Electrochemical properties of 316L stainless steel with culturing L929 fibroblasts

Abstract

Potentiodynamic polarization and impedance tests were carried out on 316L stainless steel with culturing murine fibroblast L929 cells to elucidate the corrosion behaviour of 316L steel with L929 cells and to understand the electrochemical interface between 316L steel and cells, respectively. Potential step test was carried out on 316L steel with type I collagen coating and culturing L929 cells to compare the effects of collagen and L929 cells. The open-circuit potential of 316L steel slightly shifted in a negative manner and passive current density increased with cells, indicating a decrease in the protective ability of passive oxide film. The pitting potential decreased with cells, indicating a decrease in the pitting corrosion resistance. In addition, a decrease in diffusivity at the interface was indicated from the decrease in the cathodic current density and the increase in the diffusion resistance parameter in the impedance test. The anodic peak current in the potential step test decreased with cells and collagen. Consequently, the corrosion resistance of 316L steel decreases with L929 cells. In addition, collagen coating would provide an environment for anodic reaction similar to that with culturing cells.

Electrochemical study of Type 304 and 316L stainless steels in simulated body fluids and cell cultures

“…It is reported that cells generate the extracellular matrix, which primarily consists of collagen. Hiromoto et al [11,12] found that collagen coating produced the same effect as the L929 cells on Type 316L, i.e. increased anodic passive current density and lowered pitting potentials.…”

Engineering of bone fixation metal implants biointerface—Application of parylene C as versatile protective coating

“…The value of the total impedance for the uncoated steel sample was close to 10 5 -10 6 Ω cm 2 , in both cases. This level is typical for this kind of material [20]. When the steel substrate was covered by the silane-parylene coatings, the level of total impedance of analyzed system increased to 10 9 Ω cm 2 [21].…”

Fretting corrosion processes and wear mechanisms in medical implants

“…The free corrosion potential of 316L or 304 with cells is lower than the potential without cells in simulated body fl uid. Moreover, pitting corrosion resistance decreases in the presence of cells (Hiromoto and Hanawa, 2006;Tang et al, 2006). The 316L alloy is more corroded in an environment with cells.…”