Electrochemical DNA Sensors with Layered Polyaniline—DNA Coating for Detection of Specific DNA Interactions

Kulikova, Tatjana; Porfireva, Anna; Evtugyn, Gennady; Hianik, Tibor

doi:10.3390/s19030469

Cited by 29 publications

(18 citation statements)

References 50 publications

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“…The variation of the charge transfer resistance averaged for six individual DNA sensors did not exceed 6%. This coincides well with the results obtained earlier for the polyaniline based DNA sensors with impedimetric and voltammetric signals [3,49].…”

Section: Determination Of Doxorubicin With Dna Sensor Based On Copolysupporting

confidence: 92%

Electrochemical DNA Sensor Based on the Copolymer of Proflavine and Azure B for Doxorubicin Determination

Porfireva

Evtugyn

2020

Nanomaterials

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A DNA sensor has been developed for the determination of doxorubicin by consecutive electropolymerization of an equimolar mixture of Azure B and proflavine and adsorption of native DNA from salmon sperm on a polymer film. Electrochemical investigation showed a difference in the behavior of individual drugs polymerized and their mixture. The use of the copolymer offered some advantages, i.e., a higher roughness of the surface, a wider range of the pH sensitivity of the response, a denser and more robust film, etc. The formation of the polymer film and its redox properties were studied using scanning electron microscopy and electrochemical impedance spectroscopy. For the doxorubicin determination, its solution was mixed with DNA and applied on the polymer surface. After that, charge transfer resistance was assessed in the presence of [Fe(CN)6]3−/4− as the redox probe. Its value regularly grew with the doxorubicin concentration in the range from 0.03 to 10 nM (limit of detection 0.01 nM). The DNA sensor was tested on the doxorubicin preparations and spiked samples mimicking blood serum. The recovery was found to be 98–106%. The DNA sensor developed can find application for the determination of drug residues in blood and for the pharmacokinetics studies.

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Section: Determination Of Doxorubicin With Dna Sensor Based On Copolysupporting

confidence: 92%

Electrochemical DNA Sensor Based on the Copolymer of Proflavine and Azure B for Doxorubicin Determination

Porfireva

Evtugyn

2020

Nanomaterials

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“…To protect the native structure of the aptamer it was proposed to cover the aptamer with additional PANI layer obtained in the same conditions as underlying polymer support. A similar approach showed high efficiency for determination of specific interactions between the native DNA and anthracycline drug . The number of potential cycling chosen corresponded to minimal value providing formation of a continuous film.…”

Section: Resultsmentioning

confidence: 99%

“…Voltammetric measurements were performed in direct current mode using AUTOLAB PGSTAT 302 N (Metrohm Autolab b.v., The Netherlands) at 23±2 °C in 5 mL working cell. Working electrode was home‐made GCE produced accordingly to . Double‐junction Ag/AgCl electrode (CH Instruments, code CHI111) was used as reference electrode and Pt wire as counter electrode.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have shown capsulation of native DNA molecules between two PANI layers obtained by electropolymerization on glassy carbon electrode (GCE) . Separation of the electropolymerization and DNA deposition made it possible to overcome the denaturing of biological receptor due to less acidic conditions of its precipitation onto polyaniline.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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Mycotoxins are highly toxic metabolites of some fungi that frequently contaminate water, food and feed and hence cause several human and animal diseases. In this work, a new approach to the fast and reliable determination of aflatoxin M1 (AFM1) in water and milk has been proposed with reagent free protocol of signal measurement. For this purpose, DNA aptamer selective to AFM1 was entrapped between two thin layers of polyaniline (PANI) electrodeposited on glassy carbon electrode. The incubation of the aptasensor in the AFM1 solution results in remarkable decrease of the PANI intrinsic activity monitored by direct current voltammetry or electrochemical impedance spectroscopy. Appropriate calibration curves were linear in the range from 3 to 90 ng/L with limit of detection (LOD) 1–5 ng/L depending on the measurement mode. Mechanism of signal generation involves shielding electrostatic interactions between the PANI and aptamer in the surface layer and variation of its redox activity attributed to the emeraldine form of PANI. Selectivity of the response was proved by similar experiments with aflatoxin B1 and ochratoxin A and by comparison of the results with those obtained with non‐specific aptamer in the sensing layer. Simple protocol for milk pretreatment has been proposed for reliable detection of AFM1 on the level of its threshold limited values (20 ng/L).

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“…After approval for medical application in 1974, it was criticized for rather high cardiotoxicity and a narrow gap between therapeutic and toxic dozen [47]. Recently we have shown that introduction of DNA in the redox active polymers can be used for sensitive determination of intercalating drugs by their influence on the redox activity of carrier [48]. Polyaniline electropolymerized in the presence of DNA changed its redox activity after treatment with doxorubicin.…”

Section: Resultsmentioning

confidence: 99%

DNA-Polylactide Modified Biosensor for Electrochemical Determination of the DNA-Drugs and Aptamer-Aflatoxin M1 Interactions

Stepanova

Smolko

Gorbatchuk

et al. 2019

Sensors

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DNA sensors were assembled by consecutive deposition of thiacalix[4]arenes bearing oligolactic fragments, poly(ethylene imine), and DNA onto the glassy carbon electrode. The assembling of the layers was monitored with scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The configuration of the thiacalix[4]arene core determined self-assembling of the polymeric species to the nano/micro particles with a size of 70–350 nm. Depending on the granulation, the coatings show the accumulation of a variety of DNA quantities, charges, and internal pore volumes. These parameters were used to optimize the DNA sensors based on these coatings. Thus, doxorubicin was determined to have limits of detection of 0.01 nM (cone configuration), 0.05 nM (partial cone configuration), and 0.10 nM (1,3-alternate configuration of the macrocycle core). Substitution of native DNA with aptamer specific to aflatoxin M1 resulted in the detection of the toxin in the range of 20 to 200 ng/L (limit of detection 5 ng/L). The aptasensor was tested in spiked milk samples and showed a recovery of 80 and 85% for 20 and 50 ng/L of the aflatoxin M1, respectively.

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Electrochemical DNA Sensors with Layered Polyaniline—DNA Coating for Detection of Specific DNA Interactions

Cited by 29 publications

References 50 publications

Electrochemical DNA Sensor Based on the Copolymer of Proflavine and Azure B for Doxorubicin Determination

Electrochemical DNA Sensor Based on the Copolymer of Proflavine and Azure B for Doxorubicin Determination

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

DNA-Polylactide Modified Biosensor for Electrochemical Determination of the DNA-Drugs and Aptamer-Aflatoxin M1 Interactions

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