Electrochemical Motion Tracking of Microorganisms Using a Large‐Scale‐Integration‐Based Amperometric Device

Abstract

Motion tracking of microorganisms is useful to investigate the effects of chemical or physical stimulation on their biological functions. Herein, we describe a novel electrochemical imaging method for motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device. The device consists of 400 electrochemical sensors with a pitch of 250 μm. A convection flow caused by the motion of microorganisms supplies redox species to the sensors and increases their electrochemical responses. Thus, the flow is converted to electrochemical signals, enabling the electrochemical motion tracking of the microorganisms. As a proof of concept, capillary vibration was monitored. Finally, the method was applied to monitoring the motion of Daphnia magna. The motions of these microorganisms were clearly tracked based on the electrochemical oxidation of [Fe(CN) ] and reduction of O .

Electrochemical imaging of cells and tissues

“…2b ). 31 Microchips with 8′192 Pt MEs (∼1′150 dpi) were developed to record the microfluidic flows of solutions with different norepinephrine concentrations within 64 s ( Fig. 2c ).…”

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

“…For example, we developed a large-scale integration (LSI)-based amperometric sensor for electrochemical imaging, which was designated the Bio-LSI device. [57][58][59][60][61][62][63][64] This device was applied to the electrochemical evaluation and real-time monitoring of dopamine release from 3D-cultured PC12 cells 62 and ALP activity of EBs. 58 As shown in Fig.…”

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

“…(11) In addition to these devices, we developed a CMOS-based amperometric device, which we named the Bio-LSI device. (16)(17)(18)(19)(20) The amperometric device was applied to bioassays including cell analysis. For example, we successfully monitored the alkaline phosphatase (ALP) activity in embryonic stem (ES) cells to evaluate the differentiation levels of these cells.…”