Electrochemical Aspects of Electrospray and Laser Desorption/Ionization for Mass Spectrometry

Abonnenc, Mélanie; Qiao, Liang; Liu, Baohong; Girault, Hubert H.

doi:10.1146/annurev.anchem.111808.073740

Cited by 46 publications

(41 citation statements)

References 122 publications

(124 reference statements)

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“…[17] These reactions, besides altering the composition of the solution and affecting the analyte chemical stability,[18] may also generate gas bubbles that could interrupt the spray. [19] To overcome these undesirable side effects and achieve a steady spray, our device is composed predominantly of non-conductive material.…”

Section: Resultsmentioning

confidence: 99%

An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements

Zinck¹,

Stark²,

Wilson³

et al. 2014

ChemistryOpen

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Time series data can provide valuable insight into the complexity of biological reactions. Such information can be obtained by mass-spectrometry-based approaches that measure pre-steady-state kinetics. These methods are based on a mixing device that rapidly mixes the reactants prior to the on-line mass measurement of the transient intermediate steps. Here, we describe an improved continuous-flow mixing apparatus for real-time electrospray mass spectrometry measurements. Our setup was designed to minimize metal–solution interfaces and provide a sheath flow of nitrogen gas for generating stable and continuous spray that consequently enhances the signal-to-noise ratio. Moreover, the device was planned to enable easy mounting onto a mass spectrometer replacing the commercial electrospray ionization source. We demonstrate the performance of our apparatus by monitoring the unfolding reaction of cytochrome C, yielding improved signal-to-noise ratio and reduced experimental repeat errors.

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Section: Resultsmentioning

confidence: 99%

An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements

Zinck¹,

Stark²,

Wilson³

et al. 2014

ChemistryOpen

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“…In LEPA, the electrochemical aspect of ionization methods of MS has been studied. [13] Under this topic, LEPA has used microfluidic chips to combine the electrochemical/chemical redox reactions with MS analysis, [14,15] investigated the photo-electrochemical principle of matrix-assisted laser desorption/ionization (MALDI), [16] and developed a number of ionization devices for MS. [17] Recently, there is a wide interest in the development of ambient ionization methods that produce ions from untreated samples under ambient conditions for high throughput analysis and to maintain the original state of the sample during analysis. [18] Electrostatic spray ionization (ESTASI) is an ambient ionization method, which has been developed by LEPA since 2012 and which is characterized by inducing the spray by electrostatic forces in order to reduce significantly the influence of the applied high voltage (HV) on the sample.…”

Section: Electrostatic Spray Ionization Mass Spectrometrymentioning

confidence: 99%

Analytical Chemistry at the Laboratoire d'Electrochimie Physique et Analytique

Bondarenko

Cortés‐Salazar

Gasilova

et al. 2015

Chimia

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The Laboratoire d'Electrochimie Physique et Analytique (LEPA) has moved to the new Energypolis campus in Sion. This laboratory is involved in energy research in particular by studying charge transfer reactions at soft interfaces and developing interfacial redox electrocatalysis, by pioneering the concept of photo-ionic cells and by integrating redox flow batteries for the production of hydrogen at the pilot scale. Nonetheless, this laboratory has a long tradition in analytical chemistry with the development of microfabrication techniques such as laser photo-ablation, screen-printing and more recently inkjet printing for the design and fabrication of biosensors and immunosensors. As shown in the present review, the laboratory has recently pioneered new technologies for electrochemical and mass spectrometry imaging and for the screening of allergy in patients. The role of the laboratory in the Valais landscape will be to foster the collaboration with the HES to develop teaching and research in analytical chemistry as this field is a major source of employment for chemists.

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“…After drying in ambient conditions, the modified substrate was heated at 400°C for Figure 5. In-source redox reactions during the matrix-assisted laser desorption ionization or nanomaterial-assisted laser desorption ionization process [98,99]. Reproduced with permission from [89].…”

Section: Nanomaterials To Induce In-source Reaction During the Maldi mentioning

confidence: 99%

Nanomaterial-Assisted Laser Desorption Ionization for Mass Spectrometry-Based Biomedical Analysis

Qiao

Liu

2010

Nanomedicine

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Nanomaterials have been widely used to assist laser desorption ionization of biomolecules for mass spectrometry analysis. Compared with classical matrix-assisted laser desorption ionization, strategies based on nanomaterial-assisted ionization generate a clean background, which is of great benefit for the qualitative and quantitative analysis of small biomolecules, such as therapeutic and diagnostic molecules. As label-free platforms, they have successfully been used for high-throughput enzyme activity/inhibition monitoring and also for tissue imaging to map in situ the distribution of peptides, metabolites and drugs. In addition to widely used porous silicon nanomaterials, gold nanoparticles can be easily chemically modified by thiol-containing compounds, opening novel interesting perspectives. Such functionalized nanoparticles have been used both as probes to extract target molecules and as matrices to assist laser desorption ionization for developing new enzyme immunoassays or for studying DNA hybridization. More recently, semiconductor nanomaterials or quantum dots acting as photosensitive centers to induce in-source redox reactions for proteomics and to investigate biomolecule oxidation for metabolomics have been shown to offer new analytical strategies.

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Electrochemical Aspects of Electrospray and Laser Desorption/Ionization for Mass Spectrometry

Cited by 46 publications

References 122 publications

An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements

An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements

Analytical Chemistry at the Laboratoire d'Electrochimie Physique et Analytique

Nanomaterial-Assisted Laser Desorption Ionization for Mass Spectrometry-Based Biomedical Analysis

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