Electrochemical Behavior of Guanine and Its Determination by Differential Pulse Adsorption Stripping Voltammetry

Sun, Xianxiang; Chi, Yumei; Chen, Hong‐Yuan

doi:10.1006/mchj.1993.1045

Cited by 7 publications

(3 citation statements)

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“…27 Later, complexation of guanine with Hg in differential-pulse adsorption stripping voltammetry to detect guanine at nanomolar levels in the presence of 10-fold excess of guanosine was reported. 28 A similar method using Cu(ii) was also reported with a detection limit of 0.5 nM. [29][30][31] Use of chemically modified electrodes (CME) against conventional mercury electrodes is always beneficial since the CME render certain advantages like lowering of peak potential (E p ) due to catalytic activity.…”

mentioning

confidence: 99%

Simultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides using a chemically modified electrode

Zen¹,

Chang²,

Ilangovan³

1999

Analyst

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Simultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides an a Nafion-ruthenium oxide pyrochlore modified electrode is described. This chemically modified electrode shows very pronounced electrocatalytic effects towards the oxidation of guanine and adenine. The experimental parameters for individual determinations of guanine and adenine on the modified electrode were first optimised. Excellent detection limits (S/N = 3) of 0.86 ng ml(-1) (5.7 nM) and 2.7 ng ml(-1) (20 nM) for guanine and adenine, respectively, were obtained. Simultaneous determination of guanine and adenine in a mixture was also optimised. The denatured calf thymus DNA and yeast RNA showed oxidation peaks corresponding to guanine and adenine with a linear calibration line up to 5 mu g ml(-1) in pH 4.0 phosphate buffer. The detection limit estimated for these nucleic acids were 11.5 ng ml(-1) and 37.7 ng ml(-1) for guanine and adenine, respectively. The new method provides an alternative to estimate the guanine and adenine contents more selectively and sensitively than currently applied techniques

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mentioning

confidence: 99%

Simultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides using a chemically modified electrode

Zen¹,

Chang²,

Ilangovan³

1999

Analyst

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“…Until now, few papers on the mechanism of electrode processes of guanine and acyclovir analogue for adsorption cathodic stripping voltammetry (ACSV) at the mercury electrode have been reported. 26–28 In our paper, using a 2.0 × 10 −3 mol L −1 NaOH solution containing 0.05 ppm of acyclovir after accumulation time (eg, 120 seconds) at −0.40 V, a well-defined cathodic stripping peak occurring at around −0.70 V was observed during scanning in the negative direction. From results obtained by electrochemical methods (such as differential-pulse, linear-scan and linear cyclic), we suggest that the following electrode reaction processes take place during the accumulation and stripping steps:…”

Section: Resultsmentioning

confidence: 73%

Determination of the Antiretroviral Drug Acyclovir in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

2013

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This paper describes a stripping method for the determination of acyclovir at the submicromolar concentration level. This method is based on controlled adsorptive accumulation of acyclovir at thin-film mercury electrode, followed by a linear cyclic scan voltammetry measurement of the surface species. Optimal experimental conditions include a NaOH solution of 2.0 × 10−3 mol L−1 (supporting electrolyte), an accumulation potential of −0.40 V, and a scan rate of 100 mV s−1. The response of acyclovir is linear over the concentration range 0.02 to 0.12 ppm. For an accumulation time of 4 minutes, the detection limit was found to be 0.42 ppb (1.0 × 10−9 mol L−1). More convenient methods to measure the acyclovir in presence of the didanosine, efavirenz, nevirapine, nelfinavir, lamivudine, and zidovudine were also investigated. The utility of this method is demonstrated by the presence of acyclovir together with Adenosine triphosphate (ATP) or DNA.

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“…Until now, few studies on the mechanism of electrode processes of xanthine for adsorption using cathodic stripping voltammetry at thin-film mercury electrode have been reported. Using a mechanism similar to that proposed by Chen and co-workers, we suggest that the following electrode reaction processes take place during the accumulation and stripping steps 17 according to the following equations:

…”

Section: Resultsmentioning

confidence: 93%

Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Farias

Castro

2014

ACI

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A stripping method for the determination of xanthine in the presence of hypoxanthine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10−3 mol L−1 NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and −0.50 V for hypoxanthine–copper, and a linear scan rate of 200 mV second−1. The response of xanthine is linear over the concentration ranges of 20–140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10−10 mol L−1). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of xanthine associated with hypoxanthine, uric acid, nitrogenated bases, ATP, and ssDNA.

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Electrochemical Behavior of Guanine and Its Determination by Differential Pulse Adsorption Stripping Voltammetry

Cited by 7 publications

References 0 publications

Simultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides using a chemically modified electrode

Simultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides using a chemically modified electrode

Determination of the Antiretroviral Drug Acyclovir in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

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