Electrochemical Detection in a Paper-Based Separation Device

Carvalhal, Rafaela Fernanda; Kfouri, Marta Simão; Piazetta, Maria Helena de Oliveira; Gobbi, Ângelo L.; Kubota, Lauro T.

doi:10.1021/ac902647r

Cited by 205 publications

(131 citation statements)

References 18 publications

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“…19 Our group exploited the advantage of filter paper to develop a paper disk impregnated with reagents on SPCE for a trace metal assay, Pb(II) could be measured with one-step method based on internal standard.…”

Section: -15mentioning

confidence: 99%

“…18 Paper-based separation device was applied for the separation and quantification of uric and ascorbic acid. 19 Our group exploited the advantage of filter paper to develop a paper disk impregnated with reagents on SPCE for a trace metal assay, Pb(II) could be measured with one-step method based on internal standard. 11,12 Paper-based integration created a multifunctional device that can be applied in point-of-care testing in environmental or industrial monitoring of different analytes.…”

Section: 10mentioning

confidence: 99%

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Electrochemical detection of Pb and Cd in paper-based microfluidic devices

Shi¹,

Tang²,

Xing³

et al. 2012

J. Braz. Chem. Soc.

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Tiras de papel de filtro combinadas com eletrodos de carbono impresso (SPCE) foram usadas na montagem de um dispositivo microfluídico para detecção de Pb(II) and Cd(II) em soluções aquosas. O dispositivo microfluídico portátil (com detecção eletroquímica direta) foi testado na quantificação direta de analitos (Pb(II) e Cd(II)) em amostras aquosas contaminadas sem pré-tratamentos. O sinal voltamétrico de redissolução anódica de onda quadrada (SWASV) foi bastante intensificado e apresentou excelente desempenho analítico na detecção de Pb(II) e Cd(II) (de 0 a 100 ppb) com baixo limite de detecção (2,0 e 2,3 ppb, respectivamente). Os dispositivos eletroquímicos propostos também apresentaram boa seletividade e estabilidade para análises de amostras reais de refrigerante e água subterrânea com contaminação física. O dispositivo é simples, de baixo custo, fácil fabricação e portátel. Este dispositivo analítico tem a possibilidade de ser útil em aplicações do tipo point-of-care no monitoramento ambiental, na saúde pública e na segurança alimentar.Filter paper strips combined with screen-printed carbon electrodes (SPCE) were used in the assembly of a microfluidic device for detecting Pb(II) and Cd(II) in aqueous samples. The portable microfluidic device (with direct electrochemical detection) was tested for direct quantification of multiple analytes (Pb (II) and Cd(II)) in contaminated aqueous samples without pretreatment. Square wave anodic stripping voltammetric (SWASV) signal was greatly enhanced and displayed excellent analytical performance for Pb(II) and Cd(II) detection (from 0 to 100 ppb) with low limit of detection of 2.0 and 2.3 ppb, respectively. The proposed electrochemical devices also exhibited good selectivity and stability for analyses of real samples of gas dissolved salty soda water and ground water with physical contamination. The device is simple, low-cost, easy-to-fabricate and portable. This analytical device has the possibility of being useful for point-of-care applications in environmental monitoring, public health and food safety.Keywords: microfluidic devices, screen-printed carbon electrodes, filter paper strip, portable device IntroductionAnalytical instrumentations tend to be miniaturization and intellectualization for in situ applications.1-4 Therefore, it can be convenient for real-time and on-line monitoring in the fields of environmental monitoring, biomedical science, agriculture, military analysis and industrial process monitoring, etc. Paper represents a great and useful supporting material for developing sensing devices owing to its various unique advantages, such as inexpensive, easy-printed, biodegradable and the porous structure facilitates lateral-flow assays suitable for chromatographic separations and as good filter.5-8 Screen-printing technology can be easily applied to the mass production of inexpensive, reproducible and sensitive disposable electrodes. 9,10Paper-based micro-electrochemical device integrated screen-printed carbon electrodes (SPCE) can meet the requirements fo...

