Electroacupuncture Reduces Inflammation but Not Bone Loss on Periodontitis in Arthritic Rats

Gusmão, Jonas Nogueira Ferreira Maciel; Fonseca, Khetyma Moreira; Ferreira, Bruno Sousa Pinto; Alves, Bruno Wesley de Freitas; Júnior, Howard Lopes Ribeiro; Lisboa, Mário Roberto Pontes; Pereira, Karuza Maria Alves; Vale, Mariana Lima; Gondim, Delane Viana

doi:10.1007/s10753-020-01313-x

Cited by 9 publications

(12 citation statements)

References 34 publications

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“…The regulation of cytokines is one of the basic functions of EA, especially by maintaining the balance between different immune cells (Li et al, 2019). We found that EA can reduce the expression of pro‐inflammatory factors and increase the expression of anti‐inflammatory factors in the periodontal tissues of LIP mice, which is similar to the previous results in the rat model (Lisboa et al, 2015; Alves et al, 2019; Gusmão et al, 2021). Moreover, in an Alzheimer's disease model, Xie et al (2021) found that EA improved the effect of brain lesions in rats by promoting the polarization of microglia towards M2‐type cells and decreasing the activation of M1‐type cells, which is consistent with the trend of Arg‐1/iNOS in the present study.…”

Section: Discussionsupporting

confidence: 91%

“…This study confirmed the therapeutic effect of EA on periodontal bone resorption and extended previous findings (Lisboa et al, 2015; Alves et al, 2019; Ribeiro et al, 2020). However, Gusmão et al found that EA only resulted in a reduction of NF‐κB, but did not ameliorate the alveolar bone loss in arthritic rats with experimental periodontitis, which may be explained by the fact that periodontitis was worsened by the presence of severe arthritis in their model (Gusmão et al, 2021). Notably, in all these studies, no removal of periodontal irritants was performed, which may also be the reason why EA did not completely prevent bone resorption.…”

Section: Discussionmentioning

confidence: 97%

“…Not only does EA regulate immunity and microbial composition and function in many diseases at the same time, including intestinal obstruction, sepsis, and colitis (Torres‐Rosas et al, 2014; Y. Xiao et al, 2020; N. N. Yang, Yang, et al, 2021), but it is also able to exert systemic anti‐inflammatory effects through the neuro–endocrine–immune pathway (Liu et al, 2021). In an experimental periodontitis rat model, EA could control periodontal tissue breakdown and reduce the expression of some pro‐inflammatory and pro‐resorptive factors, and remarkably increase the expression of the immunomodulatory agent CB1 in neural tissues and CB2 in periodontal tissues (Lisboa et al, 2015; Alves et al, 2019; Gusmão et al, 2021). Thus, EA could serve as a potential adjunctive technique to help control periodontal inflammation and bone resorption.…”

Section: Introductionmentioning

confidence: 99%

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Electroacupuncture regulates macrophage, neutrophil, and oral microbiota to alleviate alveolar bone loss and inflammation in experimental ligature‐induced periodontitis

Liu

Yang

Wang

et al. 2022

J Clinic Periodontology

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Aim Electroacupuncture (EA) regulates distant body physiology through somatic sensory autonomic reflexes, balances the microbiome, and can promote the release of immune cells into bloodstream, thereby inhibiting severe systemic inflammation. This makes it possible to use EA as an integrated treatment for periodontitis. Materials and Methods In this study, EA was applied to the ST36 acupoints in a ligature‐induced periodontitis (LIP) mouse model. Then the effects of EA on periodontal myeloid cells, cytokines, and the microbiome were comprehensively analysed using flow cytometry, quantitative Polymerase Chain Reaction (PCR), and 16 S sequencing. Results Results demonstrated that EA could significantly relieve periodontal bone resorption. EA also suppressed the infiltration of macrophages and neutrophils, reduced gene expression of the pro‐inflammatory cytokines IL‐1β, IL‐6, IL‐17 and TNF‐α, and increased expression of the anti‐inflammatory factors IL‐4 and IL‐10 in periodontal tissues. Moreover, composition of the periodontal microbiome was regulated by EA, finding that complex of microbiota, including supragingival Veillonella, subgingival Streptococcus, and subgingival Erysipelatoclostridium, were significantly reduced. Meanwhile, nitrate and nitrate‐related activities of subgingival microbiota were reversed. Network analysis revealed close relationships among Veillonella, Streptococcus, and Bacteroides. Conclusions Our study indicates that EA can effectively alleviate inflammation and bone resorption in LIP mice, potentially via the regulation of myeloid cells, cytokines, and periodontal microbiome.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Electroacupuncture regulates macrophage, neutrophil, and oral microbiota to alleviate alveolar bone loss and inflammation in experimental ligature‐induced periodontitis

Liu

Yang

Wang

et al. 2022

J Clinic Periodontology

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“…The quality assessments of the 21 studies are summarized in Figure 2 . 13 studies were rated as having a low risk for the sequence generation bias, as they mentioned the rats were randomly grouped ( Yu et al, 2009 ; He et al, 2011 ; Qiao et al, 2012 ; Shou et al, 2013 ; Zhu et al, 2015 ; Dong et al, 2018 ; Ma et al, 2018 ; Xu et al, 2018 ; Su et al, 2019 ; Zhu et al, 2019 ; Gusmao et al, 2021 ; Shen et al, 2021 ; Yang et al, 2021 ; Zheng et al, 2021 ; Yu et al, 2022 ); the remaining 8 studies did not mention the criteria for grouping. All the studies started with rats of similar weights, and they were kept under similar environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

The effect of acupuncture on pain and swelling of arthritis animal models: A systematic review and meta-analysis

Kim

2023

Front. Genet.

