Electrochemical displacement sensor based on ferrocene boronic acid tracer and immobilized glycan for saccharide binding proteins and E. coli

Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification

“…As a result, the glucosyl group was covalently modified on the hydroxymethyl of target DNA. It is well known that boronic acid can covalently bind with 1,2-or 1,3-diol group of the carbohydrates (such as glucose) to form esters in alkaline solution, thus boronic acid and its derivatives (such as 3-aminophenylboronic acid, 4-aminophenylboronic acid, 4-mercaptophenylboronic acid) have been widely applied in electrochemical sensor for immobilizing glycoprotein, antibody and enzymes [27][28][29][30][31][32]. In this work, we selected PDBA as bridging agent and ALP capturing reagent.…”

Recent Progress in Lectin-Based Biosensors

“…The lower detection limits of the sensor were 25 and 50 cells/mL in the electrochemical and QCM detections, respectively. Ferrocene-tagged boroxanol pentamer (Fc-pentaBZE) was synthesized by Scheller and coworkers for the development of electrochemical displacement sensors for E. coli [85]. Fc-pentaBZE was immobilized onto a mannose monolayer-coated electrode as a redox-active marker.…”

Enhanced detection of saccharide using redox capacitor as an electrochemical indicator via a redox-cycling and its molecular logic behavior

“…As a result, much attention has been paid to the development of saccharide sensors based on PBA and its derivatives as recognition components [1,2]. The artificial receptors by appending chromophores [3,4], fluorophores [5,6] and electro-active groups [7][8][9][10][11] covalently associated with PBA have been widely used for recognition and detection of saccharide and other biologically important agents (e.g., dopamine [12,13], sialic acid [14], NAD(P)+/NAD(P)H [15], glycoproteins [16,17], bacteria [18,19] and cell [20]). …”