Electroactive dendrimer-encapsulated silver nanoparticles for sensing low-abundance proteins with signal amplification

Pei, Xiaomei; Xu, Zonghui; Zhang, Jiayu; Liu, Zhe; Tian, Jinnian

doi:10.1039/c3ay40518d

Cited by 17 publications

(10 citation statements)

References 42 publications

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“…In particular, dendrimers with encapsulated metallic nanoparticles (mostly Au and Pt) display the advantages of both nanocomponents including high density of active groups for biomolecules immobilization, excellent structural homogeneity, remarkable conductivity, catalytic ability and good biocompatibility [ 26 ]. These hybrid nanomaterials have demonstrated to be particularly attractive options to be exploited in electrochemical affinity biosensing as both electrode modifiers [ 26 , 27 , 28 ] and efficient nanocarriers [ 29 , 30 , 31 ].…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

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Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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“…Several nanohybrids of PAMAM and metal nanoparticles have demonstrated to be useful to design original signal amplification strategies for electrochemical immunosensors. As an example, Pei and co-workers developed a novel enzyme-free electrochemical immunoassay for PSA by using a PAMAM G-6.0 dendrimer loaded with Ag nanoparticles as a molecular tag for an anti-PSA polyclonal antibody [67]. This adduct was employed as a detection and signal amplification element in a sandwich-type immunoassay assembled on carbon screen-printed electrodes functionalized with capture anti-PSA antibodies (Figure 13).…”

Section: Dendrimers For Signaling and Signal Amplification In Electromentioning

confidence: 99%

“…Schematic representation of the preparation of the Ag nanoparticles (blue circles)-PAMAM dendrimer nanohybrid and assembly of the immunosensor for PSA. Adapted with permission from Ref [67],. copyright The Royal Society of Chemistry, 2013.…”

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confidence: 99%

Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors

et al. 2019

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Electrochemical immunosensors are antibody-based affinity biosensors with a high impact on clinical, environmental, food, and pharmaceutical analysis. In general, the analytical performance of these devices is critically determined by the materials and reagents used for their construction, signal production and amplification. Dendrimers are monodisperse and highly branched polymers with three-dimensional structures widely employed as “soft” nanomaterials in electrochemical immunosensor technology. This review provides an overview on the state-of-the-art in dendrimer-based electrochemical immunosensors, focusing on those using polyamidoamine and poly (propylene imine) dendrimers. Special emphasis is given to the most original methods recently reported for the construction of immunosensor architectures incorporating dendrimers, as well as to novel sensing approaches based on dendrimer-assisted signal enhancement strategies.

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“…More recently, Pei et al. developed a immunosensor for fPSA based on a sandwich‐type immunoassay using SPEs nanostructured with AuNPs as transducer and a signal amplification by electroactive silver‐mediated poly(amidoamine) dendrimer nanostructures for detection. In this case the assay takes 25 min and enzymes are not necessary since the silver nanoparticles can directly catalyse the reduction of H 2 O 2 without the participation of bioactive enzymes.…”

Section: Cancer Biomarkers Detectionmentioning

confidence: 99%

Screen‐printed Electrochemical Immunosensors for the Detection of Cancer and Cardiovascular Biomarkers

Costa‐Rama

Costa-Garcı́a

2016

Electroanalysis

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1Introduction during the last years,S PEs have gone through significant improvements related to both their design and printing materials.S ince cancera nd cardiovascular diseases are the majort hreats of global health, there is ag rowing demand for the development of portable,r apid, simple and inexpensive devices for the detectiono ft hese diseases.T his article presents an overviewa bout the main biomarkers of cancer and cardiovascular diseases and the EIs based on SPEs for the detection of these biomarkers.

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Electroactive dendrimer-encapsulated silver nanoparticles for sensing low-abundance proteins with signal amplification

Cited by 17 publications

References 42 publications

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors

Screen‐printed Electrochemical Immunosensors for the Detection of Cancer and Cardiovascular Biomarkers

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