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Lactose biosensor based on surfactant doped polypyrrole (PPy) was developed. Galactose oxidase and β‐galactosidase was coimmobilized in PPy matrix during electropolymerization process with the presence of sodium dodecylbenzene sulphonic acid as surfactant. Bi‐enzyme entrapped PPy was characterized with Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), and scanning electron microscopy (SEM). The response of the enzyme electrode was measured by CV in the range of −0.1 to 1.0 V versus Ag/AgCl which was due to the electrooxidation of enzymatically produced H2O2. The effect of lactose concentration was investigated. Response time of biosensor was found to be 8–10 s (the time required to obtain the maximum peak current) and upper limit of the linear working portions was found to be 1.22 mM lactose concentration with a detection limit of (2.6 × 10−6 M). The apparent Michaelis–Menten constant was found to be 0.117 mM lactose. The effects of interferents (ascorbic acid and uric acid) were determined. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40200.

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Poly(2-thiophene-3-yl-malonic acid) (P3TMA) was synthesized electrochemically using tetrabutylammonium perchlorate (TBAClO 4 ) as electrolyte into acetonitrile (ACN) for glucose biosensor application. The properties of P3TMA were investigated using FTIR, SEM, and electrochemical measurements. The glucose oxidase enzyme (GOD) was chemically immobilized onto P3TMA modified electrode using glutaraldehyde as crosslinking. It was observed that response current of P3TMA/GOD enzyme electrode increased linearly with loading glucose concentration. Michaelis Menten constant (K m ) of P3TMA/GOD enzyme electrode was calculated as 0.03517 mM. FTIR and SEM analyses were used to confirm immobilization of GOD onto surface of P3TMA-modified electrode.

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