Electrochemical Immunoassay Detection of Bacillus Anthracis Protective Antigen

Aguilar, Zoraida P.; Narasimhan, Padma Yoga; Wansapura, Chamika M.; Henrichs, A. M. A.; Aday, Julie; Aguilar, Ysmael; Estorninos, Leo

doi:10.1149/1.3005398

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…Immunoassay detection as well as immunohistochemical methods are needed for rapid and accurate detection at the onset of the disease. Quantum dots as labels of antibodies for fluorescent signal generation have become a viable alternative to enzyme and fluorescent dyes [7,8,[14][15][16][17][18][19][20][21][22][23][24]. In this project, we used two kinds of antibodies.…”

Section: Resultsmentioning

confidence: 99%

Breast Cancer Cell Imaging using Semiconductor Quantum Dots

Aguilar

et al. 2009

ECS Trans.

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Breast cancer is the second most common type of cancer, the fifth most common cause of cancer deaths in the US and the world, and the most common cause of cancer death in women. Knowing the accurate stage of the breast cancer is critical for patient survival. Sufficient treatment during the early stages of breast cancer can prevent the spread and transfer of the cancer cells to other parts of the body. But, the cancer cells need to be diagnosed at the early stage in order to start treatment. We report the development of early stage detection of breast cancer cells using quantum dots (QDs) as fluorescent signal generator for detection. The QDs based imaging of breast cancer cells involved two kinds of antibodies: the first was for labeling the cells and the second was for imaging the breast cancer cells. Anti-Her2/neu (1 0 Ab) was used for labeling the captured SK-BR3 cells. The second antibody against antiHer2/neu (2 0 Ab) that was conjugated to QDs (2 0 Ab~QDs) forms the complete assay, SK-BR3 + 1 0 Ab + 2 0 Ab~QDs, to generate the fluorescent cells for imaging. Fluorescent images of the complete assay for SK-BR3 cells were evaluated under a microscope with a UV light source. The preliminary results showed that the breast cancer cell SK-BR3 in the complete assay were successfully observed as fluorescent cells that had brighter signals compared with those labeled with organic dye using similar parameters and the same number of cells. Future directions will involve elimination of non-specific signals, establishment of dynamic range of concentrations detected as well as limits of detection, and multiplex detection of different types cells and/or biomarkers of cancer.

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Section: Resultsmentioning

confidence: 99%

Breast Cancer Cell Imaging using Semiconductor Quantum Dots

Aguilar

et al. 2009

ECS Trans.

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“…At Vegrandis, we develop SAM based microelectrochemical immunoassays inside 50 micron diameter self-contained gold electrode arrays (1). These functionalized sensor arrays are used for the detection of various biomarkers that require detection of low concentrations in order to provide proper treatments (1,2). In the past years, the sensor chips were modified by using 11-mercapto-1-undecanol (MUOL) or 11-mercaptoundecanoic acid (MUA) as the SAM compound.…”

Section: Introductionmentioning

confidence: 99%

Self-Contained Microelectrochemical Assay with Enhanced Capture Surface for Sensitive Detection of Proteins

Wansapura¹

2010

ECS Trans.

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Detection of proteins at very low concentrations is of a major interest in biological, medical, and veterinary applications. Vegrandis has been developing self-contained microelectrode array sensors for the detection of low concentration of proteins. In order to improve the detection limits we have been exploring various compounds that enhance the capture surface of the biosensor. The preliminary results for three types of self-assembling monolayer (SAM) compounds used to modify Vegrandis' microarray sensor chips are reported. These compounds comprised of monothiols with biotin or hydroxyl terminated polyethyleneglycol chains or dithiolated scaffold attached to a central trisubstituted aromatic ring with a carboxylic acid terminated polyethyleneglycol chain. Results for the three SAM systems were compared using a simple mouse IgG model assay. The application of sensor chip towards the development of anti-mortem detection of prion proteins is also discussed.

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