Electrochemical co-reduction synthesis of Au/ferrocene–graphene nanocomposites and their application in an electrochemical immunosensor of a breast cancer biomarker

Li, Chunxiang; Qiu, Xiyang; Deng, Keqin; Hou, Zhaohui

doi:10.1039/c4ay01838a

Cited by 24 publications

(18 citation statements)

References 40 publications

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“…It can be that there are a large number of gold nanoparticles distributed on the graphene, and these gold nanoparticles can be used for the covalent immobilization of the enzyme. At the same time, the RGOÀ Au/Ni was further analyzed by EDS, and it can be seen that there are C, O, and Au peaks (Figure 2D); all of the above suggest that we had prepared graphene-gold hybrid materials [20].…”

Section: The Characteristics and Structure Of Fabricated Electrodementioning

confidence: 87%

Enzymatic Biosensor Based on One‐step Electrodeposition of Graphene‐gold Nanohybrid Materials and its Sensing Performance for Glucose

Miao¹,

Yan²,

Bi³

et al. 2021

Electroanalysis

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RGO/Au/Ni electrode was manufactured by a convenient, controllable, and environmental process, which was carried out by cyclic voltammetry (CV), and in this process, graphene-gold nanohybrid materials were simultaneously deposited on the nickel foam. Then the GOx was immobilized on the RGO/Au/Ni electrode by covalent bonding, and obtained the enzymatic biosensor. Scanning electron microscope (SEM) and Raman spectroscopy were adopted to confirm the microstructure of the fabricated RGO/Au/Ni electrode. Fourier transform infra-red spectroscopy (FT-IR) was used to characterize the prepared enzymatic electrode. CV, chronoamperometry, and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical performance of the fabricated enzymatic biosensor. It is found that AuNPs were well dispersed on the wrinkled RGO sheets, and the biosensor had a high sensitivity to glucose (32.83 μA • mM À 1 • cm À 2 ) with a wide linear range (0.15�26.15 mM), the strengths of anti-interference ability, good stability, and repeatability, etc.

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Section: The Characteristics and Structure Of Fabricated Electrodementioning

confidence: 87%

Enzymatic Biosensor Based on One‐step Electrodeposition of Graphene‐gold Nanohybrid Materials and its Sensing Performance for Glucose

Miao¹,

Yan²,

Bi³

et al. 2021

Electroanalysis

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“…The amplitude of this signal decreases with increasing antigen concentration because of the electron transfer hindrance caused by the formed immunocomplex. The most commonly employed redox probes in the label-free approach for cancer immunosensors are: [Fe(CN) 6 ] 3À/4À [18,24,25,28,35,46,50,55,57,61,66,87,95,99], Prussian blue (PB) [19,39], Ferrocene (Fc) [34,54], Ferrocenecarboxylic acid (Fc-COOH) [38], Methylene Blue (MB) [62], Cobalt hexacyanoferrate NPs [64], thionine (TH) [79,97] [89,91,92], Azure I [98]. As an example, the [Fe(CN) 6 ] 3À/4À redox probe was used by Deng et al [46] in a label-free immunoassay for trace species analysis, based on the "gate-effect" of a b-cyclodextrin (b-CD) layer.…”

Section: Assay Formats (Immunochemical Interactions)mentioning

confidence: 99%

“…Furthermore, the conjugation between gold‐ and carbon‐based nanomaterials and/or ionic liquids offer multiple options in the sensor's construction. Improved performances were achieved using distinct combinations, such as AuNC/nickel hexacyanoferrate NPs/AuNP‐GS , MWCNTs/AuNPs , AuNPs/Fc‐rGO , AuNPs‐porous GO , MWCNTs/gold colloids , Nafion/MWCNTs/AuNPs , AuNRs/CNTs , gold nanowires (AuNW)/GS , chitosan (CS)‐SWCNTs/AuNPs , rGO/nafion/AuNPs , AuNPs/PDA/TH/GO .…”

Section: Electrode Surface Modificationmentioning

confidence: 99%

“…The amplitude of this signal decreases with increasing antigen concentration because of the electron transfer hindrance caused by the formed immunocomplex. The most commonly employed redox probes in the label‐free approach for cancer immunosensors are: [Fe(CN) 6 ] 3−/4− , , , Prussian blue (PB) , Ferrocene (Fc) , Ferrocenecarboxylic acid (Fc‐COOH) , Methylene Blue (MB) , Cobalt hexacyanoferrate NPs , thionine (TH) , [Ru(NH 3 ) 6 ] 3+ , H 2 O 2 , Azure I . As an example, the [Fe(CN) 6 ] 3−/4− redox probe was used by Deng et al.…”

Section: Assay Formats (Immunochemical Interactions)mentioning

confidence: 99%

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Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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“…The ferrocene complex acted as an electron shuttle allowing for glucose oxidation at low potentials. Li et al prepared a nanocomposite consisting of reduced graphene oxide hybridized with electrochemically co-reduced gold nanoparticles and ferrocene as a sensitive immunosensor of breast cancer biomarkers [ 14 ]. Very recently, Mars et al showed that the aggregation of gold nanoparticles through a ferrocene-based bipodal ligand allowed for the preparation of a new kind of potential-shifting biosensors working differently from classical FET potentiometric sensors [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

Argoubi¹,

Saadaoui²,

Aoun³

et al. 2015

Beilstein J. Nanotechnol.

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SummaryThe demand for on-site nanodevices is constantly increasing. The technology development for the design of such devices is highly regarded. In this work, we report the design of a disposable platform that is structured with cauliflower-shaped gold nanoparticles (cfAuNPs) and we show its applications in immunosensing and enzyme-based detection. The electrochemical reduction of Au(III) allows for the electrodeposition of highly dispersed cauliflower-shaped gold nanoparticles on the surface of screen-printed carbon electrodes (SPCEs). The nanostructures were functionalized using ferrocenylmethyl lipoic acid ester which allowed for the tethering of the ferrocene group to gold, which serves as an electrochemical transducer/mediator. The bioconjugation of the surface with anti-human IgG antibody (α-hIgG) or horseradish peroxidase (HRP) enzyme yields biosensors, which have been applied for the selective electrochemical detection of human IgG (hIgG) or H2O2 as model analytes, respectively. Parameters such as the number of sweeps, amount of charge generated from the oxidation of the electrodeposited gold, time of incubation and concentration of the ferrocene derivatives have been studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Selectivity and specificity tests have been also performed in the presence of potentially interfering substances to either hIgG or H2O2. Results showed that the devised immunosensor is endowed with good selectivity and specificity in the presence of several folds of competitive analytes. The enzyme-based platform showed a good catalytic activity towards H2O2 oxidation which predestined it to potential applications pertaining to enzymatic kinetics studies. The levels of hIgG in human serum and H2O2 in honey were successfully determined and served as assessment tools of the applicability of the platforms for real samples analysis.

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Electrochemical co-reduction synthesis of Au/ferrocene–graphene nanocomposites and their application in an electrochemical immunosensor of a breast cancer biomarker

Cited by 24 publications

References 40 publications

Enzymatic Biosensor Based on One‐step Electrodeposition of Graphene‐gold Nanohybrid Materials and its Sensing Performance for Glucose

Enzymatic Biosensor Based on One‐step Electrodeposition of Graphene‐gold Nanohybrid Materials and its Sensing Performance for Glucose

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

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