Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

Liu, Yu-pu; Luo, Zheng; Wang, Changjiang; Cai, Haidong; Zhao, Tiantian; Li, Han; Guo, Hai-dong

doi:10.3389/fnins.2020.525144

Cited by 21 publications

(19 citation statements)

References 46 publications

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“…When Schwann cells exposure promoted directed migration, it travels towards the cathode at a mean rate of 7.5 µm/h (Bunn et al, 2019). EA may promote enhanced sciatic nerve function recovery and SC proliferation by down-regulating miR-1b (Liu et al, 2020). At present, there is no de nitive agreement on which of these regimens is more effective.…”

Section: Results Of Nite Element Model 11 the Location Of The Needlesmentioning

confidence: 99%

An ultrasound-guided percutaneous nerve stimulation regimen based on finite element modeling for median nerve rupture

Chu

Song

et al. 2021

Preprint

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Object: In order to promote the functional recovery of median nerve rupture patients, this paper proposes an ultrasound-guided percutaneous nerve stimulation regimen based on finite element modeling. Method: First, according to anatomy feature, a multi-layer human forearm model is constructed. What’s more, taking current density and activate function as optimization indicators, the percutaneous nerve stimulation regimen is designed with finite element modeling, including electrical needle angel, distance and position. Finally, to test the performance of designed regimen, a total of 22 patients with median nerve rupture participate in the clinical randomized controlled trial. And the clinical treatment effect is evaluated with BMRC, grip strength, functional score of median nerve and DASH scores. Results: The designed percutaneous nerve stimulation regimen is that, parallel to each other, electrical needles are located at both ends of the injury nerve with a distance of 3 cm. Besides, clinical trial results show that, after treatment the difference in sensory function was statistically significant. In terms of motor function, the BMRC of motor and grip strength are improved in both groups after treatment and there are significant differences between groups. Besides the grip strength improvement of experimental group is 4 times higher than that of control group. On global function, the DASH scores are reduced by 29% with experimental group but only 8% with control group; functional score of median nerve improved by 24% in the experimental group and by 22% in the control group, but the difference between groups is statistically significant after treatment. Conclusion: Clinical trial results demonstrate that the designed percutaneous nerve stimulation regimen can significantly improve the sensory, motor and global function of median nerve rupture, which is a potential clinical treatment regimen. Trial registration: ChiCTR, ChiCTR2000030790. Registered14 march 2020, http://www.chictr.org.cn/ChiCTR2000030790

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Section: Results Of Nite Element Model 11 the Location Of The Needlesmentioning

confidence: 99%

An ultrasound-guided percutaneous nerve stimulation regimen based on finite element modeling for median nerve rupture

Chu

Song

et al. 2021

Preprint

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“…For nerve conduction velocity recovery rate (NCV), the NCV of the bilateral sciatic nerve was assessed, as previously described [ 15 ]. In brief, the stimulating electrode and the receiving electrode were connected to the proximal and distal ends of the sciatic nerve, respectively, using the copper handle of the acupuncture needle.…”

Section: Methodsmentioning

confidence: 99%

“…Electroacupuncture (EA) is defined as a combination of electrical stimulation with acupuncture needles and is capable of promoting the regeneration of the transected median nerve in rats [ 13 ] and increasing neuronal stem cell growth [ 14 ]. We have confirmed that EA could promote Schwann cells (SC) proliferation and the release of neurotrophic factors (NTFs), including brain-derived neurotrophic factor (BDNF) [ 15 ] and nerve growth factor (NGF) [ 16 ] after PNI.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we aimed to elucidate new mechanisms of EA treatment for PNI via the regulation of miRNAs. It has been proven that the expressions of many different miRNAs are changed after PNI [ 15 , 31 – 34 ], indicating its association with the occurrence and development of the nervous system [ 35 , 36 ]. miRNA-21 (miR-21) expression was upregulated following PNI in the serum [ 37 ], local injury tissue [ 38 ], and dorsal root ganglion (DRG) [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

