Electrochemical devices for cholesterol detection

Domínguez‐Renedo, Olga; Navarro, Ana M.; Alonso‐Lomillo, M. Asunción

doi:10.1016/j.jpba.2022.115195

Cited by 11 publications

(9 citation statements)

References 132 publications

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“…highlight the crucial role of cholesterol as a biomarker for diseases like heart conditions and arteriosclerosis, emphasizing the significance of electrochemical sensors and biosensors in enabling early diagnosis. The review particularly focuses on the advancements in both enzymatic and non‐enzymatic electrochemical systems, emphasizing the integration of lab‐on‐a‐chip devices and exploring different electrode modification strategies using various functional materials [22] . Yadav et al.…”

Section: Introductionmentioning

confidence: 99%

“…The review particularly focuses on the advancements in both enzymatic and non-enzymatic electrochemical systems, emphasizing the integration of lab-on-a-chip devices and exploring different electrode modification strategies using various functional materials. [22] Yadav et al thoroughly explore the utility of functional nanomaterials, emphasizing their crucial contribution in healthcare, specifically focusing on their role in advancing cholesterol biosensor technology. Emphasizing the significant progress made in utilizing nanomaterials for electrochemical cholesterol sensing, the review presents the criticality of these biosensors in clinical cardiovascular disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

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Herein we present a practical, disposable, enzyme‐based electrochemical biosensor to revolutionize a rapid and precise measurement of cholesterol level in saliva. To create a biosensor that can measure cholesterol levels, cholesterol oxidase (ChOx) was immobilized on disposable pencil graphite electrode (PGE) via EDC/NHS chemistry. This ChOx‐PGE biosensor has been characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS). Various critical experimental parameters, including nafion concentration, enzyme concentration, enzyme‐cholesterol interaction time, were optimized ensuring consistency and precision in our results and maintained constant throughout the experiments. Under optimum conditions, the detection limit was found to be 62 μM and the sensitivity of the sensor was calculated as 1112.8 Ohm mM−1 cm−2. The potential interferences such as glucose, ascorbic acid, uric acid, cysteine was selected to test the selectivity and no significant response was observed. Finally, the proposed biosensor was effectively employed to measure cholesterol level in saliva. This biosensor boasts a rapid response time of just 20 minutes and achieves a remarkable detection limit of 95 μM for salivary cholesterol, surpassing existing techniques. Our assay not only offers an innovative solution for cholesterol monitoring but also opens new horizons in point‐of‐care diagnostics with its disposable and practical design.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

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“…However, they are associated with drawbacks such as high analyses costs, the need for skilled personnel to perform the analyses, high enzyme costs, and susceptibility to denaturation. Therefore, more cost‐effective alternative non‐enzymatic electrochemical methods and sensors based on synthetic molecules are being developed for the determination of cholesterol in laboratory settings [8,9] . Metal‐containing materials, including oxides, [10] hydroxides [11] and nanomaterials, [12] are commonly employed as sensitive elements in such methods.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, more cost-effective alternative non-enzymatic electrochemical methods and sensors based on synthetic molecules are being developed for the determination of cholesterol in laboratory settings. [8,9] Metal-containing materials, including oxides, [10] hydroxides [11] and nanomaterials, [12] are commonly employed as sensitive elements in such methods. These materials replace enzymes and enable high signal sensitivity with rapid analysis.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of (3‐Thienyl)‐Substituted 2,2’‐Bipyridines and Their Application as Activators of Platinum Electrode Catalytic Activity Towards Cholesterol Electrooxidation

Okhokhonin.,

Stepanova,

Tsmokaluk

et al. 2024

ChemistrySelect

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Several new derivatives of 2,2′‐bipyridines with a spacer group separated from the 2,2‐bipyridine fragment, namely, an ethynyl fragment, were obtained using the Sonagashira cross‐coupling reaction. The resulting derivatives were physically adsorbed on platinum electrode and were then used as activators of the catalytic activity of a platinum electrode in the reaction of cholesterol electrooxidation in an acetonitrile medium. Sensitivity, limit of detection and detectable concentration range were calculated, and the best results was established as 6.2±0.6 μA/mM, 0.08 mM and 0–0.3 mM, respectively. These results show that the prepared compounds adsorbed on platinum electrode are perspective systems for developing of new enzymeless cholesterol sensor based on them.

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“…Conventional spectrophotometry techniques can be influenced by other related chemicals. Similarly, gas chromatography-mass spectrometry [6], and HPLC [7] involve high instrument costs, skilled operators, chemical solvents, difficult sample preparation, and extended analysis times [8]. Electrochemical sensors [9], on the other hand, are becoming more relevant as bioanalytical sensing instruments for identifying CH and offer a range of benefits, including low cost, rapid testing times, portability, miniaturization, and high sensitivity [10].…”

Section: Introductionmentioning

confidence: 99%

Tin oxide-polyaniline nanocomposite modified nickel foam for highly selective and sensitive detection of cholesterol in simulated blood serum samples

Singh¹,

Sreekumar²,

Badhulika³

2023

Nanotechnology

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Cholesterol (CH) is a vital diagnostic marker for a variety of diseases, making its detection crucial in biological applications including clinical practice. In this work, we report the synthesis of tin oxide-polyaniline nanocomposite-modified nickel foam (SnO2-PANI/NF) for non-enzymatic detection of CH in simulated human blood serum. SnO2 was synthesized via the hydrothermal method, followed by the synthesis of SnO2-PANI nanocomposite through in situ chemical polymerization of aniline using ammonium persulfate as the oxidizing agent. Morphological studies display agglomerated SnO2-PANI, which possess diameters ranging from an average particle size of ∼50 to ∼500 nm, and the XRD analysis revealed the tetragonal structure of the SnO2-PANI nanocomposite. Optimization studies demonstrating the effect of pH and weight percentage are performed to improve the electrocatalytic performance of the sensor. The non-enzymatic SnO2-PANI/NF sensor exhibits a linear range of 1–100 μM with a sensitivity of 300 μA μM−1/cm−2 towards CH sensing and a low limit of detection of 0.25 μM (=3 S m−1). SnO2-PANI/NF facilitates the electrooxidation of CH to form cholestenone by accepting electrons generated during the reaction and transferring them to the nickel foam electrode via Fe (III)/Fe (IV) conversion, resulting in an increased electrochemical current response. The SnO2-PANI/NF sensor demonstrated excellent selectivity against interfering species such as Na+, Cl−, K+, glucose, ascorbic acid, and SO4 2−. The sensor successfully determined the concentration of CH in simulated blood serum samples, demonstrating SnO2-PANI as a potential platform for a variety of electrochemical-based bioanalytical applications.

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Electrochemical devices for cholesterol detection

Cited by 11 publications

References 132 publications

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Synthesis of (3‐Thienyl)‐Substituted 2,2’‐Bipyridines and Their Application as Activators of Platinum Electrode Catalytic Activity Towards Cholesterol Electrooxidation

Tin oxide-polyaniline nanocomposite modified nickel foam for highly selective and sensitive detection of cholesterol in simulated blood serum samples

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