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Arthritis, the inflammation of joints, attributes to the patient’s pain, joint deformation, and limited range of motion. Emerging studies have shown the effects of acupuncture on different types of arthritis. We aimed to assess the effects of acupuncture on arthritis animal models and summarize the related mechanisms. We retrieved studies that met our criteria from PubMed, MEDLINE, EMBASE and the Research Information Service System. The quality assessment was evaluated by using the Systematic Review Centre for Laboratory Animal Experimentation’s risk of bias tool. The pain withdrawal latency, pain withdrawal threshold, and paw volume data were digitized using Engauge Digitizer software. The meta-analysis was performed, and the figures were generated using RevMan software. The meta-analysis of data from 21 animal studies revealed that acupuncture increased tolerance to pain stimuli, and reduced swelling in arthritis animals. Although the number of included studies is insufficient, the results suggest acupuncture to be effective in improving arthritis-induced inflammation and pain by regulating the nervous and immune system.

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“…At present, it is widely accepted that the cytokine network plays a vital role in RA pathogenesis [ 13 ], and anticytokines therapy in patients with RA has proven to be effective [ 14 ]. Moreover, EA has been previously reported to reduce the serum levels of pro-inflammatory cytokines in RA patients [ 15 ] and RA animal models [ 16 , 17 ], which suggests the possible immunomodulatory mechanism of EA in alleviating RA.…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture Alleviates Pain Responses and Inflammation in Collagen‐Induced Arthritis Rats via Suppressing the TLR2/4‐MyD88‐NF‐κB Signaling Pathway

Sun

Yan

Qiao

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Background and Purpose. Electroacupuncture (EA) is effective on rheumatoid arthritis (RA), an autoimmune disease, but the mechanisms involved remain poorly understood. This study was designed to investigate the analgesic and anti-inflammatory effects of EA in a chronic inflammatory animal model of collagen-induced arthritis (CIA) and its underlying molecular mechanisms. Experimental Approach. For the male Sprague–Dawley (SD) rats were immunized with bovine type II collagen followed by a booster injection 7 days later. Two weeks after the first immunization, EA stimulation (2/100 Hz, 1 mA, lasting for 30 min/day) was delivered to Zusanli (ST36), and Sanyinjiao (SP6) or OxPAPC (TLR2/TLR4 inhibitor, 1.5 mg/kg) was injected by tail vein for 28 days. After intervention, the analgesic effect was evaluated from the aspect of pain responses including thermal withdrawal latency (TWL) and mechanical withdrawal thresholds (MWT). The anti-inflammatory effect was assessed by paw edema detection, histopathological analysis, and Meso Scale Discovery (MSD) testing of tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). The underlying molecular mechanism was analyzed through western blotting and double-immunofluorescence labeling. Results. EA intervention and OxPAPC injection could relieve mechanical allodynia and thermal hyperalgesia caused by CIA. Paw edema and pathological damage of synovium were significantly ameliorated after EA intervention and OxPAPC injection. Furthermore, EA intervention and OxPAPC injection markedly reduced the contents of serum TNF-α, IL-1β, and IL-6, as well as the protein expression levels of synovial TLR2, TLR4, MyD88, and NF-κB p-p65. In particular, the expression of TLR2 and TLR4 on synovial fibroblasts and macrophages in synovium was significantly reduced by EA intervention. Conclusions. Repeated EA stimulation at ST36 and SP6 can effectively relieve joint pain and synovial inflammation caused by RA in CIA rats. The analgesic and anti-inflammatory effect of EA may be closely related to the inhibition of innate immune responses driven by the TLR2/4-MyD88-NF-κB signaling pathway in the synovium.

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Electroacupuncture Reduces Inflammation but Not Bone Loss on Periodontitis in Arthritic Rats

Cited by 9 publications

References 34 publications

Electroacupuncture regulates macrophage, neutrophil, and oral microbiota to alleviate alveolar bone loss and inflammation in experimental ligature‐induced periodontitis

Electroacupuncture regulates macrophage, neutrophil, and oral microbiota to alleviate alveolar bone loss and inflammation in experimental ligature‐induced periodontitis

The effect of acupuncture on pain and swelling of arthritis animal models: A systematic review and meta-analysis

Electroacupuncture Alleviates Pain Responses and Inflammation in Collagen‐Induced Arthritis Rats via Suppressing the TLR2/4‐MyD88‐NF‐κB Signaling Pathway

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