Electrochemical biosensor for the multiplexed detection of human papillomavirus genes

Civit, Laia; Fragoso, Alex; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2010.06.072

Cited by 48 publications

(32 citation statements)

References 12 publications

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“…Electrochemical experiments were carried out using an Autolab model PGSTAT 12 potentiostat/galvanostat controlled with General Purpose Electrochemical System (GPES) software program (Eco Chemie, now Metrohm Autolab, Utrecht, The Netherlands), equipped with a MUX module that facilitated sequential addressing of the 16 working electrodes. Cleaning of the electrode array, prior to probe immobilisation was performed by a combination of chemical washes and electrochemical treatment, as previously described (Civit et al, 2010;Henry et al, 2009). Once the chip was cleaned it was thoroughly rinsed in Milli-Q water and dried using nitrogen gas.…”

Section: Electrochemical Instrumentationmentioning

confidence: 99%

Modified primers for rapid and direct electrochemical analysis of coeliac disease associated HLA alleles

Joda

Beni

Willems³

et al. 2015

Biosensors and Bioelectronics

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Section: Electrochemical Instrumentationmentioning

confidence: 99%

Modified primers for rapid and direct electrochemical analysis of coeliac disease associated HLA alleles

Joda

Beni

Willems³

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…The actual interest on multiplex assays [31], which refers to the fulfilment of a full range of laboratory tests in the form of a centimeter square microarray, is evident. Also the incorporation of technological advances as microfluidic channels is starting to venture willing to improve the already stablished multiplex assays.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…In addition, there is a notable interest on the development of ultrasensitive methods for the detection of particular papillomavirus strains, especially the high-risk associated HPV types 16, 18, and 45 [31,32,68]. In spite of these electrochemical biosensors being prototypes and requiring additional studies of reproducibility, stability, and validation, they show potential to become valuable alternatives or even the new conventional diagnostic methods [93].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…The main types of signal transduction methods used in biosensors for HPV are optical, electrochemical, and piezoelectric. In addition, the signal conversion can be accomplished through SPR, EIS, and QCM techniques [31,70,103].…”

Section: Biosensors For Hpv As Alternative Diagnostic Toolsmentioning

confidence: 99%

“…The most frequent platform to immobilize the oligonucleotides is a gold electrode, achieving the interaction through thiol-gold covalent bonding. The process can be performed in the presence of a secondary reporter probe (which will serve as a label during the measurement process) as tetramethylbenzidine (TMB), horseradish peroxidase, methylene blue, or hematoxylin providing an amplified electrochemical response combined with greater specificity [29,31,32,36].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

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Trends in Biosensors for HPV: Identification and Diagnosis

et al. 2015

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The conventional methodologies used for the detection of human papillomavirus (HPV) present actually robust and reproducible advantages. However, at the same time, they involve complex protocols that sometimes are difficult to popularize. Over the first half of XX century, the adequate treatment of complex and delicate processes from a simple instrumental base seemed a fundamental and intrinsic contradiction. However, interdisciplinary trends have allowed the manipulation of tissues, proteins, and nucleic acids through innovative increasingly smaller devices. The proper diagnosis of HPV has seen great advances since biosensor researchers are employing its virus strains as models to study the interactions between the biorecognition element and the transducer. Additionally, all recent improvements and trends that material sciences, biotechnology, and data processing scientists excel for biosensors can be applied for the HPV detection platforms. In this review, we highlight the recent trends on materials, nanomaterials, and transducers for the specific detection and differentiation of HPV strains. The most influential methods for the detection and identification of these papillomaviruses include optical, electrochemical, and piezoelectric transducers; we will visit their sensibility and advantages. Additionally, we highlight the factors that contributed to the increasing importance of these biodevices as potential substitutes to conventional diagnostic methods.

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Combination of Mass Signal Amplification and Isotope‐Labeled Alkanethiols for the Multiplexed Detection of miRNAs

Kang

Hong

Sung

et al. 2017

Chemistry An Asian Journal

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We report a fast and sensitive method for the multiplexed detection of miRNAs by combining mass signal amplification and isotope-labeled signal reporter molecules. In our strategy, target miRNAs are captured specifically by immobilized DNAs on gold nanoparticles (AuNPs), which carry a large number of small molecules, called amplification tags (Am-tags), as the reporter for the detection of target miRNAs. For multiplexed detection, we designed and synthesized four Am-tags containing 0, 4, 8, 12 isotopes so that they had same molecular properties but different molecular weights. By observing the mass signals of the Am-tags on AuNPs decorated along with different probe DNAs, four types of miRNAs in a sample could be easily discriminated, and the relative amounts of these miRNAs could be quantified. The practicability of our strategy was further verified by measuring the expression levels of two miRNAs in HUVECs in response to different CuSO concentrations.

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Electrochemical biosensor for the multiplexed detection of human papillomavirus genes

Cited by 48 publications

References 12 publications

Modified primers for rapid and direct electrochemical analysis of coeliac disease associated HLA alleles

Modified primers for rapid and direct electrochemical analysis of coeliac disease associated HLA alleles

Trends in Biosensors for HPV: Identification and Diagnosis

Combination of Mass Signal Amplification and Isotope‐Labeled Alkanethiols for the Multiplexed Detection of miRNAs

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