Electrochemical Immunoassay for Detecting Hippuric Acid Based on the Interaction of Osmium-Antigen Conjugate Films with Antibody on Screen Printed Carbon Electrodes

Choi, Young-Bong; Jeon, Won-Yong; Kim, Hyug-Han

doi:10.5012/bkcs.2012.33.5.1485

Cited by 4 publications

(5 citation statements)

References 30 publications

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“…10,11 In this paper, a facile synthetic process enabling the direct coordination of pyridyl-N to pentacyanoferrate for the detection of small organic molecules is reported. 12,13 In particular, two novel strategies are employed to design the homogeneous electrochemical immunoassay system, as follows: First, the synthesized complex between pyridyl-N and pentacyanoferrate was not only an excellent electroactive molecule, but also provided direct conjugation to the antibody for the detection of the target molecules.…”

Section: -4mentioning

confidence: 99%

The Coordination of Pyridyl-N to Pentacyanoferrate for the Electrochemical Detecting Small Organic Molecules

Choi¹,

Jeon²,

Kim³

2013

Bulletin of the Korean Chemical Society

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The coordination of pyridyl-N to pentacyanoferrate for the detection of small organic antigens in solution is presented. The unique contribution of this paper is the direct conjugation of pyridyl-N in small organic antigens to pentacyanoferrate. Pentacyanoferrate is promising as an electrochemical label owing to its good electrochemical properties, which can be utilized to generate an electrical signal in homogeneous electrochemical immunoassays. The facilely synthesized pyridyl-N to pentacyanoferrate was characterized by the electrochemical and spectroscopic methods. Hippuric acid (HA) has been detected competitively on the interaction of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA) to its antibody, with the detection limit of 0.50 µg mL −1. While pentacyanoferrate-based immunoassay is in its simplicity and infancy, the proposed immunoassay offers attractive opportunities for developing pyridyl-N-based the electrochemical detection of small organic antigens in the health care area.

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Section: -4mentioning

confidence: 99%

The Coordination of Pyridyl-N to Pentacyanoferrate for the Electrochemical Detecting Small Organic Molecules

Choi¹,

Jeon²,

Kim³

2013

Bulletin of the Korean Chemical Society

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“…Electrochemical immunoassays can be divided into homogeneous and heterogeneous methods, depending on whether the antibody or antigen needs to be immobilized on the electrode or not. Most reported electrochemical immunoassays have used heterogeneous methods, which usually display higher sensitivity and less interference [ 28 , 29 , 30 ], however, it has been reported that the physical adsorption and covalent conjugation methods used for antibody immobilization on a solid surface can cause random orientation, denaturation, and steric hindrance in the antibodies. The latter reduces the specificity and reproducibility during the immunoassays [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode

Choi

Jeon

Kim

2016

Sensors

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In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl-N ligands, and the redox-active Fe(II/III) centers were bound to Ni(II) ions on the electrode via electrostatic cyanide bridges. The detection was accomplished by the competitive binding of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA, the electron transfer mediator) to the HA antibody on the Ni(II) ions-modified PVI-ITO (Ni-PVI-ITO) electrode. The electrical and physicochemical characterization of the electrode was carried out by cyclic voltammetry, differential pulse voltammetry, field emission scanning electron microscopy, and X-ray photoelectron spectroscopy. At low mediator concentrations, the electrical signals were proportional to the HA concentration between 0.1 µg/mL and 1.0 mg/mL. The same method may be extended to other small organic molecules.

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“…Compared to our previous papers, the benefits of the present paper include (1) the easy immobilization of Ni ions onto the electrode; (2) the prompt synthesis of ferrate antigen; (3) less interference due to the electrostatic interaction between Ni(II) and ferrate; and (4) a relatively low cost [ 29 – 31 ]. In this study, we used electrochemical techniques to form organic films containing imidazole rings on the electrodes ( Figure 1 (1)).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous Electrochemical Immunoassay of Hippuric Acid on the Electrodeposited Organic Films

Choi

Kim

et al. 2014

Sensors

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By directly coordinating hippuric acid (HA) to the ferrate (Fe) as an electron transfer mediator, we synthesized a Fe-HA complex, which shows a good electrochemical signal and thus enables the electrochemical immunoanalysis for HA. We electrodeposited organic films containing imidazole groups on the electrode surface and then bonded Ni ion (positive charge) to induce immobilization of Fe-HA (negative charge) through the electrostatic interaction. The heterogeneous competitive immunoassay system relies on the interaction between immobilized Fe-HA antigen conjugate and free HA antigen to its antibody (anti-HA). The electric signal becomes weaker due to the hindered electron transfer reaction when a large-sized HA antibody is bound onto the Fe-HA. However, in the presence of HA, the electric signal increases because free HA competitively reacts with the HA antibody prior to actual reaction and thus prevents the HA antibody from interacting with Fe-HA at the electrode surface. This competition reaction enabled an electrochemical quantitative analysis of HA concentration with a detection limit of 0.5 μg mL−1, and thus allowed us to develop a simple and rapid electrochemical immunosensor.

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Electrochemical Immunoassay for Detecting Hippuric Acid Based on the Interaction of Osmium-Antigen Conjugate Films with Antibody on Screen Printed Carbon Electrodes

Cited by 4 publications

References 30 publications

The Coordination of Pyridyl-N to Pentacyanoferrate for the Electrochemical Detecting Small Organic Molecules

The Coordination of Pyridyl-N to Pentacyanoferrate for the Electrochemical Detecting Small Organic Molecules

A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode

Heterogeneous Electrochemical Immunoassay of Hippuric Acid on the Electrodeposited Organic Films

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