Electrochemical Approach To Detect Apoptosis

Han, Xiaopeng; Liu, Lei; Meng, Fanben; Huang, Junyi; Li, Genxi

doi:10.1021/ac8005268

Cited by 91 publications

(59 citation statements)

References 21 publications

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“…A change of 120 millidegrees represents a surface change of approximately 1 ng mm −2 as described in previous reports [27][28][29]. To obtain well aligned aptamer monolayers [30], 1 mM MCH was injected into two channels for 1 h. As shown in Fig. 4, an increase in the SPR angle was observed, the adsorption of aptamer on SPR gold chip results in a big angle shift of 789 millidegrees.…”

Section: Immobilization Of Apt1 On Gold Chip Surfacementioning

confidence: 63%

Fe3O4@Au nanoparticles as a means of signal enhancement in surface plasmon resonance spectroscopy for thrombin detection

Chen

Zhou

et al. 2015

Sensors and Actuators B: Chemical

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Section: Immobilization Of Apt1 On Gold Chip Surfacementioning

confidence: 63%

Fe3O4@Au nanoparticles as a means of signal enhancement in surface plasmon resonance spectroscopy for thrombin detection

Chen

Zhou

et al. 2015

Sensors and Actuators B: Chemical

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“…Monolayer-modified electrodes have been developed for monitoring the action and activity of wide-ranging and relevant enzymes: matrix metalloproteinases, which are often overexpressed in diseases such as cancer (Fig. 11) [87]; serine proteases, involved in the tightly coordinated coagulation cascade [88,89]; cysteine proteases [90] such as caspase 3, related to cellular apoptosis [91]; HIV-1 protease [92]; integrating metal nanoparticles with carbon nanotubes on a gold electrode [93].…”

Section: Enzyme Substrates and Inhibitorsmentioning

confidence: 99%

Short peptides as biosensor transducers

2011

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This review deals with short peptides (up to 50 amino acids) as biomimetic active recognition elements in sensing systems. Peptide-based sensors have been developed in recent years according to different strategies. Synthetic peptides have been designed on the basis of known interactions between single or a few amino acids and targets, with attention being paid to the presence of peptide motifs known to allow intermolecular self-organization of the sensing peptides over the sensor surface. Sensitive and sophisticated sensors have been obtained in this way, but the use of designed peptides is limited by severe difficulties in their in silico design. Short peptides from random phage display have been selected in a random way from large, unfocussed, and often preexisting and commercially available phage display libraries, with no design elements. Such peptides often perform better than antibodies, but they are difficult to select when the target is a small molecule because of the need to immobilize it with considerable modifications of its structure. Artificial, miniaturized receptors have been obtained from the reduction of the known sequence of a natural receptor down to a synthesizable and yet stable one. Alternatively, binding sites have been created over a designed, stable peptide scaffold. Short peptides have also been used as active elements for the detection of their own natural receptors: pathogenic bacteria have been detected with antimicrobial and cell-penetrating peptides, but key challenges such as detection of bacteria in real samples, improved sensitivity, and improved selectivity have to be faced. Peptide substrates have been conjugated to fluorescent quantum dots to obtain disposable sensors for protease activity with high sensitivity. Ferrocene-peptide conjugates have been used for electrochemical sensing of protease activity.

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“…Several detection methods have taken advantage of this activity and used this tetra-peptide motif to identify caspase-3. Xiao et al [23] have reported the use of a ferrocene-labelled DEVD peptide as an electrochemical reporter for caspase-3 activity. In the absence of caspase-3, a ferrocene oxidation signal was observed.…”

Section: Introductionmentioning

confidence: 99%