Electrochemical amplification for Hg(II) quantification by anchoring an enzymatically extended aptamer

Si, Xiaoxi; Tang, Shiyun; Wang, Kunmiao; Zhou, Guofu; Xia, Jianjun; Zhao, Yue; Zhao, Hui; Shen, Qinpeng; Liu, Zhihua

doi:10.1080/00032719.2019.1626415

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…Based on this mechanism for the biosensor, the logarithm of Hg 2+ concentration and DPV peak current had a good linear relationship in the range of 0.01–500 nM, and the detection limit for the prepared sensor was 5 pM. Si and Tang [ 128 ] developed the electrochemical sensor for determination of mercury ions based on specificity of thymine-rich Hg 2+ aptamer and high catalytic activity of template deoxynucleotidyl transferase (TdT). Template-independent TdT improved the sensitivity of sensor by lengthening the aptamer end of 3'-OH by repeating bases, and Hg 2+ aptamer brought selective interaction of thymine mismatched pairs, enhancing the specificity.…”

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

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Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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“…Si et al [ 107 ] reported electrochemical amplification for quantification of Hg 2+ in water samples by anchoring an enzymatically extended aptamer. Similar to the above-mentioned two examples, this strategy is based on the Hg 2+ -induced selective interaction of thymine mismatched pairs in a thymine-rich aptamer DNA to improve the specificity, while template-independent terminal deoxynucleotidyl transferase (TdT) extends the 3′-OH aptamer termini with repeated bases and increased the sensitivity.…”

Section: Food and Water Analysismentioning

confidence: 99%

Recent advances in aptamer applications for analytical biochemistry

Zon

2022

Analytical Biochemistry

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Aptamers are typically defined as relatively short (20–60 nucleotides) single-stranded DNA or RNA molecules that bind with high affinity and specificity to various types of targets. Aptamers are frequently referred to as “synthetic antibodies” but are easier to obtain, less expensive to produce, and in several ways more versatile than antibodies. The beginnings of aptamers date back to 1990, and since then there has been a continual increase in aptamer publications. The intent of the present account was to focus on recent original research publications, i.e., those appearing in 2019 through April 2020, when this account was written. A Google Scholar search of this recent literature was performed for relevance-ranking of articles. New methods for selection of aptamers were not included. Nine categories of applications were organized and representative examples of each are given. Finally, an outlook is offered focusing on “faster, better, cheaper” application performance factors as key drivers for future innovations in aptamer applications.

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“…[15] Si et al proposed a novel electrochemical approach to improve the sensitivity of Hg(II) quantification in a water sample, which was based on the Hg(II) induced selective interaction of thymine mismatched pairs in a thymine-rich aptamer, while template-independent terminal deoxynucleotidyl transferase extended the 3 0 -OH aptamer termini with repeated bases and increased the sensitivity. [16] Photoelectrochemistry (PEC) is an analytical technique developed on the basis of electrochemistry, which is an energy conversion process including the absorption of photons and electron transition by photoelectroactive substances under light irradiation. The PEC method uses light as excitation signal and current or voltage as detection signal, which are different energy forms without disturbance.…”

Section: Introductionmentioning

confidence: 99%

Photoelectrochemical biosensor for lead ion determination based on complementary strand aptamers

Chen

Zhang

Xie

et al. 2021

J Chinese Chemical Soc

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Lead ion causes heavy metal pollution, which can cause serious damage to the ecological environment and human health. In this paper, a simple and sensitive photoelectrochemical method was designed for lead ion detection by using ZnO nanorods as the photoactive substrate and complementary strand aptamers as target recognition element. ZnO nanorods with favorable stability and photoelectric activity can provide a biocompatible microenvironment for aptamer loading. In the presence of lead ion, it would combine with the recog-

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Electrochemical amplification for Hg(II) quantification by anchoring an enzymatically extended aptamer

Cited by 9 publications

References 28 publications

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Recent advances in aptamer applications for analytical biochemistry

Photoelectrochemical biosensor for lead ion determination based on complementary strand aptamers

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