mechanobiology and pharmacology have been constantly looking for non-invasive methods able to track tissue development, monitor biological processes and check effectiveness in treatments. The possibility to control cell cultures and quantify their products represents indeed one of the most promising and exciting hurdles. In this perspective, the use of conductive materials able to map cell activity in a three-dimensional environment represents the most interesting approach. The greatest potential of this strategy relies on the possibility to correlate measurable changes in electrical parameters with specific cell cycle events, without affecting their maturation process and considering a physiological-like setting. Up to now, several conductive materials has been identified and validated as possible solutions in scaffold development, but still few works have stressed the possibility to use conductive scaffolds for non-invasive electrical cell monitoring. In this picture, the main objective of this review was to define the state-of-the-art concerning conductive biomaterials to provide researchers with practical guidelines for developing specific applications addressing cell growth and differentiation monitoring. Therefore, a comprehensive review of all the available conductive biomaterials (polymers, carbon-based, and metals) was given in terms of their main electric characteristics and range of applications.

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“…AgNPs have been used as antimicrobial agents in wound dressing [6], coating of catheters [7] and of cardiovascular implants [8], bone cements [9] and dental materials [10]. Currently literature on biomedical applications of AgNWs is still limited [2]; emerging applications include textiles [11], wound coatings [12], drug release and tissue regeneration [13].In this work, we explore for the first time the potential use of AgNWs in composite materials designed to prevent infection in bone regeneration applications, as they can provide advantages such as biocompatibility, bioactivity and a 3D structure that can be employed as supporting scaffold. Bone defects remain a major problem in orthopedics and due to the deficiencies of current commercially available bone grafts and alternative materials are needed [14].…”

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confidence: 99%

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confidence: 99%

Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

Mori¹,

Hafidh²,

Mele³

et al. 2019

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One-dimensional nanostructures such as silver nanowires (AgNWs) have attracted considerable attention owing to their outstanding electrical, thermal and antimicrobial properties; however, their application in the prevention of infections linked to bone tissue regeneration interventions has not yet been explored. Here we report on the development of an innovative scaffold prepared from chitosan, composite hydroxyapatite and AgNWs (CS-HACS-AgNWs) having both bioactive and antibacterial properties. In vitro results highlighted the antibacterial potential of AgNWs against both gram-positive and gram-negative bacteria. The CS-HACS-AgNWs composite scaffold demonstrated suitable Ca/P deposition, improved gel strength, reduced gelation time, and sustained Ag + release within therapeutic concentrations. Antibacterial studies showed that the composite formulation was capable of inhibiting bacterial growth in suspension and of completely preventing biofilm formation on the scaffold in the presence of resistant strains. The hydrogels were also shown to be biocompatible, allowing cell proliferation. In summary, the developed CS-HACS-AgNWs composite hydrogels demonstrated significant potential as a scaffold material to be employed in bone regenerative medicine, as they present enhanced mechanical strength combined with the ability to allow calcium salts deposition, while efficiently decreasing the risk of infections. The results presented justify further investigations into potential clinical applications of these materials.to the bulk materials due to their reduced dimensions, controlled structure and large surface-tovolume ratio [3].One, two, and three-dimensional silver nanostructures have been previously reported, with many studies focusing on silver nanowires (AgNWs). AgNWs are anisotropically grown to obtain an aspect ratio higher than 10 and are typically 10-200 nm in diameter and 5-100 µm in length [2]. To date, silver nanoparticles (AgNPs) have been much more extensively studied than AgNWs, and their distinctive high thermal and electrical conductivity, surface-enhanced Raman scattering, chemical stability, catalytic activity and non-linear optical behavior have been highlighted; as well as their antibacterial activity that has extended their usage into several biomedical fields [4]. They are promising agents against a wide range of fungi, viruses and bacterial species; even though the mechanism of action is still not entirely understood, it is believed that nanosilver serves as a source for Ag + ions that can rupture microbial cell walls, denature proteins, block cell respiration and eventually induce cell death [5]. AgNPs have been used as antimicrobial agents in wound dressing [6], coating of catheters [7] and of cardiovascular implants [8], bone cements [9] and dental materials [10]. Currently literature on biomedical applications of AgNWs is still limited [2]; emerging applications include textiles [11], wound coatings [12], drug release and tissue regeneration [13].In this work, we explore for the first time the...

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Electroactive biomimetic collagen-silver nanowire composite scaffolds

Cited by 41 publications

References 82 publications

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

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