Electroanalysis of NADH Using Conducting and Redox Active Polymer/Carbon Nanotubes Modified Electrodes-A Review

Kumar, Sudesh; Chen, Shen‐Ming

doi:10.3390/s8020739

Cited by 135 publications

(71 citation statements)

References 120 publications

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“…The retention can be achieved by mediator immobilization at the conducting surface. This review is focused on the applicability of immobilized mediators for the oxidation of reduced NAD(P)H. For more information about the electrochemical basics and mechanistic investigations, please read the following reviews: Gorton and Domínguez (2002), Katakis and Domínguez (1997), Kumar and Chen (2008), and Radoi and Compagnone (2009).…”

Section: Introductionmentioning

confidence: 99%

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Kochius

Magnusson

Hollmann

et al. 2012

Appl Microbiol Biotechnol

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The applicability of dissolved redox mediators for NAD(P) + regeneration has been demonstrated several times. Nevertheless, the use of mediators in solutions for sensor applications is not a very convenient strategy since the analysis is not reagentless and long stabilization times occur. The most important drawbacks of dissolved mediators in biocatalytic applications are interferences during product purification, limited reusability of the mediators, and their cost-intensive elimination from wastewater. Therefore, the use of immobilized mediators has both economic and ecological advantages. This work critically reviews the current stateof-art of immobilized redox mediators for electrochemical NAD(P) + regeneration. Various surface modification techniques, such as adsorption polymerization and covalent linkage, as well as the corresponding NAD(P) + regeneration rates and the operational stability of the immobilized mediator films, will be discussed. By comparison with other existing regeneration systems, the technical potential and future perspectives of biocatalytic redox reactions based on electrochemically fed immobilized mediators will be assessed.

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Section: Introductionmentioning

confidence: 99%

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Kochius

Magnusson

Hollmann

et al. 2012

Appl Microbiol Biotechnol

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“…Some mediators (electrocatalysts) were immobilized on the electrode surface by covalent attachment, electrochemical polymerization, incorporation in carbon paste, adsorption, self-assembly and via entrapment in polymeric matrices [4,[17][18][19]. Another strategy is modification of the electrode surface with a polymeric substance, using electrodes modified with carbon nanotubes, nanofibers or using enzymatic methods that follow bioelectrocatalytic reaction [2,4,13,15,16,[20][21][22][23]. Investigation in this area usually explores the mediator use or the surface modification to improve the electrochemical detection [15,21,[24][25][26][27][28].…”

Section: David Publishingmentioning

confidence: 99%

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

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Abstract:A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 µL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD + redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD + signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD + /NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).

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“…In recent years, electrochemically synthesized polymer films on electrode surface considered for potential applications in electroanalysis of biological molecules [9,44]. Electropolymerization is a good approach to prepare polymer modified electrodes (PMEs) as adjusting electrochemical parameters can control film thickness, permeation and charge transport characteristics.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Sensor for Detection of the Reduced Form of Nicotinamide Adenine Dinucleotide Using a Poly(pyronin B) Film Modified Electrode

Kumar

Chen

2009

Electroanalysis

Self Cite

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An electrochemical method for the preparation of poly(pyronin B) film was proposed in this paper. A poly(pyronin B) (poly(PyB)) film modified glassy carbon electrode (GCE) has been fabricated via an electrochemical oxidation procedure and applied to the electrocatalytic oxidation of reduced form of nicotinamide adenine dinucleotide (NADH). The poly(PyB) film modified electrode surface has been characterized by atomic force microscope (AFM), scanning electron microscope (SEM), electrochemical impedance spectroscopy (EIS), UV-visible absorption spectrophotometry (UV-vis) and cyclic voltammetry (CV). These studies have been used to investigate the poly(PyB) film, which demonstrates the formation of the polymer film and the excellent electroactivity of poly(PyB) in neutral and even in alkaline media. Due to its potent catalytic effects towards the electrooxidation of NADH at lower potential (0.0 V), poly(PyB) film modified electrode can be used for the selective determination of NADH in real samples because of dopamine, ascorbic acid and uric acid oxidation can be avoided at this potential. The catalytic peak currents are linearly dependent on the concentrations of NADH in the range of 1.0 Â 10 À6 to 5.0 Â 10 À4 mol/L with correlation coefficients of 0.999. The detection limits for NADH is 0.5 Â 10 À6 mol/L. Poly(PyB) modified electrode also shows good stability and reproducibility due to the irreversible attachment of polymer film at GCE surface.

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Electroanalysis of NADH Using Conducting and Redox Active Polymer/Carbon Nanotubes Modified Electrodes-A Review

Cited by 135 publications

References 120 publications

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Amperometric Sensor for Detection of the Reduced Form of Nicotinamide Adenine Dinucleotide Using a Poly(pyronin B) Film Modified Electrode

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