Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS)

Abstract

For the purpose of human disease prevention, several methods have been developed, and are still developing, to assess the oxidative stress status (OSS) of individuals. In the present paper, we describe an approach based on electrochemical detection able to evaluate skin oxidative stress status (SOSS) as a PAOT (Pouvoir AntiOxydant Total)-Skin Score®. Normal reference values for the PAOT-Skin Score® were: 0–62.94 (n = 263). Intra- and inter-assay coefficients of variation were, respectively, 12.47 ± 4.29% and 7.0 ± 2.5%. Our technology showed increased skin antioxidant activity following topical application of reduced coeznyme Q10 cream or vitamin C intake as orange juice or supplements. Moreover, we found significant correlations between some blood oxidative stress biomarkers and the PAOT-Skin Score® (γ-tocopherol/α-tocopherol ratio (r = 0.43, p = 0.020); copper (r = −0.42, p = 0.022); copper/zinc ratio (r = −0.49, p = 0.006), and lipid peroxides (r = −0.43, p = 0.002)). In addition to being non–invasive, the present electrochemical methodology is also not expensive, fast, and easy to use.

Oxidative Stress Status in COVID-19 Patients Hospitalized in Intensive Care Unit for Severe Pneumonia. A Pilot Study

“…The determination of the total antioxidant capacity (TAC) has been proposed as a global measure of non-enzymatic antioxidant efficiency despite the problem of inference with uric acid [ 53 , 54 ]. Using an original electrochemical methodology developed by us [ 31 ], we showed that the TAC as evaluated by the PAOT ® score was logically decreased in COVID-19 patients most probably because of their very low levels in vitamin C and GSH. Nevertheless, no correlation could be evidenced between both antioxidants and TAC as also shown in the EPIC Granada–Gipuzkoa study [ 55 ].…”

“…White blood cells and neutrophils were determined by flow fluorocytometry on Sysmex-SN device (Ontario, Canada). The total antioxidant capacity (TAC) of plasma was evaluated by using the PAOT ® (Pouvoir AntiOxydant Total, Institut Européen des Antioxydants, Nancy, France) score as previously described [ 31 ]. Briefly, the measurement was carried out in a reaction medium (1 mL physiological solution at pH ranging from 6.7 to 7.2, temperature 24–27 °C) containing a molecule in a free radical state called mediator (M•).…”

Oxidative Stress Status in COVID-19 Patients Hospitalized in Intensive Care Unit for Severe Pneumonia. A Pilot Study

“…The determination of the total antioxidant capacity (TAC) has been proposed as a global measure of non-enzymatic antioxidant efficiency despite the problem of inference with uric acid [53,54]. Using an original electrochemical methodology developed by us [31], we showed that the TAC as evaluated by the PAOT ® score was logically decreased in COVID-19 patients most probably because of their very low levels in vitamin C and GSH. Nevertheless, no correlation could be evidenced between both antioxidants and TAC as also shown in the EPIC Granada-Gipuzkoa study [55].…”

Blue light protection, part II—Ingredients and performance testing methods

“…An equation is then used to determine the score and determine the overall amount of stress. 48 Recently, a new device was developed by Amorepacific that emits blue light at 456 nm, and can control light intensity and light irradiation time. Additionally, the device can also detect the harmful blue light wavelengths and clinically evaluate blue light blocking performance at those harmful wavelengths.…”