Electrochemical detection of short HIV sequences on chitosan/Fe3O4 nanoparticle based screen printed electrodes

Tran, Lam Dai; Nguyen, Binh Hai; Hieu, Nguyen Van; Tran, Hoang Vinh; Nguyen, Huy H.; Nguyen, Phuc

doi:10.1016/j.msec.2010.11.007

Cited by 81 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For biomedical applications, Fe 3 O 4 NPs are often treated with surface modification in order to increase their chemical stability and improve their biocompatibility. A variety of materials have been investigated for the modification of Fe 3 O 4 NPs: precious metals [13], silica [14], carbon [15], biopolymers [16][17][18], and CS. Among these materials, CS is a partially acetylated glucosamine biopolymer with many useful features such as hydrophilicity, biocompatibility, and biodegradability.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and comparative gas-sensing properties of Fe2O3 prepared from Fe3O4 and Fe3O4-chitosan

Cuong

Hòa

Khiếu

et al. 2012

Journal of Alloys and Compounds

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and comparative gas-sensing properties of Fe2O3 prepared from Fe3O4 and Fe3O4-chitosan

Cuong

Hòa

Khiếu

et al. 2012

Journal of Alloys and Compounds

Self Cite

View full text Add to dashboard Cite

“…Studies have been conducted with methylene blue as an indicator of hybridization due to its affinity for the DNA molecule. These links can occur by electrostatic interaction with the negative charges of phosphate groups present in nucleic acids or by the interaction between guanine bases mainly on single‐stranded DNA . Usually, chemical compounds such as methylene blue , ferrocene, and polyaniline have affinities for some bases present in the DNA with potential to intercalation with the double helix.…”

Section: Discussionmentioning

confidence: 99%

A Simple HPV 18 Detection Method Based on Ultra Specific Primer Immobilized on Glass Slides

Fidelis

Dutra

Souza

et al. 2013

Clinical Laboratory Analysis

View full text Add to dashboard Cite

This study was carried out to develop a simple and inexpensive method for detection of Human papillomavirus (HPV 18) based on irreversible immobilization of ultra specific primer on silanized glass slides. This method is revealed by Blue Green Loading Dye I (LGC) and compared with conventional polymerase chain reaction (PCR) for endocervical samples. The new method was tested in 40 DNA samples with precancer uterine lesions of women treated in Hospital of Recife PE, Brazil. DNA samples were extracted using Wizard Genomic DNA Purification Kit according to manufacturer's instructions. The samples were tested for HPV 18 by conventional PCR (PCRc) and the products visualized on 1.2% agarose gel, with LGC under UV 260 nm. After that the positive and negative samples to HPV 18 were tested by immobilization method and the results visualized with LGC under UV 260 nm. Both PCRc and immobilization method showed high degree of correlation (95%), whereas comparison between PCRc and immobilization method showed good correlation (100%). PCRc is widely known for detection of HPV because of its high sensitivity and efficiency, but due to high cost it is not yet standardized for use in public health laboratories. In our study, the single-stranded DNA immobilized method on a glass slide was effective in screening for HPV revealed by Blue Green and may be an alternative method for diagnosis of HPV once it offers a fast, and easy handling.

show abstract

“…Thus, a rapid method of detecting the virus before it can spread further is necessary. A study addressing this need was carried out by Lam Dai Tran et al [ 22 ], who constructed a CS/Fe 3 O 4 nanocomposite platform to detect HIV-1 using an electrochemical method. This platform had a low detection limit (up to 50 pM) and good reproducibility.…”

Section: Screen-printed Electrodes To Detect Pathogensmentioning

confidence: 99%

Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Mincu

Lazăr

Stan³

et al. 2020

Diagnostics

View full text Add to dashboard Cite

Due to rapidly spreading infectious diseases and the high incidence of other diseases such as cancer or metabolic syndrome, there is a continuous need for the development of rapid and accurate diagnosis methods. Screen-printed electrodes-based biosensors have been reported to offer reliable results, with high sensitivity and selectivity and, in some cases, low detection limits. There are a series of materials (carbon, gold, platinum, etc.) used for the manufacturing of working electrodes. Each version comes with advantages, as well as challenges for their functionalization. Thus, the aim is to review the most promising biosensors developed using screen-printed electrodes for the detection/quantification of proteins, biomarkers, or pathogenic microorganisms.

show abstract

Electrochemical detection of short HIV sequences on chitosan/Fe3O4 nanoparticle based screen printed electrodes

Cited by 81 publications

References 26 publications

Synthesis, characterization, and comparative gas-sensing properties of Fe2O3 prepared from Fe3O4 and Fe3O4-chitosan

Synthesis, characterization, and comparative gas-sensing properties of Fe2O3 prepared from Fe3O4 and Fe3O4-chitosan

A Simple HPV 18 Detection Method Based on Ultra Specific Primer Immobilized on Glass Slides

Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Contact Info

Product

Resources

About