Electrochemical quantification of levothyroxine at disposable screen-printed electrodes

David, Melinda; Şerban, Adrian; Enache, Teodor Adrian; Florescu, Monica

doi:10.1016/j.jelechem.2022.116240

Cited by 11 publications

(3 citation statements)

References 34 publications

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“…Heart rate, arrhythmias, and cardiac output are raised in hyperthyroidism and lowered in hypothyroidism. Thyroid abnormalities alter immune system functions and are linked to cancer proliferation, apoptosis, and invasiveness [183]. Hyperthyroidism or hypothyroidism can occur when there are abnormally high or low levels of thyroid hormones [184].…”

Section: Applications In Thyroxine Detectionmentioning

confidence: 99%

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Haroon,

Stine

2023

Coatings

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Hormones regulate several physiological processes in living organisms, and their detection requires accuracy and sensitivity. Recent advances in nanostructured electrodes for the electrochemical detection of hormones are described. Nanostructured electrodes’ high surface area, electrocatalytic activity, and sensitivity make them a strong hormone detection platform. This paper covers nanostructured electrode design and production using MOFs, zeolites, carbon nanotubes, metal nanoparticles, and 2D materials such as TMDs, Mxenes, graphene, and conducting polymers onto electrodes surfaces that have been used to confer distinct characteristics for the purpose of electrochemical hormone detection. The use of aptamers for hormone recognition is producing especially promising results, as is the use of carbon-based nanomaterials in composite electrodes. These materials are optimized for hormone detection, allowing trace-level quantification. Various electrochemical techniques such as SWV, CV, DPV, EIS, and amperometry are reviewed in depth for hormone detection, showing the ability for quick, selective, and quantitative evaluation. We also discuss hormone immobilization on nanostructured electrodes to improve detection stability and specificity. We focus on real-time monitoring and tailored healthcare with nanostructured electrode-based hormone detection in clinical diagnostics, wearable devices, and point-of-care testing. These nanostructured electrode-based assays are useful for endocrinology research and hormone-related disease diagnostics due to their sensitivity, selectivity, and repeatability. We conclude with nanotechnology–microfluidics integration and tiny portable hormone-detection devices. Nanostructured electrodes can improve hormone regulation and healthcare by facilitating early disease diagnosis and customized therapy.

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Section: Applications In Thyroxine Detectionmentioning

confidence: 99%

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Haroon,

Stine

2023

Coatings

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“…Several works report the study of T4′s electrochemical response by employing different types of sensing platforms that can help to improve simple and reliable detection tools to effectively monitor levels during treatments in a miniaturized way [ 11 , 12 ]. There is a specific focus on platforms that are grounded using carbon-based materials and screen-printed electrodes [ 13 , 14 , 15 ], as they are great candidates for the fabrication of miniaturized (bio)sensors with high sensitivity and ease of(bio)functionalization.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Inkjet-Printed Thyroxine Sensor Based on Customized Graphene and Tunned Cyclodextrins as the Preconcentration Element

Ortiz-Aguayo,

Paré,

Gabriel

et al. 2024

Nanomaterials

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The determination of thyroid hormones has practical clinical significance for the diagnosis of hyperthyroidism and hypothyroidism diseases. Considering this aspect, a wide range of analytical methods for the detection of analytes, including immunoassay, chemiluminescence, mass spectroscopy and high-performance liquid chromatography, among others, has been developed. This type of analysis provides feasible results. Nevertheless, it requires qualified staff, special facilities and is time-consuming. For this reason, this paper relies on the fabrication of an electrochemical device developed with inkjet printing technology for the free detection of Thyroxine (T4). To manufacture our electrochemical device, several aspects were considered from the use of materials that amplify electrical signals, to finding a supramolecular scaffold that possess affinity towards the target analyte and the need of preconcentrating the analyte on the electrode’s surface. For this task, printed devices were modified with a hybrid nanomaterial consisting of reduced graphene oxide (rGO) tuned with Au nanoparticles (Au–NPs) and an entrapment agent and different thiolated cyclodextrins (x–CD-SH) as carrying agents. Analytes were preconcentrated via supramolecular chemistry due to the formation of an inclusion complex between the cyclodextrin and hormones. Morphological and electrochemical characterization of the final device was carried out to ensure the proper workability of the electrode, achieving excellent response, sensitivity and limit of detection (LOD).

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“…Literature can be found that reports on thyroid hormones (a small molecule type) in human or animal serum, urine, or tissues, either in its pure or synthetic form, by using sensors or methods that are the outcome of combining more than two techniques and at least one kind of antibody, all of them with the aim of contributing to the understanding of hypo-and hyperthyroidism. In that sense, a list of techniques includes chromatography coupled with mass spectroscopy [10][11][12][13][14][15], immunoassays with magnetic or plasmonic nanoparticles [16][17][18], electrochemical or chemiluminescence techniques complemented with immunoassays and/or nanoparticles [19][20][21][22][23][24], among others. Some of those techniques require pretreatment, purification, or labeling of the sample, while others use high-pressure conditions, which generate an increase in the analysis costs [10,12,13,16].…”

Section: Introductionmentioning

confidence: 99%

Thyroxine Quantification by Using Plasmonic Nanoparticles as SERS Substrates

De Leon Portilla,

González,

Sanchez-Mora

2023

Chemosensors

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Functionalized Au and Ag nanoparticles (NPs) with ascorbic and tannic acid, respectively, were used as SERS substrates (SS). Several SS were fabricated with different loads of metal NPs deposited on silicon wafers. We focused on the thyroxine (T4) band at 1044 cm−1 and tracked its intensity and position at concentrations from 10 pM to 1 mM. For all SS, the band intensity decreased as the T4 concentration decreased. Additionally, the band shifted to larger wavenumbers as the NP loads increased. In the case of Au, the SS with the highest load of NPs, the minimum concentration detected was 1 μM. The same load of the Ag NP SS showed a better performance detecting a concentration of 10 pM, an outcome from a SERS-EF of 109. The NP spatial distribution includes mainly isolated NPs, quasi-spherical clusters, and semi-linear arrays of NPs in random orientations. From the numerical simulations, we conclude that the hot spots at the interparticle gaps in a linear array of three NPs are the most intense. The Ag NP SS demonstrated good sensitivity, to allow the detection of pM concentrations. Therefore, its complementation to any immunoassay technique provides an interesting alternative for point-of-care implementations, such as test strips.

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Electrochemical quantification of levothyroxine at disposable screen-printed electrodes

Cited by 11 publications

References 34 publications

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Amperometric Inkjet-Printed Thyroxine Sensor Based on Customized Graphene and Tunned Cyclodextrins as the Preconcentration Element

Thyroxine Quantification by Using Plasmonic Nanoparticles as SERS Substrates

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