Electrochemical Immunoanalysis for Carcinoembryonic Antigen Based on Multilayer Architectures of Gold Nanoparticles and Polycation Biomimetic Interface on Glassy Carbon Electrode

Abstract

This paper describes a layer-by-layer (LBL) self-assembly process of chitosan (CTS) and gold nanoparticles (Au) on the pretreated negatively charged glassy carbon (GC) electrode to fabricate electrochemistry immunosensor with a nontoxic biomimetic interface, which provided an environment similar to a native system and allowed more freedom in orientation for immobilization of carcinoembryonic antibody (anti-CEA) to monitor carcinoembryonic antigen (CEA). UV-vis spectroscope, atomic force microscopy (AFM), and cyclic voltammetric (CV) measurements were used to follow the multilayer film formation. The performance of the biominetic interface and factors influencing the assay system were investigated in detail. The differential pulse voltammetry (DPV) current response is used for the CEA concentration assay. The dynamic range was from 0.50 to 80.00 ng mL À1 with a detection limit of 0.27 ng mL À1 at 3s. In addition, the experiment results indicate that immobilization described in this proposed method exhibits a good sensitivity, selectivity, and stability.

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

“…7 In the form of low molecular weight or oligomers, it also has a perspective for protection against DNA oxidative damage. 8 In the field of sensors, CHIT was already widely used, for instance amperometric laccase sensing at a glassy carbon electrode, 9 amperometric glucose sensing at a Pt electrode, 10 imunosensing for carcinoembryonic antigen at a glassy carbon electrode with CHIT/Au nanoparticles, 11 hydrogen peroxide sensing at a glassy carbon 12 for the detection of DNA hybridization using CNT/nano zirconium dioxide/CHIT-modified electrodes 13 and for the determination of a colorectal cancer target DNA sequence using a CeO2/CHIT composite matrix. 14 CNTs precipitate in water and in acidic aqueous solutions due to their aggregation caused by hydrophobic interactions and van der Walls forces between the tubes.…”

Control of Tumor Markers Using Nanotechnology

“…Differential pulse voltammetry has also shown its usefulness in the development of direct immunosensors for PSA [32], CEA [33][34][35], and CA 125 [36] determination, using CNTs [32], AuNPs [33,34,36], and magnetic NPs [34,35]. Thus, in a flow injection assay for CEA determination in serum, the surface of a pretreated negatively charged glassy carbon electrode was modified with cationic chitosan and anionic AuNPs, previously to the immobilization of the antibodies [33].…”

“…Thus, in a flow injection assay for CEA determination in serum, the surface of a pretreated negatively charged glassy carbon electrode was modified with cationic chitosan and anionic AuNPs, previously to the immobilization of the antibodies [33]. CEA has been also determined in serum using magnetic Fe 2 O 3 -core/Au-shell composite NPs as affinity support for antibody adsorption [34].…”

Perspectives and potential applications of nanomedicine in breast and prostate cancer

“…Because AuNPs have a large area‐to‐volume ratio that facilitates surface absorption of protein molecules, they are ideal candidates for antibody immobilization. For instance, Yuan et al fabricated a CEA immunosensor via layer‐by‐layer assembly of cationic chitosan and anionic AuNPs on glassy carbon electrodes . The CEA antibody was subsequently immobilized on the electrodes.…”

“…For instance, Yuan et al fabricated a CEA immunosensor via layer-by-layer assembly of cationic chitosan and anionic AuNPs on glassy carbon electrodes. 95 The CEA antibody was subsequently immobilized on the electrodes. In their follow-up study, they reported another CEA immunosensor based on AuNPs/Nano-CaCO 3 /Prussian-blue modified glassy carbon electrodes with a detection limit of 0.1 ng/ml.…”