Electroactive Smart Materials: Novel Tools for Tailoring Bacteria Behavior and Fight Antimicrobial Resistance

Fernandes, Margarida M.; Carvalho, Estela; Lanceros‐Méndez, S.

doi:10.3389/fbioe.2019.00277

Cited by 23 publications

(38 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This material was selected due to its magnetoelectrical properties, i.e., actively responding to the magnetic field provided by the magnetic bioreactor. Due to their magnetostrictive component (TD), the material senses the magnetic field, inducing a mechanical stimulation on PVDF, which due to its piezoelectric properties further induce an electrical polarization variation, creating the electrically active microenvironment that is translated to the cells [46].…”

Section: Bioreactor Evaluationmentioning

confidence: 99%

Magnetic Bioreactor for Magneto-, Mechano- and Electroactive Tissue Engineering Strategies

Castro

Fernandes

Ribeiro

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically stimulated by a bioreactor, providing the stimuli to the cells, allows a biomimetic approach and thus, mimicking the required microenvironment for effective growth and differentiation of bone cells. In this work, a bioreactor has been designed and built allowing to magnetically stimulate magnetoelectric scaffolds and therefore provide mechanical and electrical stimuli to the cells through magnetomechanical or magnetoelectrical effects, depending on the piezoelectric nature of the scaffold. While mechanical bioreactors need direct application of the stimuli on the scaffolds, the herein proposed magnetic bioreactors allow for a remote stimulation without direct contact with the material. Thus, the stimuli application (23 mT at a frequency of 0.3 Hz) to cells seeded on the magnetoelectric, leads to an increase in cell viability of almost 30% with respect to cell culture under static conditions. This could be valuable to mimic what occurs in the human body and for application in immobilized patients. Thus, special emphasis has been placed on the control, design and modeling parameters governing the bioreactor as well as its functional mechanism.

show abstract

Section: Bioreactor Evaluationmentioning

confidence: 99%

Magnetic Bioreactor for Magneto-, Mechano- and Electroactive Tissue Engineering Strategies

Castro

Fernandes

Ribeiro

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…Graphene and hBN have been recently found to induce specific cellular responses in different contexts. Taking into consideration the issues related to bacterial infection and the relevance of degenerative diseases, the different biological interactions of these materials can be exploited toward the following aims: (1) inducing bacterial cell death, e.g., to design medical instruments and mitigate the spreading of infections in hospital environment, and to produce anti-biofouling coatings ( Parra et al, 2015c , 2017 ; Zurob et al, 2019 ); (2) stimulating cellular growth, such as in the case of tissue engineering, wound healing, and bone tissue regeneration ( Fernandes et al, 2019 ; Şen et al, 2019 ; Aki et al, 2020 ). Devices such as epidermal electronics, intra-cortical implants for sensing and brain tissue regeneration require intimate contact with biological systems.…”

Section: Introductionmentioning

confidence: 99%

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

Santos

Moschetta

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Two-dimensional material (2DM) coatings exhibit complex and controversial interactions with biological matter, having shown in different contexts to induce bacterial cell death and contribute to mammalian cell growth and proliferation in vitro and tissue differentiation in vivo. Although several reports indicate that the morphologic and electronic properties of the coating, as well as its surface features (e.g., crystallinity, wettability, and chemistry), play a key role in the biological interaction, these kinds of interactions have not been fully understood yet. In this review, we report and classify the cellular interaction mechanisms observed in graphene and hexagonal boron nitride (hBN) coatings. Graphene and hBN were chosen as study materials to gauge the effect of two atomic-thick coatings with analogous lattice structure yet dissimilar electrical properties upon contact with living matter, allowing to discern among the observed effects and link them to specific material properties. In our analysis, we also considered the influence of crystallinity and surface roughness, detailing the mechanisms of interaction that make specific coatings of these 2DMs either hostile toward bacterial cells or innocuous for mammalian cells. In doing this, we discriminate among the material and surface properties, which are often strictly connected to the 2DM production technique, coating deposition and post-processing method. Building on this knowledge, the selection of 2DM coatings based on their specific characteristics will allow to engineer desired functionalities and devices. Antibacterial coatings to prevent biofouling, biocompatible platforms suitable for biomedical applications (e.g., wound healing, tissue repairing and regeneration, and novel biosensing devices) could be realized in the next future. Overall, a clear understanding on how the 2DM coating’s properties may modulate a specific bacterial or cellular response is crucial for any future innovation in the field.

