Electrochemical Impedance Spectroscopy Study of Concanavalin A Adsorption on Glassy Carbon Electrode: An Analysis of Capacitance Dispersion

Raposo, Diego; Diniz, Flamarion B.

doi:10.1016/j.electacta.2013.12.021

Cited by 7 publications

(4 citation statements)

References 69 publications

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“…The phase angle of the Q element is related to n parameter, where n is related to the dispersion of relaxation time through the surface due to a non-homogeneity in the capacitance. 34 Electron transfer resistance controls the electron transfer kinetics of the redox-probe at the electrode/electrolyte interface. Table 1 shows the equivalent circuit parameters of the fitting curves for the immunosensor preparation followed by antibody interaction with different concentrations of AFB 1 in samples.…”

Section: Aflatoxin B 1 Detectionmentioning

confidence: 99%

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice

Simão¹,

Barbieri²,

Andrade³

et al. 2015

Journal of the Brazilian Chemical Society

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An electrochemical immunosensor for detection of aflatoxin B 1 (AFB 1 ) in food samples was developed. The sensor is composed by cysteine monolayer immobilized on gold electrode surface with subsequent bind to monoclonal antibody (MAb-Cys-modified electrode). The AFB 1 detection was evaluated by cyclic voltammetry and impedance spectroscopy in the frequency range of 100 mHz-100 kHz. Samples of rice were spiked with AFB 1 to evaluate the sensitivity of the sensor. Impedance spectra could be fitted to a Randles equivalent circuit containing a constant phase element. Atomic force microscope (AFM) images showed MAb-AFB 1 complex immobilized across the electrode surface. The electron transfer resistance (R ct ) increase was attributed to a decrease in the charge permeability of the MAb-AFB 1 -Au surface to a redox probe . The surface coverage exhibited a linear relationship as function of toxin concentration and is found to be 0.75 at 30 µg mL −1 . The obtained sensor is a promising candidate for detection of AFB 1 in rice with sensitivity and specificity.

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Section: Aflatoxin B 1 Detectionmentioning

confidence: 99%

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice

Simão¹,

Barbieri²,

Andrade³

et al. 2015

Journal of the Brazilian Chemical Society

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“…However, if electrodes are modified with biochemical molecules, the equivalent capacitance of the interface may not solely be the electric double-layer capacitance, but rather a composite capacitance. To address this issue, researchers have proposed electrochemical capacitance spectroscopy (ECS) to assess the physicochemical characteristics of the electrode interface [16,17].…”

Section: Introductionmentioning

confidence: 99%

Redoxless Electrochemical Capacitance Spectroscopy for Investigating Surfactant Adsorption on Screen-Printed Carbon Electrodes

et al. 2023

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Electrochemical impedance spectroscopy (EIS) is a sensitive analytical method for surface and bulk properties. Classical EIS and derived electrochemical capacitance spectroscopy (ECS) with a redox couple are label-free approaches for biosensor development, but doubts arise regarding interpretability when a redox couple is employed (redox EIS) due to interactions between electroactive probes and interfacial charges or forced potential. Here, we demonstrated redoxless ECS for directly determining surfactant adsorption on screen-printed carbon electrodes (SPCEs), validated through a simulation of equivalent circuits and the electrochemistry of electronic dummy cells. Redoxless ECS provides excellent capacitance plot loci for quantifying the interfacial permittivity of di-electric layers on electrode surfaces. Redoxless ECS was compared with redox EIS/ECS, revealing a favorable discrimination of interfacial capacitances under both low and high SDS coverage on SPCEs and demonstrating potential for probeless (reagentless) sensing. Furthermore, the proposed method was applied in an electrolyte without a redox couple and bare electrodes, obtaining a high performance for the adsorption of surfactants Tween-20, Triton-X100, sodium dodecyl sulfate, and tetrapropylammonium bromide. This approach offers a simple and straightforward means for a semi-quantitative evaluation of small molecule interactions with electrode surfaces. Our proposed approach may serve as a starting point for future probeless (reagentless) and label-free biosensors based on electrochemistry, eliminating disturbance with surface charge properties and minimizing forced potential bias by avoiding redox couples. An unambiguous and quantitative determination of physicochemical properties of biochemically recognizable layers will be relevant for biosensor development.

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“…Interactions of Con A with analytes may be registered in a label-free mode using different experimental techniques, like surface plasmon resonance (SPR) [24,29,30], quartz crystal microbalance [15,[19][20][21]31] or electrochemical impedance on a Con A modified metal electrodes [12,[32][33][34][35]. Biosensors based on the last two techniques are the most widely reported.…”

Section: Introductionmentioning

confidence: 99%

“…Immobilization of Con A may be performed using different assembling techniques like absorption [25,27,32], or chemical grafting via self-assembled monolayers (SAM) on gold [15,21,38]. However, Con A directly adsorbed of a solid surface show low stability [47] and readily desorbs [31].…”

Section: Introductionmentioning

confidence: 99%

Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

Brosel-Oliu

Galyamin

Abramova

et al. 2017

Electrochimica Acta

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An impedimetric sensor based on a three dimensional electrode array modified with concanavalin A (Con A) was used for label-free detection of bacterial endotoxin: lipopolysaccharide (LPS) from Escherichia coli. The transducer permits the detection of the surface charge changes due to interaction of immobilized Con A biorecognition element and LPS of E. coli in test solution. The deposition of Con A on the surface was carried out using the layer-by-layer method with polyethyleneimine (PEI) polycation as an initial layer. The sensor surface characterization by means of electrochemical impedance spectroscopy technique allowed registering variations in superficial resistance provoked by surface charge changes and is demonstrated as an effective method to monitor sensor parameters at each modification step as well as to follow Con A-LPS reaction. In order to prevent nonspecific adsorption of LPS on PEI covered surface different blocking strategies were tested to achieve the specific response between Con A and LPS. Results obtained in this work clearly show that blocking with bovine serum albumin (BSA) is not sufficient to prevent non-specific interactions of PEI and to ensure the selective biorecognition of LPS by Con A. To achieve more efficient PEI blocking a new method was proposed based on consecutive deposition of Con A-glycogen-Con A layers. Sensors modified with PEI-(Con A-Gly)2-Con A multilayers are shown to be highly sensitive, selective and reproducible. Presented biosensor is able to detect bacterial LPS in a very short detection time (20 min) with 1.5 µg/mL limit of detection, which is much lower than reported for other biosensors with Con A. CENTRO NACIONAL DE MICROELECTRÓNICA INSTITUTO DE MICROELECTRÓNICA DE BARCELONA Dear editor, On behalf of the authors I would like to submit a manuscript: " Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration" based on the work carried out in

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Electrochemical Impedance Spectroscopy Study of Concanavalin A Adsorption on Glassy Carbon Electrode: An Analysis of Capacitance Dispersion

Cited by 7 publications

References 69 publications

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice

Redoxless Electrochemical Capacitance Spectroscopy for Investigating Surfactant Adsorption on Screen-Printed Carbon Electrodes

Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

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Electrochemical Impedance Spectroscopy Study of Concanavalin A Adsorption on Glassy Carbon Electrode: An Analysis of Capacitance Dispersion

Cited by 7 publications

References 69 publications

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B1in Rice

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B1in Rice

Redoxless Electrochemical Capacitance Spectroscopy for Investigating Surfactant Adsorption on Screen-Printed Carbon Electrodes

Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

Contact Info

Product

Resources

About

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice

Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B₁in Rice