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Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Liu

Yang

Mou

et al. 2022

Oxidative Medicine and Cellular Longevity

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Purpose. Our study is aimed at investigating the mechanism by which electroacupuncture (EA) promoted nerve regeneration by regulating the release of exosomes and exosome-mediated miRNA-21 (miR-21) transmission. Furthermore, the effects of Schwann cells- (SC-) derived exosomes on the overexpression of miR-21 for the treatment of PNI were investigated. Methods. A sciatic nerve injury model of rat was constructed, and the expression of miR-21 in serum exosomes and damaged local nerves was detected using RT-qPCR after EA treatment. The exosomes were identified under a transmission electron microscope and using western blotting analysis. Then, the exosome release inhibitor, GW4869, and the miR-21-5p-sponge used for the knockdown of miR-21 were used to clarify the effects of exosomal miR-21 on nerve regeneration promoted by EA. The nerve conduction velocity recovery rate, sciatic nerve function index, and wet weight ratio of gastrocnemius muscle were determined to evaluate sciatic nerve function recovery. SC proliferation and the level of neurotrophic factors were assessed using immunofluorescence staining, and the expression levels of SPRY2 and miR-21 were detected using RT-qPCR analysis. Subsequently, the transmission of exosomal miR-21 from SC to the axon was verified in vitro. Finally, the exosomes derived from the SC infected with the miR-21 overexpression lentivirus were collected and used to treat the rat SNI model to explore the therapeutic role of SC-derived exosomes overexpressing miR-21. Results. We found that EA inhibited the release of serum exosomal miR-21 in a PNI model of rats during the early stage of PNI, while it promoted its release during later stages. EA enhanced the accumulation of miR-21 in the injured nerve and effectively promoted the recovery of nerve function after PNI. The treatment effect of EA was attenuated when the release of circulating exosomes was inhibited or when miR-21 was downregulated in local injury tissue via the miR-21-5p-sponge. Normal exosomes secreted by SC exhibited the ability to promote the recovery of nerve function, while the overexpression of miR-21 enhanced the effects of the exosomes. In addition, exosomal miR-21 secreted by SC could promote neurite outgrowth in vitro. Conclusion. Our results demonstrated the mechanism of EA on PNI from the perspective of exosome-mediated miR-21 transport and provided a theoretical basis for the use of exosomal miR-21 as a novel strategy for the treatment of PNI.

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“…In vitro , it was confirmed that the overexpression of mir-1b could inhibit the expression of brain-derived neurotrophic factor (BDNF) in the rat Schwann cell line. The research may indicate that the electroacupuncture might promote Schwann cell proliferation and recovery of sciatic nerve function by downregulating mir-1b ( 26 ).…”

Section: Recovery Of Function After Traumamentioning

confidence: 99%

Tissue Engineering Strategies for Peripheral Nerve Regeneration

Yin

Ren

et al. 2021

Front. Neurol.

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A peripheral nerve injury (PNI) has severe and profound effects on the life of a patient. The therapeutic approach remains one of the most challenging clinical problems. In recent years, many constructive nerve regeneration schemes are proposed at home and abroad. Nerve tissue engineering plays an important role. It develops an ideal nerve substitute called artificial nerve. Given the complexity of nerve regeneration, this review summarizes the pathophysiology and tissue-engineered repairing strategies of the PNI. Moreover, we discussed the scaffolds and seed cells for neural tissue engineering. Furthermore, we have emphasized the role of 3D printing in tissue engineering.

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Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

Cited by 21 publications

References 46 publications

An ultrasound-guided percutaneous nerve stimulation regimen based on finite element modeling for median nerve rupture

An ultrasound-guided percutaneous nerve stimulation regimen based on finite element modeling for median nerve rupture

Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Tissue Engineering Strategies for Peripheral Nerve Regeneration

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