Electrochemical monitoring of the interaction between anticancer drug and DNA in the presence of antioxidant

Muti, Merve; Muti, Mihrican

doi:10.1016/j.talanta.2017.08.089

Cited by 37 publications

(34 citation statements)

References 31 publications

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“…As can be seen in Figure 2 a, two clear non-overlapping peaks were observed at about +600 and +900 mV, corresponding to the electrochemical oxidations of G and A residues, respectively. The observed electrooxidation potentials were similar to the values reported elsewhere [ 21 , 34 , 43 , 44 , 45 , 46 ]. At the same time, no peaks were found during the potential scan in the absence of dsDNA, that is, for a reference SPE/(P n BMA x - b -PDMAEMA y + MWCNT) construct.…”

Section: Resultssupporting

confidence: 90%

Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

et al. 2020

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We demonstrate the application of amphiphilic ionic poly(n-butylmethacrylate)-block- poly(2-(dimethylamino)ethyl methacrylate) diblock copolymers (PnBMA40-b-PDMAEMA40, PnBMA40-b-PDMAEMA120, PnBMA70-b-PDMAEMA120) for dispersing multiwalled carbon nanotubes (MWCNTs) in aqueous media, a subsequent efficient surface modification of screen-printed electrodes (SPEs), and the application of the modified SPEs for DNA electrochemistry. Stable and fine aqueous dispersions of MWCNTs were obtained with PnBMAx-b-PDMAEMAy diblock copolymers, regardless of the structure of the copolymer and the amount of MWCNTs in the dispersions. The effect of the diblock copolymer structure was important when the dispersions of MWCNTs were deposited as modifying layers on surfaces of SPEs, resulting in considerable increases of the electroactive surface areas and great acceleration of the electron transfer rate. The SPE/(PnBMAx-b-PDMAEMAy + MWCNT) constructs were further exploited for direct electrochemical oxidation of the guanine (G) and adenine (A) residues in a model salmon sperm double-stranded DNA (dsDNA). Two well-defined irreversible oxidation peaks were observed at about +600 and +900 mV, corresponding to the electrochemical oxidation of G and A residues, respectively. A multi-parametric optimization of dsDNA electrochemistry enables one to get the limits of detection (LOD) as low as 5 μg/mL (0.25 μM) and 1 μg/mL (0.05 μM) for G and A residues, respectively. The achieved sensitivity of DNA assay enables quantification of the A and G residues of dsDNA in the presence of human serum and DNA in isolated human leukocytes.

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Section: Resultssupporting

confidence: 90%

Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

et al. 2020

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“…Therefore, it can be assumed that the main oxidation step of IND should occur on the piperazine ring. According to the literature, a nitrogen on the piperazine ring loses an electron to form a cation radical, which, in subsequent steps, loses a proton and an electron to form a quaternary Schiff base [ [25] , [26] , [27] ].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical, spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

Mollarasouli

Dogan‐Topal

Çağlayan

et al. 2020

Journal of Pharmaceutical Analysis

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In this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and deoxyadenosine (dAdo), measured by differential pulse voltammetry, upon incubation with different concentrations of indinavir can be attributed to the binding mode of indinavir to ct-dsDNA. The currents of the dGuo and dAdo peaks decreased linearly with the concentration of indinavir in the range of 1.0–10.0 μg/mL. The limit of detection and limit of quantification for indinavir were 0.29 and 0.98 μg/mL, respectively, based on the dGuo signal, and 0.23 and 0.78 μg/mL, respectively, based on the dAdo signal. To gain further insights into the interaction mechanism between indinavir and ct-dsDNA, spectroscopic measurements and molecular docking simulations were performed. The binding constant (K b ) between indinavir and ct-dsDNA was calculated to be 1.64 × 10 8 M −1 , based on spectrofluorometric measurements. The obtained results can offer insights into the inhibitory activity of indinavir, which could help to broaden its applications. That is, indinavir can be used to inhibit other mechanisms and/or hallmarks of viral diseases.

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“…These nanoparticles provide the researchers with more efficient techniques for interfacing DNA recognition with electrochemical signal transduction. The recent electrochemical drug‐DNA binding studies based on nanomaterials are summarized in Table ; the types of drugs that have been analyzed are anticancer agents antibiotics, antihistaminic, antivirals, nonsteroidal anti‐inflammatory drugs, neurotransmitters, vitamins and retroviral . Upon discovery of the advantages that nanomaterials could bring to analytical science, the first researches regarding to DNA‐drug interaction were done in solution.…”

Section: Nanomaterialsmentioning

confidence: 99%

“…The interaction mechanism of drugs with DNA has been searched thoroughly during the past decades , giving rise to an endless number of findings of undoubted importance, such as a prompt alert to harmful substances, ability to explain most of the biological mechanisms, or provision of important clues in targeted development of novel chemotherapeutics . The process of interaction involves small or large molecules that selectively recognize each other through different types of molecular interactions, i. e., electrostatic, dipole‐dipole, hydrogen bonding, π‐π, and van der Waals interactions.…”

Section: Introductionmentioning

confidence: 99%

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

2019

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The interaction of drugs with DNA has been searched thoroughly giving rise to an endless number of findings of undoubted importance, such as a prompt alert to harmful substances, ability to explain most of the biological mechanisms, or provision of important clues in targeted development of novel chemotherapeutics. The existence of some drugs that induce oxidative damage is an increasing point of concern as they can cause cellular death, aging, and are closely related to the development of many diseases. Because of a direct correlation between the response, strength/ nature of the interaction and the pharmaceutical action of DNA‐targeted drugs, the electrochemical analysis is based on the signals of DNA before and after the interaction with the DNA‐targeted drug. Nowadays, nanoscale materials are used extensively for offering fascinating characteristics that can be used in designing new strategies for drug‐DNA interaction detection. This work presents a review of nanomaterials (NMs) for the study of drug‐nucleic acid interaction. We summarize types of drug‐DNA interactions, electroanalytical techniques for evidencing these interactions and quantification of drug and/or DNA monitoring.

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Electrochemical monitoring of the interaction between anticancer drug and DNA in the presence of antioxidant

Cited by 37 publications

References 31 publications

Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

Electrochemical, spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

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