show abstract

“…Overuse and misuse of antibiotics has led to a dramatic increase of bacteria resistant to antibiotics 1 , 2 . Serious infections (bacteremia, pneumonia, complicated skin infection, etc .)…”

Section: Introductionmentioning

confidence: 99%

“…The dysregulated host inflammatory reaction to systemic infection (sepsis) can further cause life-threatening multiple-organ dysfunction 4 - 6 . Antibiotic-resistant bacteria are killing 750 000 people every year 1 . If no effective action is taken, the antibiotic-resistant infection-associated deaths are predicted to reach more than 10 million per year by 2050 1 .…”

Section: Introductionmentioning

confidence: 99%

Fructose-coated Ångstrom silver prevents sepsis by killing bacteria and attenuating bacterial toxin-induced injuries

Yin¹,

Zhou²,

Chen³

et al. 2021

Theranostics

View full text Add to dashboard Cite

Serious infection caused by multi-drug-resistant bacteria is a major threat to human health. Bacteria can invade the host tissue and produce various toxins to damage or kill host cells, which may induce life-threatening sepsis. Here, we aimed to explore whether fructose-coated Ångstrom-scale silver particles (F-AgÅPs), which were prepared by our self-developed evaporation-condensation system and optimized coating approach, could kill bacteria and sequester bacterial toxins to attenuate fatal bacterial infections. Methods: A series of in vitro assays were conducted to test the anti-bacterial efficacy of F-AgÅPs, and to investigate whether F-AgÅPs could protect against multi-drug resistant Staphylococcus aureus ( S. aureus )- and Escherichia coli ( E. coli )-induced cell death, and suppress their toxins ( S. aureus hemolysin and E. coli lipopolysaccharide)-induced cell injury or inflammation. The mouse models of cecal ligation and puncture (CLP)- or E. coli bloodstream infection-induced lethal sepsis were established to assess whether the intravenous administration of F-AgÅPs could decrease bacterial burden, inhibit inflammation, and improve the survival rates of mice. The levels of silver in urine and feces of mice were examined to evaluate the excretion of F-AgÅPs. Results: F-AgÅPs efficiently killed various bacteria that can cause lethal infections and also competed with host cells to bind with S. aureus α-hemolysin, thus blocking its cytotoxic activity. F-AgÅPs inhibited E. coli lipopolysaccharide-induced endothelial injury and macrophage inflammation, but not by directly binding to lipopolysaccharide. F-AgÅPs potently reduced bacterial burden, reversed dysregulated inflammation, and enhanced survival in mice with CLP- or E. coli bloodstream infection-induced sepsis, either alone or combined with antibiotic therapy. After three times injections within 48 h, 79.18% of F-AgÅPs were excreted via feces at the end of the 14-day observation period. Conclusion: This study suggests the prospect of F-AgÅPs as a promising intravenous agent for treating severe bacterial infections.

show abstract

Electroactive Smart Materials: Novel Tools for Tailoring Bacteria Behavior and Fight Antimicrobial Resistance

Cited by 23 publications

References 85 publications

Magnetic Bioreactor for Magneto-, Mechano- and Electroactive Tissue Engineering Strategies

Magnetic Bioreactor for Magneto-, Mechano- and Electroactive Tissue Engineering Strategies

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

Fructose-coated Ångstrom silver prevents sepsis by killing bacteria and attenuating bacterial toxin-induced injuries

Contact Info

Product

Resources

About