Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification

Zhou, Yunlei; Yang, Zhiqing; Li, Xue; Wang, Yue; Yin, Huanshun; Ai, Shiyun

doi:10.1016/j.electacta.2015.06.043

Cited by 31 publications

(14 citation statements)

References 34 publications

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“…So far, many methods have been developed for the determination of ALP including electrochemical methods [5][6][7] , colorimetry methods [8][9][10] , and fluorescence methods [11][12][13] . Among them, fluorescence methods have attracted great attention due to their high sensitivity.…”

Section: Reasons For Urgent Publicationmentioning

confidence: 99%

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

Xiao

Fang

et al. 2021

ANAL. SCI.

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Section: Reasons For Urgent Publicationmentioning

confidence: 99%

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

Xiao

Fang

et al. 2021

ANAL. SCI.

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“…The production of p-nitrophenol can be detected as it is electrochemically active. 111 Another biosensor for hmC that also utilises alkaline phosphatase has also been developed. In this method DNA strands were first immobilised on a magnetic bead.…”

Section: Detection Of Hmcmentioning

confidence: 99%

“…Biotinylation using an alkyl sulfinate reagent 108 Electrochemical biosensing methods [111][112][113][114][115][116] [Ru(NH3)6] 3+ in electrochemical sensing 117 Labelling with radiolabelled glucose 104 GLIB 25 hMe-Seal 105,106 Nano-hmC-Seal 107 Glucosylation followed by reaction with phenylboronic acid microspheres 109 Glucosylation, reaction with boronic acid, and PCR assay 110 Peroxotungstate 118 Cationic conjugated polymers in FRET based detection 119 Labelling of mC and hmC for FRET assay 120…”

Section: Detection Of Hmcmentioning

confidence: 99%

Methods for detection of cytosine and thymine modifications in DNA

Berney

McGouran

2018

Nat Rev Chem

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Methylation of cytosine at the 5-position is a common epigenetic marker in mammalian DNA, and plays an important role in regulating gene expression. Oxidised derivatives of 5-methylcytosine have recently been discovered. As well as being intermediates in an active demethylation pathway, some of these oxidised derivatives may function as epigenetic markers in their own right. Oxidised derivatives of thymine are also known as products of DNA damage. There is evidence however that one such derivative, 5-hydroxymethyluracil, may play an epigenetic role. There is a pressing need to learn more about these modifications, due to the role epigenetic markers play in development, and diseases such as cancer. This emerging area of research requires methods for detecting cytosine and thymine modifications in DNA with a high degree of accuracy and sequence specificity. This review will introduce the biochemistry of cytosine and thymine modifications, and discuss new and established detection methods which have been developed to overcome the high degree of difficulty associated with studying these modifications in DNA. Introduction: Cytosine and Thymine Modifications DNA codes for all the proteins necessary for life, but the DNA sequence is not the sole determinant of all the phenotypic traits of cells and organisms. Transcription of DNA is tightly regulated by epigenetic modifications, which play an important role in controlling when and where specific genes are expressed. 1,2 Epigenetic modifications regulate important processes such as cellular differentiation, and are also implicated in various diseases including cancer, autism spectrum disorder, and other developmental diseases such as Rett syndrome. 3-5 Epigenetic control of gene expression is often mediated through covalent modification of DNA itself, or of the histone proteins which pack DNA in the nucleus. These modifications do not result in changes to the DNA sequence, but can affect the binding of certain proteins to the DNA, including transcription factors and proteins which regulate chromatin structure. 6-8 In mammals and many other eukaryotes, the most common epigenetic covalent modification of DNA is methylation of cytosine (C) at the 5-position. Such modifications can be inherited, but can also be enzymatically introduced and removed in response to stimuli. 9 5-Methylcytosine (mC) is introduced by the methylation of cytosine residues by DNA-methyltransferases (DNMTs) with an S-adenosylmethionine cofactor. 5-Methylcytosine is most often found in the context of 5'-cytosinephosphate-guanine-3' (CpG) dinucleotides. Regions containing a high density of CpG sequences are found in about 72% of promoters in the human genome. 10 Methylation of these regions causes

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“…This format is similar to ELISA (Enzyme‐linked immunosorbent assay) frequently used in quantifying proteins and small molecules; but instead of forming a colored compound, the product formed can be electroactive. The two most widely used enzymes for this assay are alkaline phosphatase (ALP) and horse‐radish peroxidase (HRP) . Enzyme‐linked electrochemical assays are often used in immobilized and labeled platforms because the enzymes must be spatially near the electrode so that electron transfer can occur between the product of the enzymatic reaction and the electrode itself.…”

Section: Immobilized Electrochemical Dna Biosensorsmentioning

confidence: 99%

Rational Design of Electrochemical DNA Biosensors for Point‐of‐Care Applications

Maslova

Hsing

2017

ChemElectroChem

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Electrochemical DNA biosensors show great potential for the sensitive and sequence‐specific detection of disease pathogens. To date, although there are a large number of published articles showing various sensing strategies of an electrochemical biosensor, only a few of those studies actually reach the commercialization phase, because addressing one technical issue would usually be at the expense of another. Moreover, there are various adoptable formats in electrochemical biosensors. Current approaches may or may not use enzymes, immobilize DNA probes, label the sensing substrates with an electroactive reporter molecule, or any combination of these. Although much has been paid to individual advantages and disadvantages, there are a very limited number Review articles studying and analyzing the synergistic aspects of various detection strategies when combined with each other. It is the aim of this Review to highlight the hotspots for innovation when these strategies are used in tandem, in order to create more streamlined improvements in making a versatile biosensor platform that can be administered at point of care.

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Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification

Cited by 31 publications

References 34 publications

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

Methods for detection of cytosine and thymine modifications in DNA

Rational Design of Electrochemical DNA Biosensors for Point‐of‐Care Applications

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