Electrochemical detection of a pathogenic Escherichia coli specific DNA sequence based on a graphene oxide–chitosan composite decorated with nickel ferrite nanoparticles

Abstract

In this report, an electrochemical genosensor has been fabricated for Escherichia coli O157:H7 (E. coli) detection using a graphene oxide-nickel ferrite-chitosan (GO/NiF/ch) nanocomposite film as the sensing platform. The prepared GO/NiF/ch nanocomposite was characterized by scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and thermo-gravimetric analysis. Nucleic acid hybridization technique was employed for the detection of a specific sequence of E. coli. The hybridization between the complementary DNA and probe DNA was investigated by differential pulse voltammetry (DPV) using methylene blue as redox indicator. The fabricated biosensor exhibits a linear response to complementary DNA in the concentration range of 10 À6 to 10 À16 M with a detection limit of 1 Â 10 À16 M.

A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

“…Oxidation labels function as hybridization markers that intercalate to double-stranded DNA and gives electrochemical or optical signals. Tiwari et al [4] explored an electrochemical genosensor for E. coli detection based on graphene oxide, nickel ferrite and chitosan, with methylene blue as the redox indicator. Ariffin et al [5] developed an electrochemical DNA biosensor for E. coli detection based on hollow silica spheres (HSMs), using an anthraquinone redox intercalator as the electroactive DNA hybridization label.…”

Electrochemical Genosensors: Definition and Fields of Application

“…Based on oligonucleotides probe that was identified from the 16s rRNA coding region of the Escherichia coli O157:H7 genome, Pandey et al 19 have coupled it with cystine flower-gold particles matrice to improve its immobilization, their genosensor exhibited a linear response with LOD of 1x10 -15 M provide a selective tool for Escherichia coli O157:H7. For the same strain of E. coli, the uses of nanocomposites can improve the detection and decrease the LOD form 10-6 to 10 -16 M. 5 The efficiency of use of electrochemical genosensors was shown in several virus detections such as HIV, 20 papllivirus, 21 dengue virus 4 and hepatitis virus. [22][23] Dengue fever has become a global health concern, as it is the most prevailing vector-borne disease causing predominant mortality and morbidity.…”

“…The electrochemical DNA-based genosensors can be coupled with nanoparticles or nanocomposites to improve both oligonucleotides sequence immobilization on the transducer surface and sensitivity to hybridization. [3][4][5][6][7] The electrochemical techniques applied in genosensor can be used in different goals, e.g Differential Pulse Voltammetry (DPV) as analystical technique is usually used to measure the concentration of some specific electroactive species with high sensitivity. 8 This paper is devoted to giving a general idea about the fields of application of electrochemistry, such as medicine, plant breeding, food safety and quality control, and bacterial and viral analysis.…”

A Voltammetric Sensor Based on MgFe2O4 Decorated on Reduced Graphene Oxide‐modified Electrode for Sensitive and Simultaneous Determination of Catechol and Hydroquinone

“…reported application of manganese ferrite for non‐enzymatic peroxide sensing , Tiwari et al. proposed electrochemical detection of pathogenic Escherichia coli specific DNA sequence based on composite including nickel ferrite nanoparticles , Wang et al. reported application of copper ferrites decorated on reduced graphene oxide as anode materials for advanced lithium storage applications , while Zhu et al.…”