Electroanalysis of single-nucleotide polymorphism by hairpin DNA architectures

Abi, Alireza; Ferapontova, Elena E.

doi:10.1007/s00216-012-6633-z

Cited by 32 publications

(20 citation statements)

References 101 publications

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“…1–10 For the label-free detection of oligonucleotides in solution, different fluorescence based detection strategies applied in hybridization probes, 11,12 Taqman probes, 13,14 Scorpions, 15 or molecular beacons (MBs), 16 have been developed. Among the different probes MBs, which have been introduced by Tyagi and Kramer in 1996, are widely used.…”

Section: Introductionmentioning

confidence: 99%

A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

Nöll

2016

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Section: Introductionmentioning

confidence: 99%

A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

Nöll

2016

Analyst

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“…(1) direct detection of hybridisation (label-free), (2) labelling of the target nucleic acid sequences with redox active substances/nanoparticles or (3) signal probes (indirect labels) that intercalate within the stacked base pairs, electrostatically bind to the phosphate backbone or sit within the grooves of the double helix [2,5]. Whilst label-free methods are often desirable for having fewer sample processing steps, they generally do not display the same levels of sensitivity as labelled methods.…”

Section: Introductionmentioning

confidence: 99%

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Regan¹,

Hallett²,

Wong³

et al. 2014

Electrochimica Acta

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In this work we present a novel cobalt complex, [Co(GA) 2 (aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (R ct ) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection.Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

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“…Lot of questions remain to be solved in order that electrochemistry, which proved to be a valuable method for studies of DNA conformational polymorphism , would become useful also for the delicate DNA structures such as the i‐motifs.…”

Section: Resultsmentioning

confidence: 99%

Comparative Electrochemical and Spectroscopic Studies of I‐Motif‐forming DNA Nonamers

et al. 2019

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The paper shows the structural diversity of cytosine (C)‐rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non‐canonical structures involving protonated bases. A distinct secondary structure formed in (C)‐rich sequences, called i‐motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by 1H NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.

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Electroanalysis of single-nucleotide polymorphism by hairpin DNA architectures

Cited by 32 publications

References 101 publications

A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Comparative Electrochemical and Spectroscopic Studies of I‐Motif‐forming DNA Nonamers

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