“…Approximately 15 μg of protein was loaded into NuPAGE 4–12% Bis-Tris protein gels (ThermoFisher, cat# NP0321BOX), separated with 200 V for approximately 1 h, and electrotransferred to PVDF membrane in the XCell II Blot Module for wet (tank) transfer (ThermoFisher) for 90 min at 30 V. The membrane was blocked in 5% non-fat dry milk in TBS buffer, pH 7.4 (Quality Biological, cat# 351-086-101) plus 0.1% Tween 20 (Sigma-Aldrich) for 1 h at room temperature and then exposed to primary antibodies. Antibodies for detection of NEUROD1, CALB1, and CAMKIV were purchased from Cell Signaling: NeuroD (D90G12) rabbit mAb: cat# 7019, 1:1,000 dilution (for WB validation, see https://www.cellsignal.com/products/primary-antibodies/neurod-d90g12-rabbit-mab/7019); Calbindin (D1I4Q) XP rabbit mAb: cat# 13176, 1:1,500 dilution (for WB validation and references, see https://www.cellsignal.com/products/primary-antibodies/calbindin-d1i4q-xp-rabbit-mab/13176?_=1487290902164&Ntt=13176&tahead=true) 80–82 ; CaMK IV: rabbit, cat# 4032, 1:1,000 dilution (for WB validation and references, see https://www.cellsignal.com/products/primary-antibodies/camkiv-antibody/4032; Product Citations) 83,84 . Equal loading was verified by western blotting of actin (α-actin: 1:10,000, mouse, Sigma-Aldrich, clone AC-15, cat# A5441; for WB validation see http://www.sigmaaldrich.com/catalog/product/sigma/a5441?lang=en®ion=US).…”