Electrochemical-based ‘‘antibiotsensor’’ for the whole-cell detection of the vancomycin-susceptible bacteria

Dizaji, Araz Norouz; Ali, Zahraa Hussein; Ghorbanpoor, Hamed; Öztürk, Yasin; Akcakoca, Iremnur; Avcı, Hüseyin; Güzel, Fatma Doğan

doi:10.1016/j.talanta.2021.122695

Cited by 23 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Dizaji et al , used the DropSens AT electrodes to detect whole-cell antibiotic-susceptible bacteria. 32 They immobilized thiolated vancomycin molecules on the SPEs and monitored the binding of the vancomycin-susceptible bacteria S. aureus. The LOD of the sensor was 34 CFU mL −1 and the linear range was 10–10 8 CFU mL −1 .…”

Section: Screen Printed Gold Electrodesmentioning

confidence: 99%

Recent advances in gold electrode fabrication for low-resource setting biosensing

2023

View full text Add to dashboard Cite

show abstract

Section: Screen Printed Gold Electrodesmentioning

confidence: 99%

Recent advances in gold electrode fabrication for low-resource setting biosensing

2023

View full text Add to dashboard Cite

show abstract

“…here are also many other potential applications of impedance spectroscopy in microbiology and biomedicine, such as for monitoring cell viability, detecting bacterial infections, and studying the effects of drugs and other compounds on cells and tissues [23][24][25][26] . Also, Impedance spectroscopy enables the detection of significant parameters such as the bacteriostatic or bactericidal characteristics of novel and existing antimicrobial compounds 27 and whole cell detection of vancomycin susceptibility 28 .…”

Section: Electrochemical Phenotypic Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Revolutionizing Phenotypic Antimicrobial Susceptibility Testing: Lightning-Fast Techniques Based on Cutting-Edge Electrochemistry

R¹

2023

Preprint

View full text Add to dashboard Cite

Due to the ongoing concern for the development of rapid and accurate antimicrobial susceptibility testing (AST) methods in the field of clinical microbiology, several novel AST systems have been developed to replace the traditional gold standard AST techniques, which are time-consuming and can lead to delayed susceptibility guided therapy outcomes. In this review, the focus is on the phenotypic assays based on electrochemistry that are used to determine bacterial susceptibility towards antibiotics. Electrochemical techniques have gained significant interest in the field of AST due to their rapidity, sensitivity, and specificity, although it is important to note that some of these methods may have limitations regarding the specific classes of antibiotics and bacteria that they can be applied to, which researchers can address by fine-tuning the techniques or incorporating additional controls. By offering significant advantages over traditional AST methods in terms of rapidity and accuracy, electrochemical phenotypic assays could lead to improved patient outcomes.

show abstract

“…TB does not take up the Gram stain because there is a waxy coat surrounding the cell wall. 7,8 It is a well-known and set up bacterium, which through the years has evolved some of techniques and resistance mechanisms that permit it to propagate between entire human populations. 9 It is expected that TB latently infects less than a quarter of the world's population (around 1.7 billion individuals), 10 and a crucial aspect of its behavior is its slow metabolism, which means it can take a long time to culture and analyze patient samples and draw definitive conclusions about drug resistance and susceptibility profile.…”

Section: Introductionmentioning

confidence: 99%

“…TB is a member of the Mycobacterium genus, which contains pathogens known to cause serious illnesses in mammals, such as leprosy and tuberculosis. TB does not take up the Gram stain because there is a waxy coat surrounding the cell wall 7,8 . It is a well‐known and set up bacterium, which through the years has evolved some of techniques and resistance mechanisms that permit it to propagate between entire human populations 9 .…”

Section: Introductionmentioning

confidence: 99%

Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes

Ghorbanpoor

Akcakoca

Dizaji

et al. 2023

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

One quarter of the global population is thought to be latently infected by Mycobacterium tuberculosis (TB) with it estimated that 1 in 10 of those people will go on to develop active disease. Due to the fact that M. tuberculosis (TB) is a disease most often associated with low‐ and middle‐income countries, it is critical that low‐cost and easy‐to‐use technological solutions are developed, which can have a direct impact on diagnosis and prescribing practice for TB. One area where intervention could be particularly useful is antibiotic susceptibility testing (AST). This work presents a low‐cost, simple‐to‐use AST sensor that can detect drug susceptibility on the basis of changing RNA abundance for the typically slow‐growing M. tuberculosis (TB) pathogen in 96 h using screen‐printed electrodes and standard molecular biology laboratory reactionware. In order to find out the sensitivity of applied sensor platform, a different concentration (108–103 CFU/mL) of M. tuberculosis was performed, and limit of detection and limit of quantitation were calculated as 103.82 and 1011.59 CFU/mL, respectively. The results display that it was possible to detect TB sequences and distinguish antibiotic‐treated cells from untreated cells with a label‐free molecular detection. These findings pave the way for the development of a comprehensive, low‐cost, and simple‐to‐use AST system for prescribing in TB and multidrug‐resistant tuberculosis.

show abstract

Electrochemical-based ‘‘antibiotsensor’’ for the whole-cell detection of the vancomycin-susceptible bacteria

Cited by 23 publications

References 50 publications

Recent advances in gold electrode fabrication for low-resource setting biosensing

Recent advances in gold electrode fabrication for low-resource setting biosensing

Revolutionizing Phenotypic Antimicrobial Susceptibility Testing: Lightning-Fast Techniques Based on Cutting-Edge Electrochemistry

Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes

Contact Info

Product

Resources

About