Electrochemical impedance spectroscopy study of liposome adsorption and rupture on self-assembled monolayer: Effect of surface charge

Fujino, Yusuke; Nakamura, Ryo; Han, Huanwen; Yamashita, Ichiro; Shimizu, Tomohiro; Shingubara, Shoso; Ito, Tetsuo

doi:10.1016/j.jelechem.2020.114572

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…Table 1 summarizes the water contact angle values of the variously functionalized surfaces. The contact angles are in a good agreement with those reported in previous works [ [54] , [55] , [56] , [57] , [58] , [59] ].…”

Section: Resultssupporting

confidence: 91%

Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants

Dobrynin

Polishchuk

Portal

et al. 2022

Materials Today Bio

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Section: Resultssupporting

confidence: 91%

Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants

Dobrynin

Polishchuk

Portal

et al. 2022

Materials Today Bio

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“…Indeed, while we classify both compounds as causing membrane disruption, which is defined as inducing irreversible or reversible changes in the mass and viscoelastic properties of the SLB platform as determined by the QCM-D measurements, the specific nature of membrane disruption caused by each compound is distinct. While the TX-100 interaction with model liposomal and biological membranes has previously been characterized using the QCM-D technique, incomplete membrane solubilization and/or other more complex measurement responses were observed in those past studies [ 37 , 38 , 39 , 40 , 41 ] and our findings demonstrate that the SLB platform combined with QCM-D measurement analysis is able to clearly detect complete membrane solubilization, i.e., full and irreversible removal of the adsorbed lipid bilayer mass from the sensor surface, which occurs on a short time scale, provided that the TX-100 concentration is around its CMC or higher. Determination of the lipid bilayer mass removal was judged by the final QCM-D measurement responses post-washing, which returned to the original baseline values prior to SLB fabrication when there was only buffer solution in the measurement chamber.…”

Section: Resultsmentioning

confidence: 99%

Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

Gooran

Yoon

Jackman

2022

IJMS

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Triton X-100 (TX-100) is a widely used detergent to prevent viral contamination of manufactured biologicals and biopharmaceuticals, and acts by disrupting membrane-enveloped virus particles. However, environmental concerns about ecotoxic byproducts are leading to TX-100 phase out and there is an outstanding need to identify functionally equivalent detergents that can potentially replace TX-100. To date, a few detergent candidates have been identified based on viral inactivation studies, while direct mechanistic comparison of TX-100 and potential replacements from a biophysical interaction perspective is warranted. Herein, we employed a supported lipid bilayer (SLB) platform to comparatively evaluate the membrane-disruptive properties of TX-100 and a potential replacement, Simulsol SL 11W (SL-11W), and identified key mechanistic differences in terms of how the two detergents interact with phospholipid membranes. Quartz crystal microbalance-dissipation (QCM-D) measurements revealed that TX-100 was more potent and induced rapid, irreversible, and complete membrane solubilization, whereas SL-11W caused more gradual, reversible membrane budding and did not induce extensive membrane solubilization. The results further demonstrated that TX-100 and SL-11W both exhibit concentration-dependent interaction behaviors and were only active at or above their respective critical micelle concentration (CMC) values. Collectively, our findings demonstrate that TX-100 and SL-11W have distinct membrane-disruptive effects in terms of potency, mechanism of action, and interaction kinetics, and the SLB platform approach can support the development of biophysical assays to efficiently test potential TX-100 replacements.

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“…This work characterized these membranes and investigated the effect of gramicidin, showing how this channel-forming protein increases the membrane conductance, and how the influence of gramicidin can be reduced with divalent cations, such as when CaCl 2 is used in the buffer solution. More recently, EIS has been utilized to characterize microcavity pore-suspended lipid bilayers for detecting membrane-drug activity [ 205 ], as well to investigate the adsorption and attachment of lipid vesicles on a solid substrate [ 206 ]. Not limited to solid-supported membranes, EIS has been used on networks of membranes formed by adhesive emulsion systems such as a network of DIBs, where the impedance response allows for multiple membrane studies and total network analysis [ 176 , 177 ].…”

Section: Electrophysiological Methods For Characterizing Lipid Membranesmentioning

confidence: 99%

Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology

El-Beyrouthy

Freeman

2021

Membranes

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The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions.

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Electrochemical impedance spectroscopy study of liposome adsorption and rupture on self-assembled monolayer: Effect of surface charge

Cited by 10 publications

References 32 publications

Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants

Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants

Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology

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