Electrochemical impedance spectroscopy for monitoring caspase-3 activity

Hung, Vinci Wing Sze; Veloso, Anthony J.; Chow, Ari M.; Ganesh, Hashwin V. S.; Seo, Kanghoon; Kendüzler, Erdal; Brown, Ian R.; Kerman, Kağan

doi:10.1016/j.electacta.2014.12.115

Cited by 16 publications

(8 citation statements)

References 34 publications

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“…?DE k even (37) where k i denotes the binomial coefficients. Using equation (37) in equation (36), and identifying the terms associated to the fundamental component and to the second order harmonic:…”

Section: Case 2: Nonlinear Behaviour Zonementioning

confidence: 99%

“…This explains why it has been widely used in electrochemical related fields as fuel cells [6][7][8][9][10][11][12], batteries [13][14][15][16][17][18], corrosion [19][20][21][22][23], coatings [24][25][26], electrochemical sensors [27][28] and supercapacitors [29][30][31][32]. This electrochemical technique has also been used in fields that are not traditionally related to electrochemistry as biochemical assays [33][34][35][36], oncology [37][38][39] and immunology [40][41], amongst others.…”

Section: Introductionmentioning

confidence: 99%

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Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems

Giner-Sanz

Ortega

Pérez-Herranz

2016

Electrochimica Acta

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ElsevierGiner-Sanz, JJ.; Ortega Navarro, EM.; Pérez-Herranz, V. (2016). Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems. Abstract:Electrochemical Impedance Spectroscopy (EIS) is an electrochemical measurement technique that has been applied to a broad range of applications. Three conditions must be fulfilled in order to obtain valid EIS measurements: causality, linearity and stationarity. The non fulfilment of any of these conditions may lead to distorted and biased EIS spectra. Consequently, the verification of the four fundamental conditions is mandatory before accepting any results extracted from an EIS spectrum. In this work, a harmonic analysis based method for linearity assessment and noise quantification in EIS measurements is presented, and validated both from an experimental point of view and from a theoretical point of view, for Tafelian systems. It was shown that the presented method was able to quantitatively assess the nonlinearity of the system; and to quantify and characterize the noise. Moreover, the presented method is able to determine the threshold frequency of the system above which the system does not present significant nonlinear effects even for very large perturbation amplitudes.

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Section: Case 2: Nonlinear Behaviour Zonementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems

Giner-Sanz

Ortega

Pérez-Herranz

2016

Electrochimica Acta

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“…The activation of caspase‐3 is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly(adenosine diphosphate ribose) polymerase (PARP), which induces the further destruction of structural and specific proteins, leading to DNA fragmentation and thereby cell apoptosis . Therefore, the sensitive detection of caspase‐3 expression and activity has become an important subject in apoptosis diagnosis to guide therapeutic choices and monitor treatment …”

Section: Caspase‐3 Activationmentioning

confidence: 99%

“…[58] Therefore, the sensitive detection of caspase-3e xpression and activity has become an important subject in apoptosis diagnosis to guide therapeutic choices and monitor treatment. [59,60] Detection methods of caspasee xpression primarily rely on RT-PCR, flow cytometric based analysis, colori-/fluorometric assays,a nd SPR technology. [61][62][63][64] Recently,t ime-resolved FRET technology has been developed to detect caspase-3.…”

Section: Enzyme Activation During Apoptosismentioning

confidence: 99%

Apoptosis Evaluation by Electrochemical Techniques

Yin

2015

Chemistry An Asian Journal

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Apoptosis has close relevance to pathology, pharmacology, and toxicology. Accurate and convenient detection of apoptosis would be beneficial for biological study, clinical diagnosis, and drug development. Based on distinct features of apoptotic cells, a diversity of analytical techniques have been exploited for sensitive analysis of apoptosis, such as surface plasmon resonance, electrochemical methods, flow cytometry, and some imaging assays. Among them, the features of simplicity, easy operation, low cost, and high sensitivity make electrochemical techniques powerful tools to investigate electron-transfer processes of in vitro biological systems. In this contribution, a general overview of current knowledge on various technical approaches for apoptosis evaluation is provided. Furthermore, recently developed electrochemical biosensors for detecting apoptotic cells and their advantages over traditional methods are summarized. One of the main considerations focuses on designing the recognition elements based on various biochemical events during apoptosis.

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“…Electrochemical impedance spectroscopy (EIS) transduction technique has been regarded as a powerful technique for the characterization of electrochemical systems and functionalization of bio-electrodes. [11][12][13] Due to its merits of portability, low-cost, and simplicity, it has also been successfully applied for the analysis of enzymes, 14,15 tumor markers, 16,17 and heavy metals. 18,19 Since the unique DNA hybridization reaction can cause the changes of the charges, conductivity and microenvironment situation of the biointerface, the EIS has also received considerable attention in DNA analysis.…”

Section: Introductionmentioning

confidence: 99%