Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus

Teengam, Prinjaporn; Siangproh, Weena; Tuantranont, Adisorn; Henry, Charles S.; Vilaivan, Tirayut; Chailapakul, Orawon

doi:10.1016/j.aca.2016.11.071

Cited by 202 publications

(95 citation statements)

References 50 publications

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“…The hybridization between the HPV type16 target and DNA probe was investigated by SWV. The detection limit of HPV type 16 DNA was found at 2.3 nM with a linear range of 10–200 nM .…”

Section: Methodsmentioning

confidence: 97%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

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Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV‐16 and 18, has been a topic of focus by many researchers. In this systematic review, we highlight the route of development of DNA‐based genosensory detection methods for diagnosis of high risk of HPV precancer. Biosensor detection methods of HPV‐16 and 18 was investigated from 1994 to 2018 using several databases including PubMed, Cochrane Library, Scopus, Google Scholar, SID, and Scientific Information Database. Manual search of references of retrieved articles were also performed. A total of 50 studies were reviewed. By analyzing the most recent developed electrochemical biosensors for the identification of HPV, we observed that the sensor platform fabricated by Wang et al. holds the lowest detection limit reported in the literature for the DNA of HPV‐16. Up to this date, optical, electrochemical, and piezoelectric systems are the main transducers used in the development of biosensors. Among the most sensitive techniques available to study the biorecognition activity of the sensors, we highlight the biosensors based fluorescent, EIS, and QCM. The current systematic review focuses on the sensory diagnostic methods that are being used to detect HPV‐16 and 18 worldwide. Special emphasis is given on the sensory techniques that can diagnosis the individuals with CC. © 2018 BioFactors, 45(2):101–117, 2019

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“…The hybridization between the HPV type16 target and DNA probe was investigated by SWV. The detection limit of HPV type 16 DNA was found at 2.3 nM with a linear range of 10–200 nM .…”

Section: Methodsmentioning

confidence: 97%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

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“…A The N‐terminus of the NA probe was negatively charged, modified with three glutamic acid residues, whilst the amino side chain of lysine residue on C‐terminal was attached with an anthraquinone‐labeled pyrrolidinyl peptide nucleic acid (AQ‐PNA) via 4‐(anthraquinone‐2‐oxy) butyric acid, to fix the AQ‐PNA probe on the surface of the G‐PANI. The method employed an inexpensive device and the electrochemical response of the AQ‐PNA probe before and after the addition of target DNA was measured (Teengam, Siangproh, Tuantranont, Henry et al, ).…”

Section: Immobilization Methods Of Na Probes Onto Paper Substratementioning

confidence: 99%

“…The PNA assay shows a linear correlation in the 10 nM–10 mM concentration range as well as a successful mismatch detection (Yildiz, Alagappan, & Liedberg, ). PNA can also be electrostatically to develop a novel paper‐based electrochemical DNA biosensor (Teengam, Siangproh, Tuantranont, Henry et al, ), with a larger detection range from 10 nM to 200 nM. PNA probe can be modified with three glutamic acid residues at the N‐terminus to incorporate the negative charge to improve stability.…”

Section: Nucleic Acid Probes: From Native Dna/rna To Artificial Xnamentioning

confidence: 99%

“…More important, upon recognition of targets, NA probes form characteristic higher‐order conformational changes through hydrogen bonding, electrostatic interactions, shape effect, aromatic ring and/or base pairs aggradation, which allow them to open up a vast repertoire of target recognition and signal transduction patterns. For instance, NA probes have recently been incorporated into paper‐based analytical platforms for detecting nearly all chemical and biological entities, such as metal ions (J. Chen, Zhou, & Wen, ), NAs (Restaino & White, ), microRNA (Kalogianni, Kalligosfyri, Kyriakou, & Christopoulos, ), amino acids (Kaspar, Dettmer, Gronwald, & Oefner, ), polypeptide (Cao et al, ), protein complex (Saha & Jana, ), polysaccharides (S. Wang et al, ), carbohydrate (Yang et al, ), small organic compounds (Conzuelo et al, ), virus particles (Teengam, Siangproh, Tuantranont, Henry et al, ), bacteria (Burlage & Tillmann, ), and organelles and surface markers of cells (Shah, Kaushik, Zhu, Zhang, & Li, ).…”

Section: Introductionmentioning

confidence: 99%

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Paper‐based point‐of‐care test with xeno nucleic acid probes

Xiao

Jin

et al. 2019

Biotech & Bioengineering

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Bridging the unmet need of efficient point-of-care testing (POCT) in biomedical engineering research and practice with the emerging development in artificial synthetic xeno nucleic acids (XNAs), this review summarized the recent development in paperbased POCT using XNAs as sensing probes. Alongside the signal transducing mode and immobilization methods of XNA probes, a detailed evaluation of probe performance was disclosed. With these new aspects, both researchers in synthetic chemistry / biomedical engineering and physicians in clinical practice could gain new insights in designing, manufacturing and choosing suitable reagents and techniques for POCT. K E Y W O R D S aptamer, chemical modification, paper-based analytical devices (PADs), point-of-care testing (POCT), xeno nucleic acids (XNAs)

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“…30 In another work that aimed to detect human papillomavirus in order to examine the primary stages of cervical cancer, an anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe (AQ-PNA) combined with graphene-polyaniline (G-PANI) was used to modify the electrode surface in the electrochemical biosensor, and together they aided in enhancing sensitivity, conductivity and electron transfer rates. 59 …”

Section: ·4 Electrodes Characterization: Fabrication and Surface Modmentioning

confidence: 99%

Trends in Paper-based Electrochemical Biosensors: From Design to Application

et al. 2018

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Electrochemical bio-sensing using paper-based detection systems is the main focus of this review. The different existing designs of 2-dimensional and 3-dimensional sensors, and fabrication techniques are discussed. This review highlights the effect of adopting different sensor designs, distinct fabrication techniques, as well as different modification methods, in order to produce reliable and reproducible reading. The use of various nanomaterials have been demonstrated in order to modify the surface of electrodes during fabrication to further enhance the signal for subsequent analysis. The reviewed sensors were classified into categories based on their applications, such as diagnostics, environmental and food testing. One of the major advantages of using paper-based electrochemical sensors is the potential for miniaturization, which only requires relatively small amount of samples, and the low cost for the purpose of mass production. Additionally, most of the devices reviewed were made to be portable, making them well-suited for on-site detection. Finally, paper-based detection is an ideal platform to fabricate cost-effective, user-friendly and sensitive electrochemical biosensors, with large capacity for customization depending on functional needs.

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Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus

Cited by 202 publications

References 50 publications

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Paper‐based point‐of‐care test with xeno nucleic acid probes

Trends in Paper-based Electrochemical Biosensors: From Design to Application

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