No abstract

A ltın nanopartiküller (AuNPs) ile zenginleştirilmiş 5-amino-2-mercapto-1,3,4-thidiazole (AMT) modifiye impedimetrik sensörler, Hepatit B virüsüne (HBV) ilişkin dizi seçimli DNA hibridizasyonunun elektrokimyasal olarak izlenmesi için geliştirilmiştir. AMT-AuNP-PGE ler daha fazla miktarda DNA'nın AMT-AuNPs-PGE yüzeyine bağlanması için uygun bir yüzey sağlamış ve daha tekrarlanabilir impedimetrik sinyaller vermiştir. AMT-AuNP ile modifiye edilmiş DNA biyosensörlerin seçimliliği, hedef DNA, veya diğer DNA dizileri; rastgele dizi (NC), mutasyonlu dizi (MM) varlığında test edilmiştir. Tayin sınırı 86 µg/mL olarak hesaplanmıştır. Anahtar KelimelerAltın nanopartiküller, 5-amino-2-mercapto-1,3,4-thidiazole, DNA hibridizasyonu, impedimetrik sensor. A B S T R A C T5 -amino-2-mercapto-1,3,4-thidiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV). AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface. The selectivity of DNA biosensor modified with AMT-AuNP was investigated in the presence of target DNA, or the other DNA sequences; e.g, noncomplementary (NC), or mismatch (MM) DNA sequences. The detection limit was calculated as 0.86 µg/mL.

A novel DNA probe based on caffeic acid modified disposable pencil graphite electrodes were developed for the first time for the electrochemical determination of breast cancer gene sequence (BRCA) hybridization. Amino‐linked BRCA probe highly immobilized onto the caffeic acid modified electrode by means of the interaction between the amino group of BRCA probe and the carboxyl group of caffeic acid compared to the bare electrode. 44 % signal enhancement in guanine oxidation signal was measured by caffeic acid modified electrode. Besides, these probes exhibited high selectivity towards its complementary DNA sequences (target). Hybridization between probe and target (BRCA1) was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The selectivity was also tested in the presence of mixture containing the target and one base mismatch BRCA sequences in the same ratio (1 : 1). It can be said this probe can select its complementary from the mixture.