Electrochemical immunosensor for detecting carcinoembryonic antigen using hollow Pt nanospheres-labeled multiple enzyme-linked antibodies as labels for signal amplification

Yang, Hongchuan; Yuan, Ruo; Mao, Li; Su, Huilan; Jiang, Wen

doi:10.1016/j.bej.2011.04.004

Cited by 33 publications

(13 citation statements)

References 40 publications

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“…The different morphologies of metal nanoparticles could affect their properties and suitability for use. For example, Pt nanoparticles with different morphologies such as hollow versus solid Pt nanospheres display different electrochemical characteristics when used as labels in immunosensors and in other applications [ 92 , 93 ]. Cui et al [ 94 ] have developed at a disposable immunosensor array using mesoporous platinum nanoparticles (M-Pt NPs) as nonenzymatic labels.…”

Section: Electrochemical Immunosensors Based On Various Nanomaterimentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

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Section: Electrochemical Immunosensors Based On Various Nanomaterimentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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show abstract

“…The differences in the morphologies of the metal nanoparticles affect their properties and suitability for use in biosensors. For example, platinum (Pt) nanoparticles with different morphologies such as hollow versus solid Pt nanospheres display different electrochemical characteristics when used as labels in immunosensors and in other applications [ 88 ]. The hollow nanospheres have been shown to have better catalytic properties, lower density, higher specific surface area, and lower cost due to utilizing smaller amounts of material during fabrication [ 89 ].…”

Section: Nano- and Biomaterialsmentioning

confidence: 99%

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

2014

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Nanotechnology has played a crucial role in the development of biosensors over the past decade. The development, testing, optimization, and validation of new biosensors has become a highly interdisciplinary effort involving experts in chemistry, biology, physics, engineering, and medicine. The sensitivity, the specificity and the reproducibility of biosensors have improved tremendously as a result of incorporating nanomaterials in their design. In general, nanomaterials-based electrochemical immunosensors amplify the sensitivity by facilitating greater loading of the larger sensing surface with biorecognition molecules as well as improving the electrochemical properties of the transducer. The most common types of nanomaterials and their properties will be described. In addition, the utilization of nanomaterials in immunosensors for biomarker detection will be discussed since these biosensors have enormous potential for a myriad of clinical uses. Electrochemical immunosensors provide a specific and simple analytical alternative as evidenced by their brief analysis times, inexpensive instrumentation, lower assay cost as well as good portability and amenability to miniaturization. The role nanomaterials play in biosensors, their ability to improve detection capabilities in low concentration analytes yielding clinically useful data and their impact on other biosensor performance properties will be discussed. Finally, the most common types of electroanalytical detection methods will be briefly touched upon.

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“…Recently, an interesting work was reported involving this protein investigation using an immunosensor constructed by Pt hollow nanospheres modified with anti-CEA as label for a 3D Au-TiO 2 hybrid platform. [31] The immunoassay exhibited a high sensitivity and a low detection limit compared with conventional label methods. Another way to detect CEA antigen was developed by Gao et al [32] using a label-free voltammetric sensor with chitosan and gold nanoparticles (AuNPs) to immobilize anti-CEA on carbon surface.…”

Section: Metallic Silica and Magnetic Nanoparticlesmentioning

confidence: 99%

Nanomaterials for Advancing the Health Immunosensor

Rodríguez¹,

Trindade²,

Cabral³

et al. 2015

Biosensors - Micro and Nanoscale Applications

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Nanotechnoloμy has exerted a siμniλicant impact in the development oλ biosensors allowinμ more sensible analytical methods. In health applications, the main challenμe oλ the immunoassay is to reach the suitable limit oλ detection, recoμnizinμ diλλerent analytes in complex samples like whole blood, serum, urine, and other bioloμical λluids. Diλλerent nanomaterials, includinμ metallic, silica and maμnetic nanoparticles, quantum dots, carbon nanotubes, and μraphene, have been applied, mainly to improve charμe electron transλer, catalytic activity, amount oλ immobilized biomolecules, low-backμround current, siμnal-to-noise ratio that consequently increase the sensitivity oλ immunosensors. Given the μreat impact oλ nanotechnoloμy, this chapter intends to discuss new aspects oλ nanomaterials relatinμ to immunosensor advancement. Keywords:Immunosensors, immunoassay, nanosensor, nanomaterial . IntroductionA major challenμe λaced by health proμrams is the maintenance and availability oλ diaμnostic tests that are required not only in inpatient or outpatient hospital but also λor an improved epidemioloμical survey. In many cases, the absence oλ laboratory testinμ or delay oλ diaμnosis μenerates neμative economic impacts, resultinμ in unnecessary hospitalization, intercurrence, and in some cases implications on the μlobal liλe quality oλ patients and underreportinμ oλ surveillance. In this context, the development oλ practical, λast, and reliable analytical methods is imperative.Biosensors have been considered one oλ the more attractive analytical methods. They are biodevices capable oλ transλorminμ an interaction with speciλic analytes into an electrical siμnal by a transducer, includinμ a biorecoμnition element. [ ] Pharmaceutical industries and users oλ rapid tests λrom the United States and Europe are unanimous in statinμ that biosensors, mainly those based on point-oλ-care testinμ POCT , or bedside testinμs are a practical technoloμy, reμarded as a short-, medium-, and lonμ-term trend. Amonμ several advantaμes, POCT can provide immediate responses results in λew minutes or in real time , samples do not need to be transported to the analytical phase in situ monitorinμ and require μenerally small volumes oλ samples, and the users can be skilled or unskilled and present better costeλλective analyses compared with conventional technoloμies used in clinical diaμnostic userλriendly technoloμy . One oλ the most widely useλul POCT is the μlucometer, which measures μlucose levels with accuracy by requirinμ a sinμle drop oλ blood. The rapid μlucose measurement is very important in trials to avoid serious adverse eλλects stemminμ λrom diabetes, includinμ seizures, coma, or even death. Worldwide, some diabetic outpatients have been beneλited by POCTs.Althouμh there is a μreat promisinμ market dedicated to health λor the detection oλ diseases and therapeutic monitorinμ, biosensors are not yet entirely broadcast, especially those devoted to nonenzymatic reactions, i.e., biosensors based on the aλλinity between antiμen antibo...

show abstract

Electrochemical immunosensor for detecting carcinoembryonic antigen using hollow Pt nanospheres-labeled multiple enzyme-linked antibodies as labels for signal amplification

Cited by 33 publications

References 40 publications

Nanomaterials for Electrochemical Immunosensing

Nanomaterials for Electrochemical Immunosensing

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

Nanomaterials for Advancing the Health Immunosensor

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