Electrochemical Immunosensor for Carcinoembryonic Antigen Detection Based on Mo–Mn₃O₄/MWCNTs/Chits Nanocomposite Modified ITO Electrode

Abstract

A novel electrochemical immunosensor for determination of carcinoembryonic antigen (CEA) in human serum was fabricated by depositing Mo-Mn 3 O 4 /MWCNTs/Chits nanocomposite onto an indium-tin oxide (ITO) electrode. Mo-doped Mn 3 O 4 (MMO) was synthesized by sol-gel method and the presence of molybdenum improved its electrochemical properties. The MMO/ MWCNTs/Chits nanocomposite could accelerate the electron transfer rate and enlarge the surface area to capture a large number of Carcinoembryonic Antigen (CEA). The factors in°uencing the performance of the immunosensor were investigated, such as incubation time, incubation temperature and pH. Under optimal conditions, the electrochemical immunosensor could detect CEA in a linear range from 0.1 ng Á mL À1 to 125 ng Á mL À1 with a detection limit of 4.9 pg Á mL À1 (S=N ¼ 3). In addition, it exhibited high sensitivity and acceptable stability on a promising immobilization platform for signal ampli¯cation, which could be extended to other labeled recognition systems. This electrochemical immunosensor may provide potential applications for the clinical diagnosis.

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

“…The sensitivity of the proposed immunosensing approach was compared to some previous electrochemical CEA biosensors based on amplification-free, − and amplification detection approaches. − As depicted in Table , our proposed biosensor exhibited excellent performance regarding its detection limit and analytical range, which either exceeded or matched the performance of the mentioned examples. It is particularly advantageous that our approach does not require labeling or successive steps of isothermal enzymatic signal amplification, thus reducing the potential for errors and simplifying the detection process.…”

A new Bi2MoO6 nano-tremella-based electrochemical immunosensor for the sensitive detection of a carcinoembryonic antigen

“…6 Lung, colorectal and breast cancer can be diagnosed and evaluated early by detecting the concentration of carcinoembryonic antigen (CEA). [7][8][9][10] Therefore, the quick and sensitive detection of tumor markers is signicantly important for the early diagnosis and treatment of cancer.…”

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

“…Electrochemical immunosensor is of increasing interest for its high sensitivity, cost-e®ectiveness, fast response, feasible miniaturization and subsequent portability. 3,4 Sandwich-type electrochemical immunosensor has the advantages of high speci¯city and sensitivity due to the use of a coupling of match antibodies. 5,6 Nevertheless, these types of immunosensors usually need to immobilize enzymes for the signal ampli¯cation.…”