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Section: -15mentioning

confidence: 99%

Section: 10mentioning

confidence: 99%

Electrochemical detection of Pb and Cd in paper-based microfluidic devices

Shi¹,

Tang²,

Xing³

et al. 2012

J. Braz. Chem. Soc.

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“…Biosensors with ChrPr provide advantages in low cost and disposability. [1][2][3][4] Biosensors with AuNPs also provide some advantages in helping electron transport or mediation on the sensor electrodes, 5 and a surface plasmon resonance effect used for cancer detection. 6,7 One of the examples of the bioassay exploiting ChrPr and AuNPs is the immunochromatographic strip test (IST), which is a simple and rapid flowing assay 8 used for tests of pregnancy, 9 influenza 10 and cancer tumor.…”

Section: Introductionmentioning

confidence: 99%

“…5,[22][23][24][25][26] CA and EIS are especially adequate for the latest research related to the electrical characteristics of unique paper. 3,[27][28][29] The latest IST research is no exception to the use of CA and EIS. The measurement principle of the quantitative IST using CA is an oxidation-reduction reaction occurring on the modified electrodes underneath the detection area.…”

Section: Introductionmentioning

confidence: 99%

Impedance Analysis of Colloidal Gold Nanoparticles in Chromatography Paper for Quantitation of an Immunochromatographic Assay

et al. 2016

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A detection method of gold nanoparticles in chromatography paper has been developed for a simple, cost-effective and reliable quantitation of immunochromatographic strip test. The time courses of the solution resistance in chromatography paper with the gold nanoparticles solution are electrochemically measured by chrono-impedimetry. The dependence of the solution resistance on the concentration of gold nanoparticles has been successfully observed. The main factor to increase the solution resistance may be obstruction of the ion transport due to the presence of gold nanoparticles. The existence of gold nanoparticles with 1.92 × 10 9 particles/mL in an indistinctly-colored chromatography paper is also identified by a solution resistance measurement. This indicates that the solution resistance assay has the potential to lower the detection limit of the conventional qualitative assay.Keywords Immunochromatography, chromatography paper, gold nanoparticles, solution resistance

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“…Microfluidic paper-based electrochemical devices (μPEDs), which are categorized in μPADs with electrochemical systems, were applied to detect glucose, [7][8][9][10] lactate, 8,10 cholesterol, 8 uric acid, 10,11 and ascorbic acid 11 based on enzyme reactions, and may be used for immunosensing shortly.…”

Section: Introductionmentioning

confidence: 99%

A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine

2015

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A dual-electrochemical sensor based on a test-strip assay with immunochemistry and enzyme reactions has been developed for the determination of albumin and creatinine. Each nitrocellulose membrane with an immobilization area of an anti-albumin antibody or three enzymes was prepared in the device with three working electrodes for measuring albumin, creatinine, and ascorbic acid, as well as an Ag/AgCl electrode used as a counter/pseudo-reference electrode. The reactions of three enzymes were initiated by flowing a solution containing creatinine to detect an oxidation current of hydrogen peroxide. A sandwich-type immunocomplex was formed by albumin and antibody labeled with glucose oxidase (GOx). Captured GOx catalyzed the reduction of Fe(CN)6 3-to Fe(CN)6 4-, which was oxidized electrochemically to determine the captured albumin. The responses for creatinine and albumin increased with the concentrations in millimolar order and over the range 18.75 -150 μg mL -1 , respectively. The present sensor would be a distinct demonstration for producing quantitative dual-assays for various biomolecules used for clinical diagnoses.

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Electrochemical Detection in a Paper-Based Separation Device

Cited by 205 publications

References 18 publications

Electrochemical detection of Pb and Cd in paper-based microfluidic devices

Electrochemical detection of Pb and Cd in paper-based microfluidic devices

Impedance Analysis of Colloidal Gold Nanoparticles in Chromatography Paper for Quantitation of an Immunochromatographic Assay

A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine

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