Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA and E. coli DNA ligase

He, Xiaoxiao; Ni, Xiaoqi; Wang, Yonghong; Wang, Kemin; Jian, Lixin

doi:10.1016/j.talanta.2010.10.051

Cited by 17 publications

(8 citation statements)

References 40 publications

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“…Escherichia coli O157:H7 is one of the most dangerous bioorganisms associated with food-borne diseases [173]. Bright fluorescent NPs as labeling could effectively improve the detection sensitivity.…”

Section: Virus and Bacteriamentioning

confidence: 99%

Fluorescent nanoparticles for chemical and biological sensing

Liu

Yang

et al. 2011

Sci. China Chem.

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Fluorescent nanoparticles (NPs), including quantum dots (QDs), dye-doped NPs, and rare earth-based NPs, etc., have been a major focus of research and development during the past decade. The impetus behind such endeavors can be attributed to their unique chemical and optical properties, such as bright fluorescence, high photostability, large Stocks shift and flexible processability. The introduction of fluorescent NPs into analytical chemistry has opened up new venues for fluorescent analysis. In this review, we focus on the developments and analytical applications of fluorescent NPs in the chemical and biological sensing of pH, ions, organic compounds, small biological molecules, nucleic acids, proteins, virus and bacteria. The review also points out the in vitro and in vivo imaging application of fluorescent NPs at the cell and body levels. Meanwhile, the advantages of NPs brought field of sensing and signal transductions are also discussed. fluorescence, sensing, quantum dots, dye-doped nanoparticles, rare earth-based nanoparticles

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Section: Virus and Bacteriamentioning

confidence: 99%

Fluorescent nanoparticles for chemical and biological sensing

Liu

Yang

et al. 2011

Sci. China Chem.

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“…A wide range of biophysical chemistry approaches have been used to detect the joining of DNA strand breaks. Those applied to NDLs or ADLs from bacteria or archaea include use of fluorescently-labelled probes [ 66 , 70 , 72 , 73 ], FRET [ 69 , 74 – 77 ], fluorescence quenching and molecular beacon-based approaches [ 78 – 81 ], electrochemical methods [ 67 , 79 , 81 – 84 ], a nanoparticle-based sensor [ 85 ] and surface plasmon resonance [ 86 ]. Biochemical analyses of the ligation reaction are facilitated by assays that do not require ‘labelling’ of the nucleic acid, such as label-free electrochemical approaches using mercury-based electrodes and nicked plasmid DNA substrates [ 67 ].…”

Section: Assays Of Dna Joining By Dna Ligasesmentioning

confidence: 99%

“…If the other end of the stem-loop contains a label, then the absence or presence of the label can be used to detect alteration to the backbone structure of DNA. By inclusion of a nick in DNA stem-loop structures, then retention of the signal indicates DNA ligation and such approaches have been monitored using fluorescence [ 66 , 78 , 79 ] or electrochemical [ 81 , 82 ] techniques. Interestingly, use of appropriate substrates in these assays allows study of the combined action of nucleases and ligases [ 66 ], which allows extension of these assays to assess links between DNA ligases and other proteins involved in nucleic acid metabolism, as observed in DNA repair processes.…”

Section: Assays Of Dna Joining By Dna Ligasesmentioning

confidence: 99%

Biochemical and structural characterization of DNA ligases from bacteria and archaea

2016

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“…Many schemes for enzyme determination involving MBs utilise the DNA cleavage processes induced by nucleases (e.g. S1 nuclease, DNase I and mung bean nuclease) (Li et al, 2000b;Ma et al, 2008), DNA methylation by methylating enzymes and their inhibitors (Li et al, 2007;Ye & Stivers, 2010;Wood et al, 2010), DNA ligation catalysed by ligases Ma et al, 2008;He et al, 2011), DNA glycosylase removal of uracil bases inducing strand release , DNA phosphorylation , etc. A label-free fluorescence turn-on assay for the detection of endonuclease (Zhou et al, 2012a) has been designed based on G-quadruplex DNAzyme signal amplification.…”

Section: Enzyme Detection Using Molecular Beaconsmentioning

confidence: 99%

Biosensors based on molecular beacons

Stobiecka

Chałupa²

2015

Chemical Papers

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A fundamental molecular beacon (MB) consists of a special short nucleic acid strand with a fluorophore-quencher pair attached to its ends. It provides a unique framework that is susceptible to conformational transitions between a hairpin (closed) conformation and an extended (open) conformation. These two conformations are readily discernible because of their differing fluorescence emission characteristics. The broad applicability of the robust MB sensing platform has attracted widespread interest, resulting in extensive research studies ranging from theoretical and bioanalytical chemistry to molecular biology and biomedical applications. In this paper, the principles of MB design and the modes and mechanisms of MB operation are reviewed, including MB modifications based on the utilisation of a thymidine-thymidine mismatch in hybridised MB stem, aptamers, peptides and locked nucleic acid strands in an MB loop, as well as plasmonic quenchers, quantum dots and interactions with graphene and graphene oxide. Specific applications of MBs in the analysis of enzymes, DNA mutation, phosphorylation, methylation and ligation, followed by the detection of pathogens and applications in cancer and other disease diagnostics and therapeutics are also reviewed. Molecular beacon-based sensing platforms are expanding rapidly and offer a promising bioanalytical tool for inexpensive and reliable analysis for research and field diagnostics.

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Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA and E. coli DNA ligase

Cited by 17 publications

References 40 publications

Fluorescent nanoparticles for chemical and biological sensing

Fluorescent nanoparticles for chemical and biological sensing

Biochemical and structural characterization of DNA ligases from bacteria and archaea

Biosensors based on molecular beacons

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