Electrochemical detection of interaction between capsaicin and nucleic acids in comparison to agarose gel electrophoresis

Yilmaz, Nilay; Ekşin, Ece; Karacicek, Bilge; Erac, Yasemin; Erdem, Arzum

doi:10.1016/j.ab.2017.07.023

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…The gel retardation method has been extensively used to evaluate structural variations in the dsDNA molecule, from interactions of numerous chemical species‐DNA [44–46]. Thus, conventional agarose gel electrophoresis was performed, as described in Section 2.4 , to investigate structural DNA variations after exposure to the 3‐NO 2 ‐Tyr molecule.…”

Section: Resultsmentioning

confidence: 99%

Voltammetric Studies of 3‐Nitro‐tyrosine Electro‐oxidation at Solid Electrodes and its Interaction with DNA

Nascimento

Mendes

et al. 2020

Electroanalysis

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The electrochemical oxidation of 3‐nitro‐tyrosine (3‐NO2‐Tyr) was studied in aqueous media at metallic electrodes (platinum and gold), using voltammetric techniques. The interaction between 3‐NO2‐Tyr and double helix DNA (dsDNA) in a physiological medium was also investigated. Electro‐oxidation of 3‐NO2‐Tyr occurs in one single irreversible pH‐dependent step with the transfer of one electron and one proton from the phenolic group to the formation of radicals, which preferably dimerize, fouling the electrode surfaces. The differential pulse voltammetry and gel electrophoresis results clearly demonstrated a strong interaction of 3‐NO2‐Tyr with the dsDNA for the formation of a stable 3‐NO2‐Tyr‐dsDNA complex.

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Section: Resultsmentioning

confidence: 99%

Voltammetric Studies of 3‐Nitro‐tyrosine Electro‐oxidation at Solid Electrodes and its Interaction with DNA

Nascimento

Mendes

et al. 2020

Electroanalysis

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“…It is an irritant and neurotoxin chemical for mammals, including humans, and produces a burning sensation in any tissue it comes into contact with. The interaction between DNA and CPS was investigated using PGE and DPV as shown in Figure 7 [ 120 ]. In addition, voltammetric determination of the interaction between CPS and dsDNA, cDNA from Huh7 human hepatocellular carcinoma cell line, and total RNA isolated from PCR samples was performed and electrophoretic studies were also conducted in order to confirm their results.…”

Section: Electrochemical Detection Of Drug–dna Interactionsmentioning

confidence: 99%

Electrochemical DNA biosensors developed for the monitoring of biointeractions with drugs: a review

GÜRSAN,

ŞENTÜRK,

GÖZ

et al. 2023

Turkish Journal of Chemistry

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The interaction of drugs with DNA is important for the discovery of novel drug molecules and for understanding the therapeutic effects of drugs as well as the monitoring of side effects. For this reason, many studies have been carried out to investigate the interactions of drugs with nucleic acids. In recent years, a large number of studies have been performed to electrochemically detect drug–DNA interactions. The fast, sensitive, and accurate results of electrochemical techniques have resulted in a leading role for their implementation in this field. By means of electrochemical techniques, it is possible not only to demonstrate drug–DNA interactions but also to quantitatively analyze drugs. In this context, electrochemical biosensors for drug–DNA interactions have been examined under different headings including anticancer, antiviral, antibiotic, and central nervous system drugs as well as DNA-targeted drugs. An overview of the studies related to electrochemical DNA biosensors developed for the detection of drug–DNA interactions that were reported in the last two decades in the literature is presented herein along with their applications and they are discussed together with their future perspectives.

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“…Regarding an improvement of the detection efficiency, high resolution of the DNA oxidation products achieved using a boron‐doped diamond electrode as the transducer , nanostructured interfaces using carbon nanotubes e. g., , graphene oxide e. g., , nanoparticles e. g., , polymers, e. g., and a comprehensive electrochemical biosensing approach using the combination of several electrochemical methods, namely cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with a redox‐active indicator, and square‐wave voltammetry of intrinsic signals of the nucleobases can be mentioned. For a comparison, electrophoresis in agarose gel is often used as the standard and widely accepted technique of DNA cleavage monitoring . Strand breaks and degradation of the plasmid‐like structure through its individual forms are visible in agarose gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

et al. 2017

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Different sensitivity of various types of DNA to damage and mutation requires a study of effects of chemical and physical factors on integrity of individual DNA structures. Oxidation stress induced by photoactivated nanoparticles including quantum dots is considered to be the main mechanism of their potential risks. In this work, spectrophotometric indicators of reactive oxygen species (ROS) 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD−Cl) and α‐diphenyl‐β‐picrylhydrazyl (DPPH), electrochemical DNA‐based biosensors with the thiol‐capped CdS quantum dots (CdS QDs) immobilized over the chromosomal (ct DNA) and plasmid (pUC19) DNA biorecognition layers and agarose gel electrophoresis were used (i) to detect ROS generated at the UV‐C irradiation (λ=254 nm) of CdS QDs and (ii) to assess and evaluate their effect towards the chromosomal and plasmid dsDNA structure. Voltammetric and impedimetric measurements revealed a deep degradation of DNA attached to the glassy carbon electrode surface with a stepwise decrease of the portion of survived pUC19 and ct DNA after 60 s to 2400 s irradiation time up to 47 % and to 32 %, resp., comparing to the portion in the absence of CdS QDs reaching 58 % and 40 %, resp. Agarose gel electrophoresis supported the damage to plasmid and chromosomal DNA demonstrating double strand breaks. A decrease in the stability of DNA types towards degradation by ROS in order pUC19>calf thymus>salmon sperm dsDNA has been found.

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Electrochemical detection of interaction between capsaicin and nucleic acids in comparison to agarose gel electrophoresis

Cited by 9 publications

References 42 publications

Voltammetric Studies of 3‐Nitro‐tyrosine Electro‐oxidation at Solid Electrodes and its Interaction with DNA

Voltammetric Studies of 3‐Nitro‐tyrosine Electro‐oxidation at Solid Electrodes and its Interaction with DNA

Electrochemical DNA biosensors developed for the monitoring of biointeractions with drugs: a review

Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

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