Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers

Chikkaveeraiah, Bhaskara V.; Bhirde, Ashwinkumar; Morgan, Nicole Y.; Eden, Henry S.; Chen, Xiaoyuan

doi:10.1021/nn3023969

Cited by 636 publications

(361 citation statements)

References 105 publications

(222 reference statements)

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“…Because the performance of the uMED and the variety of important pulse sequences that it can perform are comparable to the commercial comparison, the uMED can, in principle, be used in place of an expensive (US$1,000-50,000) bench-top potentiostat in other applications that use these common electrochemical methods. For example, the uMED could use amperometry to detect metabolites (33), proteins, or other biomolecules, or voltammetry to perform immunoassays in which an antibody is labeled with an enzyme (e.g., electrochemical ELISA), metal nanoparticle, or quantum dot (2,24). The vast and fast-growing field of ISEs also provides many opportunities to couple new electrodes with the uMED to measure diverse analytes by potentiometry (34), such as blood urea nitrogen and creatinine (35), which are biomarkers for kidney function.…”

Section: Discussionmentioning

confidence: 99%

“…electrochemistry | mHealth | point-of-care diagnostics | low-cost potentiostat | telemedicine E lectrochemistry provides a broad array of quantitative methods for detecting important analytes (e.g., proteins, nucleic acids, metabolites, metals) for personal and public health, clinical analysis, food and water quality, and environmental monitoring (1,2). Although useful in a variety of settings, these methodswith the important exception of blood glucose meters (3,4)-are generally limited to well-resourced laboratories run by skilled personnel.…”

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confidence: 99%

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Universal mobile electrochemical detector designed for use in resource-limited applications

Nemiroski¹,

Christodouleas²,

Hennek³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

271

192

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This paper describes an inexpensive, handheld device that couples the most common forms of electrochemical analysis directly to "the cloud" using any mobile phone, for use in resource-limited settings. The device is designed to operate with a wide range of electrode formats, performs on-board mixing of samples by vibration, and transmits data over voice using audio-an approach that guarantees broad compatibility with any available mobile phone (from low-end phones to smartphones) or cellular network (second, third, and fourth generation). The electrochemical methods that we demonstrate enable quantitative, broadly applicable, and inexpensive sensing with flexibility based on a wide variety of important electroanalytical techniques (chronoamperometry, cyclic voltammetry, differential pulse voltammetry, square wave voltammetry, and potentiometry), each with different uses. Four applications demonstrate the analytical performance of the device: these involve the detection of (i) glucose in the blood for personal health, (ii) trace heavy metals (lead, cadmium, and zinc) in water for in-field environmental monitoring, (iii) sodium in urine for clinical analysis, and (iv) a malarial antigen (Plasmodium falciparum histidine-rich protein 2) for clinical research. The combination of these electrochemical capabilities in an affordable, handheld format that is compatible with any mobile phone or network worldwide guarantees that sophisticated diagnostic testing can be performed by users with a broad spectrum of needs, resources, and levels of technical expertise.electrochemistry | mHealth | point-of-care diagnostics | low-cost potentiostat | telemedicine E lectrochemistry provides a broad array of quantitative methods for detecting important analytes (e.g., proteins, nucleic acids, metabolites, metals) for personal and public health, clinical analysis, food and water quality, and environmental monitoring (1, 2). Although useful in a variety of settings, these methodswith the important exception of blood glucose meters (3, 4)-are generally limited to well-resourced laboratories run by skilled personnel. If simplified and made inexpensive, however, these versatile methods could become broadly applicable tools in the hands of healthcare workers, clinicians, farmers, and military personnel who need accurate and quantitative results in the field, especially in resource-limited settings. Furthermore, if results of testing were directly linked to "the cloud" through available mobile technology, expertise (and archiving of information) could be geographically decoupled from the site of testing. To enable electrochemical measurements to be performed and communicated in any setting, a useful technology must be (i) able to perform complete electrochemical analyses while remaining low in cost, simple to operate, and as independent of infrastructure as possible; and (ii) compatible with any generation of mobile telecommunications technology, including the low-end phones and 2G networks that continue to dominate communications in much o...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Universal mobile electrochemical detector designed for use in resource-limited applications

Nemiroski¹,

Christodouleas²,

Hennek³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

271

192

View full text Add to dashboard Cite

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“…This paper's section will be devoted to the description of the some innovative electrochemical immunosensors for the detection of clinically important protein biomarkers. In particular, we focus our attention on label-free and no-label free electrochemical immunosensors based on the use of SPEs coupled with gold nanoparticles, carbon nanotubes, graphene, magnetic particles and quantum dots (QDs) as new strategies to improve the analytical performances of the conventional SPE-immunosensors [36].…”

Section: Innovative Electrochemical Immunosensors Based On the Use Ofmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

336

166

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A B S T R A C TThis review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognition elements such as enzymes, antibodies, DNA and aptamers to obtain probes with improved analytical features. Examples of clinical applications are illustrated, together with examples of paper-based electrochemical devices, of multiple detections using arrays of screen printed electrodes, and of the most recent developments in the field of wearable biosensors. Also the use of smartphones as final detectors is briefly depicted.

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“…Developing ultrasensitive, quantitative, and cost‐effective methodologies for the detection of disease‐related low‐abundance biomarkers is becoming an unstoppable trend and should be seen as an exciting challenge 1. Enzyme‐based colorimetric assays are widely recognized as such kind of techniques around the world for clinical detections, thanks to their easy operation, straightforward readout, and good practicality 2.…”

Section: Introductionmentioning

confidence: 99%

A Metal Chelator as a Plasmonic Signal‐Generation Superregulator for Ultrasensitive Colorimetric Bioassays of Disease Biomarkers

et al. 2018

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Enzyme‐based assays have been widely applied in clinical diagnosis for decades. However, the intrinsic limitations of enzymes, such as low operation stability, mediocre sensitivity, and high cost in production and purification, heavily constrain their detection application. Here, an enzyme‐free assay is reported that relies on the strong chelating capability of ethylenediamine tetraacetic acid disodium salt (EDTA•2Na, the chelator) for Au3+ ions, in which the cheap EDTA•2Na labeled by targeting moieties can selectively regulate the growth of plasmonic gold nanoparticles (AuNPs) at the target site subjecting to the concentration of analyte in samples. Independent of ambient temperature and unstable H2O2, EDTA•2Na perform superregulation in AuNPs plasmonic signal generation with distinct tonality and outstanding reliability. Upon integrating with silica nanoparticles as the signal amplifying platform, EDTA•2Na‐regulated bioassay can lead to detection‐sensitivity enhancements exceeding three orders of magnitude in protein detection, compared with the gold‐standard assay. The limit of detection of the HBsAg and alpha fetoprotein (AFP) pushes down to 2.6 × 10−15 and 2.5 × 10−19 g mL−1, respectively. EDTA•2Na‐regulated bioassay is also challenged in the clinical serum sample detection and a good consistency is found with the chemiluminescence immunoassay method in clinics.

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Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers

Cited by 636 publications

References 105 publications

Universal mobile electrochemical detector designed for use in resource-limited applications

Universal mobile electrochemical detector designed for use in resource-limited applications

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

A Metal Chelator as a Plasmonic Signal‐Generation Superregulator for Ultrasensitive Colorimetric Bioassays of Disease Biomarkers

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