Electrochemical Biosensor Based on surfactant doped polypyrrole (PPy) matrix for lactose determination

Abstract

Lactose biosensor based on surfactant doped polypyrrole (PPy) was developed. Galactose oxidase and β‐galactosidase was coimmobilized in PPy matrix during electropolymerization process with the presence of sodium dodecylbenzene sulphonic acid as surfactant. Bi‐enzyme entrapped PPy was characterized with Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), and scanning electron microscopy (SEM). The response of the enzyme electrode was measured by CV in the range of −0.1 to 1.0 V versus Ag/AgCl which was due to the electrooxidation of enzymatically produced H2O2. The effect of lactose concentration was investigated. Response time of biosensor was found to be 8–10 s (the time required to obtain the maximum peak current) and upper limit of the linear working portions was found to be 1.22 mM lactose concentration with a detection limit of (2.6 × 10−6 M). The apparent Michaelis–Menten constant was found to be 0.117 mM lactose. The effects of interferents (ascorbic acid and uric acid) were determined. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40200.

A novel lactose biosensor based on electrochemically synthesized 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer

“…It was determined that the effect of ascorbic and uric acids on the electrode response was insignificant ( Figure 11). Similar results were reported by Gürsoy et al [51].…”

Development of a new two‐enzyme biosensor based on poly(pyrrole‐co‐3,4‐ethylenedioxythiophene) for lactose determination in milk

“…The sensitivity of enzyme electrode was determined as 1.08 A M −1 cm −2 from the plot of current density (current/area) against concentration. The upper limit of linear working range of developed biosensor is better than that of some studies in literature . Limit of detection is lower than that of .…”

“…To the best of our knowledge, lactose biosensor designed by PEDOT is not available in the literature. Although several articles relating to the designation of biosensors using conductive polymers have been published , articles on the development of lactose biosensors using conductive polymers are rather scarce. In this study, for the first time, we present the preparation of a lactose biosensor using a copolymer of polypyrrole and poly(3,4‐ethylenedioxythiophene), synthesized using an electropolymerization method.…”

Recent advances in analytical techniques for the determination of lactose

“…Gursoy et al [21] developed the lactose biosensor based on surfactant doped polypyrrole. Galactose oxidase and b-galactosidase was coimmobilized in polypyrrole matrix during electropolymerization process with the presence of sodium dodecylbenzene sulphonic acid as surfactant.…”