Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Kim, Yeon Seok; Jung, Ho‐Sup; Matsuura, Toshihiko; Lee, Hea Yeon; Kawai, Tomoji; Gu, Man Bock

doi:10.1016/j.bios.2006.10.004

Cited by 249 publications

(142 citation statements)

References 39 publications

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“…Therefore, these changes may have affected the diffusion of redox-mediator and thus change in the current, which was measured by CV and SWV [46,47]. A linear relationship between the logarithmic TET concentration and current change (DI) was plotted (R 2 = 0.955), and dynamic range was determined to be in the range 10 nM-10 lM of TET (Fig.…”

Section: Quantitative Detection Of Tetracyclinementioning

confidence: 99%

Electrochemical aptasensor for tetracycline detection

Kim

Niazi

et al. 2009

Bioprocess Biosyst Eng

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164

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An electrochemical aptasensor was developed for the detection of tetracycline using ssDNA aptamer that selectively binds to tetracycline as recognition element. The aptamer was highly selective for tetracycline which distinguishes minor structural changes on other tetracycline derivatives. The biotinylated ssDNA aptamer was immobilized on a streptavidin-modified screen-printed gold electrode, and the binding of tetracycline to aptamer was analyzed by cyclic voltammetry and square wave voltammetry. Our results showed that the minimum detection limit of this sensor was 10 nM to micromolar range. The aptasensor showed high selectivity for tetracycline over the other structurally related tetracycline derivatives (oxytetracycline and doxycycline) in a mixture. The aptasensor developed in this study can potentially be used for detection of tetracycline in pharmaceutical preparations, contaminated food products, and drinking water.

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Section: Quantitative Detection Of Tetracyclinementioning

confidence: 99%

Electrochemical aptasensor for tetracycline detection

Kim

Niazi

et al. 2009

Bioprocess Biosyst Eng

Self Cite

164

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“…The electrochemical approach is cost-effective because it does not require expensive instrumentation [24,25] and would allow portability [26]. FIS transduction is especially well suited to detect changes that occur in the solution-electrode interface without the need of labels.…”

Section: Introductionmentioning

confidence: 99%

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Queirós

de‐los‐Santos‐Álvarez

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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a b s t r a c tA label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs) was developed. Two single stranded DNA sequences were tested as recognition elements and compared. The aptamer capture probes were immobilized, with and without 6-mercapto-1-hexanol (MCH) on a gold electrode. Each step of the modification process was characterized by Faradaic impedance spectroscopy (FIS). A linear relationship between the electron-transfer resistance (R et ) and E. coli OMPs concentration was demonstrated in a dynamic detection range of 1 × 10 −7 -2 × 10 −6 M. Moreover, the aptasensor showed selectivity despite the presence of other possible water contaminates and could be regenerated under low pH condition. The developed biosensor shows great potential to be incorporated in a biochip and used for in situ detection of E. coli OMPs in water samples.

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“…Jenison group and Kato group had obtained ten stands of aptamer for E2 using the equilibrium-filtration method [20,21]. Kim group selected one stand with a dissociation constant of 0.13 lM to detect E2 using electrochemical method [22].…”

Section: Introductionmentioning

confidence: 99%

A novel separation and enrichment method of 17β-estradiol using aptamer-anchored microbeads

Huy

Jin

Yin

et al. 2010

Bioprocess Biosyst Eng

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The estrogenic compound 17b-estradiol (E2) is widely studied for its potential endocrine disruption effects. Due to the low level of E2 present in the environment, it is highly desirable to develop a sensitive and efficient separation and enrichment method for E2 analysis. In this paper, we proposed a novel E2 preconcentration method using anti-E2 aptamer-anchored isothiocyanate-modified beads (NCS beads). The glass beads are chemically modified with primary amino group, and then treated with phenylene diisothiocyanate (PDITC) to generate an isothiocyanate group, which is reactive towards the amine group. The amino-modified anti-E2 aptamer can be easily covalently immobilized onto the as-prepared NCS beads. The experimental results demonstrated that the aptamer affinity microbeads could selectively retain and separate E2 compound. The effects of the operation parameters on retention of E2, including washing condition, eluting condition, the number of beads, and the incubation time were investigated. Moreover, high-performance liquid chromatography with preconcentration of E2 on the aptamer affinity microbeads was applied to detect the E2 in the spiked water samples and obtained a good recovery.

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Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Cited by 249 publications

References 39 publications

Electrochemical aptasensor for tetracycline detection

Electrochemical aptasensor for tetracycline detection

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

A novel separation and enrichment method of 17β-estradiol using aptamer-anchored microbeads

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