Electroacupuncture Alleviates Experimental Chronic Inflammatory Pain by Inhibiting Calcium Voltage‐Gated Channel‐Mediated Inflammation

Zhou, Jie; Ying, Jiang; Ma, Ruijie; Song, Hongyun; Chen, Qin; Chai, Yueyang; Liang, Yi; Zhou, You; Fang, Jianqiao

doi:10.1155/2020/7061972

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…In this study, GSEA results showed that CHRNA3 was enriched in neuroactive ligand-receptor interaction, ECM-receptor interaction, and IL-17 signaling pathway-related pathways, while CHRNB2 and CHRNB4 were related to ferroptosis, the toll-like receptor signaling pathway, the hedgehog signaling pathway, cell cycle, the mTOR signaling pathway, and inflammatory mediator regulation of TRP channels, respectively. Most of these signaling pathways mediate nerve injury and the continuation of NPP by participating in neurotoxicity [ 31 ], neural activity [ 32 ], inflammatory response [ 33 , 34 ], hyperalgesia [ 35 ], nerve healing [ 36 , 37 ], microglia polarization [ 38 ], and oxidative stress [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, GSEA results showed that CHRNA3 was enriched in neuroactive ligandreceptor interaction, ECM-receptor interaction, and IL-17 signaling pathway-related pathways, while CHRNB2 and CHRNB4 were related to ferroptosis, the toll-like receptor signaling pathway, the hedgehog signaling pathway, cell cycle, the mTOR signaling pathway, and inflammatory mediator regulation of TRP channels, respectively. Most of these signaling pathways mediate nerve injury and the continuation of NPP by participating in neurotoxicity [31], neural activity [32], inflammatory response [33,34], hyperalgesia [35], nerve healing [36,37], microglia polarization [38], and oxidative stress [39]. e nicotine and nicotinic acetylcholine receptors (NAChRs) subtype, by a variety of subunits of combinatorial synthesis channel receptor complexes, its structure, and function in the nervous system of diversity, had been found to enhance the presynaptic neurotransmitter and release and affect the excitability of neurons, and when its function declines, it would cause the nervous system dysfunction [40,41].…”

Section: Discussionmentioning

confidence: 99%

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Application of Bioinformatics Methods to Identify Key Genes and Functions in Chronic Pelvic Pain

Sun

2021

Evidence-Based Complementary and Alternative Medicine

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Neuropathologic pain (NPP) occurs in most patients with chronic pelvic pain (CPP), and the unique physiological characteristics of visceral sensory neurons make the current analgesic effect of CPP patients not optimistic. Therefore, this study explored the possible biological characteristics of key genes in CPP through the bioinformatics method. CPP-related dataset GSE131619 was downloaded from Gene Expression Omnibus to investigate the differentially expressed genes (DEGs) between lumbar dorsal root ganglia (DRG) and sacral DRG, and the functional enrichment analysis was performed. A protein-protein interaction (PPI) network was constructed to search subnet modules of specific biological processes, and then, the genes in the subnet were enriched by single gene set analysis. A CPP mouse model was established, and the expression of key genes were identified by qPCR. The results showed that 127 upregulated DEGs and 103 downregulated DEGs are identified. Functional enrichment analysis showed that most of the genes involved in signal transduction were involved in the pathway of receptor interaction. A subnet module related to neural signal regulation was identified in PPI, including CHRNB4, CHRNA3, and CHRNB2. All three genes were associated with neurological or inflammatory activity and are downregulated in the sacral spinal cord of CPP mice. This study provided three key candidate genes for CPP: CHRNB4, CHRNA3, and CHRNB2, which may be involved in the occurrence and development of CPP, and provided a powerful molecular target for the clinical diagnosis and treatment of CPP.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of Bioinformatics Methods to Identify Key Genes and Functions in Chronic Pelvic Pain

Sun

2021

Evidence-Based Complementary and Alternative Medicine

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“…Notably, myalgias, headache and abdominal pain are all symptoms of COVID-19 [ 16 ], which can be regulated by calcium signaling pathway and neuroactive ligand–receptor interaction [ 17 ]. A study using RNA-sequencing found that experimental chronic inflammatory pain could be relieved by electroacupuncture via the suppression of calcium voltage-gated channel-mediated inflammation [ 90 ]. Although most calcium signaling pathway studies focused on pain and cardiovascular disease research, it has also been shown to be associated with inflammatory responses [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Is acupuncture effective in the treatment of COVID-19 related symptoms? Based on bioinformatics/network topology strategy

