Electrochemical monitoring of ROS generation by anticancer agents: the case of chartreusin

Abstract

Generation of ROS by anticancer agents is monitored using solid state electrochemical techniques.

Singlet Oxygen Generation from Polyaminoglycerol by Spin-Flip-Based Electron Transfer

“…The potential for O 2 •– generation ranges from 0 to −1.0 V (vs NHE), depending on the electron donor. 37 Therefore, the peak at −0.87 V (marked with an asterisk) was consistent with the generation of O 2 •– from hPAG ( Figure 2 a, left). The other new peak at +0.94 V (double asterisks) indicates the conversion of O 2 •– to H 2 O 2 ( Figure 2 a, right).…”

“…Cyclic voltammetry (CV) was used to examine the electron transfer from hPAG to produce O 2 •– using ferrocene as the external standard (+0.63 V vs NHE) (Figures and S15). − To identify the ROS generated through electron transfer, current changes from hPAG and hPG under O 2 or N 2 bubbling were analyzed (Figure a). In contrast to the CV spectrum of hPAG under saturated N 2 conditions, new peaks at +0.94 and −0.87 V (vs NHE) appeared under saturated O 2 conditions under a negative sweep (reduction followed by oxidation).…”

Electrochemical DNA Sensors with Layered Polyaniline—DNA Coating for Detection of Specific DNA Interactions

“…This affects their biochemical functions [5]. Intercalators can also promote oxidative DNA damage via oxidation of guanine residues and formation of 8-oxoguanine, an indicator of oxidative stress [6,7]. For this reason, DNA sensors based on native DNA molecules are considered to be promising for detection of drug residues in biological liquids and for searching more effective and less toxic cytostatic drugs.…”

Bioanalytical chemistry with scanning electrochemical microscopy

“…In particular, the recent development of nanoelectrode fabrication has made it possible to analyze a single cell at higher spatio‐temporal resolutions 67 . Examples include high‐resolution imaging of biological samples, 68–70 measurement of oxygen consumption, 71–80 monitoring of ROS/RNS inside and outside cells, 81–89 and monitoring of exocytosis 90–94 . Because SECM bioanalysis with nanoelectrodes has good sensitivity, signal to noise ratio, and non‐invasive in nature, it is suitable for detecting small amounts of chemical species in a single cell.…”