Electrochemical Aptasensor Based on a Macrocyclic Ligand Bearing Neutral Red

Evtugyn, Gennady; Kostyleva, Veronika; Sitdikov, R. R.; Porfireva, Anna; Savelieva, Maria; Stoikov, Ivan I.; Антипин, И. С.; Hianik, Tibor

doi:10.1002/elan.201100435

Cited by 16 publications

(15 citation statements)

References 40 publications

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“…The kinetics of electron exchange and the influence of modifier were estimated from the dependence of the cathodic potential on the scan rate Equations (2) and (3) [56]:

E_{p} = \frac{2.303 R T}{α n_{a} F} log v + Const

E_{p} = E^{0'} - \frac{R T}{α n_{a} F} ⌊ 0.780 + ln (\frac{D^{1 / 2}}{k^{0}}) + ln {(\frac{α n_{a} F v}{R T})}^{1 / 2} ⌋

…”

Section: Resultsmentioning

confidence: 99%

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Evtugyn

Porfireva

Stepanova

et al. 2013

Sensors

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An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30®. Thiolated aptamer specific to OTA was covalently attached to Au nanoparticles via Au-S bonding. The interaction of the aptamer with OTA induced the conformational switch of the aptamer from linear to guanine quadruplex form followed by consolidation of the surface layer and an increase of the charge transfer resistance. The aptasensor makes it possible to detect from 0.1 to 100 nM of OTA (limit of detection: 0.02 nM) in the presence of at least 50 fold excess of ochratoxin B. The applicability of the aptasensor for real sample assay was confirmed by testing spiked beer samples. The recovery of 2 nM OTA was found to be 70% for light beer and 78% for dark beer.

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“…The kinetics of electron exchange and the influence of modifier were estimated from the dependence of the cathodic potential on the scan rate Equations (2) and (3) [56]:

E_{p} = \frac{2.303 R T}{α n_{a} F} log v + Const

E_{p} = E^{0'} - \frac{R T}{α n_{a} F} ⌊ 0.780 + ln (\frac{D^{1 / 2}}{k^{0}}) + ln {(\frac{α n_{a} F v}{R T})}^{1 / 2} ⌋

…”

Section: Resultsmentioning

confidence: 99%

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Evtugyn

Porfireva

Stepanova

et al. 2013

Sensors

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“…This was shown earlier for electrochemical aptasensor based on the same aptamer and voltammetric detection of the signal [61]. In the ZnO based aptasensor, non-specific adsorption of serum proteins was suppressed by treating the uncovered Au surface with mercaptoethanol.…”

Section: Selectivity Of the Aptasensormentioning

confidence: 92%

Electrochemical Aptasensor Based on ZnO Modified Gold Electrode

et al. 2013

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We developed an electrochemical thrombin aptasensor based on ZnO nanorods functionalized by electrostatically adsorption of 30-mer DNA aptamers. The sensor surface was characterized by AFM and SEM. The surface layer assembling was optimized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with ferricyanide ions as redox markers. The peak current of the ferricyanide and the charge transfer resistance gradually decreased with increasing concentration of thrombin in the range from 3 pM to 100 nM due to formation of aptamer-thrombin complexes and slower diffusion of the marker ions through the surface layer. At optimal conditions, a limit of detection (LOD) of 3 pM for EIS measurements and 10 pM for CV response was calculated from the S/ N = 3.

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“…The thiacalix [4]arenes 1a-c and 2a-c ( Figure 1) were synthesized at the Organic Chemistry Department of Kazan Federal University from appropriate tetraacid derivatives of p-tert-butylthiacalix [4]arene as described in [30]. Briefly, 1 g of the ethyl ether of appropriate acidic derivative of the thiacalix [4]arene was dissolved in 6.6 mL of THF.…”

Section: Reagentsmentioning

confidence: 99%

Cholinesterase Biosensors Based on Screen‐Printed Electrodes Modified with Co‐Phtalocyanine and Polycarboxylated Thiacalixarenes

et al. 2012

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New acetylcholinesterase biosensors have been developed on the base of screen-printed electrodes modified with Co-phtalocyanine and polycarboxylated thiacalixarenes as enzyme support. The biosensors showed improved operational and analytical characteristics and high accuracy of the signal measurement. The biosensors developed made it possible to detect 3-200 ppb of paraoxon and 1-50 ppb of malaoxon with detection limits down to 2 and 0.05 ppb, respectively (incubation time: 15 min). The influence of macrocyclic receptors was related to nonenzymatic accumulation of the analytes within the surface layer and electrostatic interactions with remaining free carboxylic groups of the support. The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. were determined in 15 min incubation step with very low matrix effect in spiked tap and mineral water. -The steric limitations and specific interactions with the substrate and inhibitors specify the positive effect of macrocycles and their structure influence on the biosensor characteristics.

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Electrochemical Aptasensor Based on a Macrocyclic Ligand Bearing Neutral Red

Cited by 16 publications

References 40 publications

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Electrochemical Aptasensor Based on ZnO Modified Gold Electrode

Cholinesterase Biosensors Based on Screen‐Printed Electrodes Modified with Co‐Phtalocyanine and Polycarboxylated Thiacalixarenes

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