Han

Zhang

Wang

et al. 2021

Briefings in Bioinformatics

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Acupuncture is an important part of Chinese medicine that has been widely used in the treatment of inflammatory diseases. During the coronavirus disease 2019 (COVID-19) epidemic, acupuncture has been used as a complementary treatment for COVID-19 in China. However, the underlying mechanism of acupuncture treatment of COVID-19 remains unclear. Based on bioinformatics/topology, this paper systematically revealed the multi-target mechanisms of acupuncture therapy for COVID-19 through text mining, bioinformatics, network topology, etc. Two active compounds produced after acupuncture and 180 protein targets were identified. A total of 522 Gene Ontology terms related to acupuncture for COVID-19 were identified, and 61 pathways were screened based on the Kyoto Encyclopedia of Genes and Genomes. Our findings suggested that acupuncture treatment of COVID-19 was associated with suppression of inflammatory stress, improving immunity and regulating nervous system function, including activation of neuroactive ligand–receptor interaction, calcium signaling pathway, cancer pathway, viral carcinogenesis, Staphylococcus aureus infection, etc. The study also found that acupuncture may have additional benefits for COVID-19 patients with cancer, cardiovascular disease and obesity. Our study revealed for the first time the multiple synergistic mechanisms of acupuncture on COVID-19. Acupuncture may play an active role in the treatment of COVID-19 and deserves further promotion and application. These results may help to solve this pressing problem currently facing the world.

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“…Glutamatergic synaptic transmission is coupled with excess Ca 2+ entry into projection neurons and results in the activation of the Ca 2+ -dependent enzyme Ca 2+ /calmodulin-dependent protein kinase II (CamKII) and phosphorylation of the NR2B subunit of NMDAR at postsynaptic sites in the ACC, thus modulating visceral pain in a viscerally hypersensitive model [ 80 ]. It was reported that EA at ST36 and Kunlun (BL60) could reverse the actions of the calcium voltage-gated channel subunit and calcium voltage-gated channel auxiliary subunit γ , thus reducing chronic inflammatory pain (CIP) in the CFA rats [ 47 ].…”

Section: Central Sensitization In Pain Transmission and Modulationmentioning

confidence: 99%

The Role of Neuroglial Crosstalk and Synaptic Plasticity-Mediated Central Sensitization in Acupuncture Analgesia

et al. 2021

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Although pain is regarded as a global public health priority, analgesic therapy remains a significant challenge. Pain is a hypersensitivity state caused by peripheral and central sensitization, with the latter considered the culprit for chronic pain. This study summarizes the pathogenesis of central sensitization from the perspective of neuroglial crosstalk and synaptic plasticity and underlines the related analgesic mechanisms of acupuncture. Central sensitization is modulated by the neurotransmitters and neuropeptides involved in the ascending excitatory pathway and the descending pain modulatory system. Acupuncture analgesia is associated with downregulating glutamate in the ascending excitatory pathway and upregulating opioids, 𝛾-aminobutyric acid, norepinephrine, and 5-hydroxytryptamine in the descending pain modulatory system. Furthermore, it is increasingly appreciated that neurotransmitters, cytokines, and chemokines are implicated in neuroglial crosstalk and associated plasticity, thus contributing to central sensitization. Acupuncture produces its analgesic action by inhibiting cytokines, such as interleukin-1β, interleukin-6, and tumor necrosis factor-α, and upregulating interleukin-10, as well as modulating chemokines and their receptors such as CX3CL1/CX3CR1, CXCL12/CXCR4, CCL2/CCR2, and CXCL1/CXCR2. These factors are regulated by acupuncture through the activation of multiple signaling pathways, including mitogen-activated protein kinase signaling (e.g., the p38, extracellular signal-regulated kinases, and c-Jun-N-terminal kinase pathways), which contribute to the activation of nociceptive neurons. However, the responses of chemokines to acupuncture vary among the types of pain models, acupuncture methods, and stimulation parameters. Thus, the exact mechanisms require future clarification. Taken together, inhibition of central sensitization modulated by neuroglial plasticity is central in acupuncture analgesia, providing a novel insight for the clinical application of acupuncture analgesia.

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Electroacupuncture Alleviates Experimental Chronic Inflammatory Pain by Inhibiting Calcium Voltage‐Gated Channel‐Mediated Inflammation

Cited by 8 publications

References 33 publications

Application of Bioinformatics Methods to Identify Key Genes and Functions in Chronic Pelvic Pain

Application of Bioinformatics Methods to Identify Key Genes and Functions in Chronic Pelvic Pain

Is acupuncture effective in the treatment of COVID-19 related symptoms? Based on bioinformatics/network topology strategy

The Role of Neuroglial Crosstalk and Synaptic Plasticity-Mediated Central Sensitization in Acupuncture Analgesia

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