Electrochemical immunoassay for serum parathyroid hormone using screen-printed carbon electrode and magnetic beads

Malla, Pravanjan; Liao, Hao-Ping; Liu, Chi-Hsien; Wu, Wei‐Chi

doi:10.1016/j.jelechem.2021.115463

Cited by 16 publications

(5 citation statements)

References 39 publications

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“…Few articles have reported the use of EIS with functionalized MBs. However, the ability of this technique to investigate the change in resistance and capacitance of the interface providing insights into the effects of incubation of imunoreagents in the charge transfer process at electrodes modified with MBs have been demonstrated in various articles [ 26 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis

et al. 2022

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Serum level of CCL5 chemokine is considered an emerging biomarker for multiple sclerosis (MS). Due to the lack of specific assays for this disease, the development of a point-of-care test for rapid detection of MS could lead to avoiding diagnostics delays. In this paper, we report the first electrochemical immunoplatform for quantification of the CCL5 biomarker at the clinically required levels, able to discriminate between patients diagnosed with MS and healthy individuals. The immunosensing device involves protein capture from biological samples by complexation with biotinylated specific antibodies immobilized onto neutravidin-functionalized microparticles and sandwich assay with anti-CCL5 antibody and IgG labelled with horseradish peroxidase (HRP) for the enzyme-catalyzed amperometric detection of H2O2 using hydroquinone (HQ) as the redox mediator. The method shows excellent analytical performance for clinical application with a wide linear range of concentrations (0.1–300 ng·mL−1 CCL5, R2 = 0.998) and a low detection limit (40 pg·mL−1 CCL5). The biosensing platform was applied to the determination of the CCL5 endogenous content in 100-fold diluted sera both from healthy individuals and patients diagnosed with MS, with no further sample treatment in just two hours. The results were successfully compared with those obtained by the ELISA methodology.

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Section: Resultsmentioning

confidence: 99%

Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis

et al. 2022

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“…The primary variables associated with SWV are prepotential, start potential, final potential, time at pre-potential, pulse period, pulse amplitude, pulse frequency, and potential step. SWV is considered to possess a superior sensitivity [49]. The utilization of SWV is deemed highly suitable for the electroanalysis of species that have been adsorbed onto the electrode surface [50].…”

Section: Electrochemical Methods For the Detection Of Hormonesmentioning

confidence: 99%

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Haroon,

Stine

2023

Coatings

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Hormones regulate several physiological processes in living organisms, and their detection requires accuracy and sensitivity. Recent advances in nanostructured electrodes for the electrochemical detection of hormones are described. Nanostructured electrodes’ high surface area, electrocatalytic activity, and sensitivity make them a strong hormone detection platform. This paper covers nanostructured electrode design and production using MOFs, zeolites, carbon nanotubes, metal nanoparticles, and 2D materials such as TMDs, Mxenes, graphene, and conducting polymers onto electrodes surfaces that have been used to confer distinct characteristics for the purpose of electrochemical hormone detection. The use of aptamers for hormone recognition is producing especially promising results, as is the use of carbon-based nanomaterials in composite electrodes. These materials are optimized for hormone detection, allowing trace-level quantification. Various electrochemical techniques such as SWV, CV, DPV, EIS, and amperometry are reviewed in depth for hormone detection, showing the ability for quick, selective, and quantitative evaluation. We also discuss hormone immobilization on nanostructured electrodes to improve detection stability and specificity. We focus on real-time monitoring and tailored healthcare with nanostructured electrode-based hormone detection in clinical diagnostics, wearable devices, and point-of-care testing. These nanostructured electrode-based assays are useful for endocrinology research and hormone-related disease diagnostics due to their sensitivity, selectivity, and repeatability. We conclude with nanotechnology–microfluidics integration and tiny portable hormone-detection devices. Nanostructured electrodes can improve hormone regulation and healthcare by facilitating early disease diagnosis and customized therapy.

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“…, through pH, click chemistry or covalent immobilization via SAM assembly (EDC/NHS), using nanobodies (shortened version of an antibody only including the active site), nanoparticles (AuNPs, IrOx etc.) and magnetic beads for increased surface area and conductivity 15 , 16 , or decreasing the electrode size into the micrometer realm 17 . Importantly however, all these modifications exponentially increase the production cost and manufacturing complexity of these sensors.…”

Section: Introductionmentioning

confidence: 99%

A plug-and-play, easy-to-manufacture fluidic accessory to significantly enhance the sensitivity of electrochemical immunoassays

Dobrea,

Hall,

Milne

et al. 2024

Sci Rep

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Earlier access to patients’ biomarker status could transform disease management. However, gold-standard techniques such as enzyme-linked immunosorbent assays (ELISAs) are typically not deployed at the point-of-care due to their cumbersome instrumentation and complexity. Electrochemical immunosensors can be disruptive in this sector with their small size and lower cost but, without further modifications, the performance of these sensors in complex media (e.g., blood) has been limited. This paper presents a low-cost fluidic accessory fabricated using widely accessible materials and processes for boosting sensor sensitivity through confinement of the detection media next to the electrode surface. Liquid confinement first highlighted a spontaneous reaction between the pseudoreference electrode and ELISA detection substrate 3,3’,5,5’-tetramethylbenzidine (TMB) that decreases the amount of oxTMB available for detection. Different strategies are investigated to limit this and maximize reliability. Next, flow cell integration during the signal amplification step of sensor preparation was shown to substantially enhance the detection of cytokine interleukin-6 (IL-6) with the best sensitivity boost recorded for fresh human plasma (x7 increase compared to x5.8 in purified serum and x5.5 in PBS). The flow cell requires no specialized equipment and can be seamlessly integrated with commercial sensors, making an ideal companion for electrochemical signal enhancement.

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Electrochemical immunoassay for serum parathyroid hormone using screen-printed carbon electrode and magnetic beads

Cited by 16 publications

References 39 publications

Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis

Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis

Electrochemical Detection of Hormones Using Nanostructured Electrodes

A plug-and-play, easy-to-manufacture fluidic accessory to significantly enhance the sensitivity of electrochemical immunoassays

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