Electrochemical Detection of Melatonin at Tungsten Oxide Nanospheres Decorated Chitosan Electrode

Abstract

Melatonin is an important hormone that is clinically significant due to its physiological role, making its detection in real samples crucial for monitoring body function. Compared to other traditional approaches, electrochemical sensors have advantages such as high sensitivity, point-of-care analysis, rapid response time, ease of use, and cost-effectiveness. Recently, natural polymer-based biocomposites such as chitosan, gum acacia, xanthan gum, and chitinhave been employed due to their low cost, biocompatibility, and high surface area for biosensing applications. We have investigated Tungsten oxide (WO3) nanospheres decorated with functionalized chitosan (FCH) for the detection of melatonin. The functionalization of chitosan introduced plentiful amine groups and inter-hydrogen bonding provided necessary stability for the formation of WO3/FCH biocomposite. Further, the electroanalysis confirmed the excellent electrocatalytic performance of the biocomposite towards melatonin in with a limit of detection of 4.9 nM. The proposed nanocomposite exhibited excellent selectivity, reproducibility, stability, and its practical reliability was evaluated real samples. Herein, functionalization of introduced large density of amine groups that offered efficient binding affinity with WO3.and improved the conductivity of the nanocomposite for a highly sensitive melatonin detection. The proposed modified electrode will be a suitable platform for futuristic clinical biosensing applications.

Electrochemical Detection of Hormones Using Nanostructured Electrodes

“…The proposed nanocomposite was selective, reproducible, and stable. Its actual applicability was tested using real samples, which proved its effectiveness in detecting therapeutically significant biomolecules [128].…”