Electrochemical Real-Time Analysis of Bacterial Biofilm Adhesion and Development by Means of Thin-Film Biosensors

Becerro, S.; Paredes, Jacobo; Mujika, Maite; Lorenzo, Eva Perez; Arana, Sergio

doi:10.1109/jsen.2015.2504495

Cited by 14 publications

(4 citation statements)

References 46 publications

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“…Electrochemical methods allow for the observation of this phenomenon, their analysis, and subsequent detection of bacterial existence at the early phases of adhesive and biofilm development. Beccero et al, 2016 created a thin-film sensor [ 41 ]. It was developed by differential pulse voltammetry (DPV) and cyclic voltammetry (CV).…”

Section: Sensing Of Biofilmsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy-Based Sensing of Biofilms: A Comprehensive Review

Ameer,

Ibrahim,

Yaseen

et al. 2023

Biosensors

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Biofilms are complex communities of microorganisms that can form on various surfaces, including medical devices, industrial equipment, and natural environments. The presence of biofilms can lead to a range of problems, including infections, reduced efficiency and failure of equipment, biofouling or spoilage, and environmental damage. As a result, there is a growing need for tools to measure and monitor levels of biofilms in various biomedical, pharmaceutical, and food processing settings. In recent years, electrochemical impedance sensing has emerged as a promising approach for real-time, non-destructive, and rapid monitoring of biofilms. This article sheds light on electrochemical sensing for measuring biofilms, including its high sensitivity, non-destructive nature, versatility, low cost, and real-time monitoring capabilities. We also discussed some electrochemical sensing applications for studying biofilms in medical, environmental, and industrial settings. This article also presents future perspectives for research that would lead to the creation of reliable, quick, easy-to-use biosensors mounted on unmanned aerial vehicles (UAVs), and unmanned ground vehicles (UGVs), utilizing artificial intelligence-based terminologies to detect biofilms.

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Section: Sensing Of Biofilmsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy-Based Sensing of Biofilms: A Comprehensive Review

Ameer,

Ibrahim,

Yaseen

et al. 2023

Biosensors

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“…Reproduced with permission from Ref. [ 122 ] (k) Optical micrograph of the electrochemical camera chip with integrated electrodes and amplifiers highlighted. Chip is 1 cm x 1 cm.…”

Section: Microsystems For Biofilm Studiesmentioning

confidence: 99%

“…achieved this by developing a thin-film sensor ( Fig. 4 j) that was designed for cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements [ 122 ]. The authors use a four-microelectrode configuration, which comprises of two gold working electrodes along with a platinum counter electrode and a platinum pseudo-reference electrode.…”

Section: Microsystems For Biofilm Studiesmentioning

confidence: 99%

Microsystems for biofilm characterization and sensing – A review

Subramanian

Huiszoon

Chu

et al. 2020

Biofilm

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“…Research in the field of biosensors has made available a variety of technologies for the detection of minute amounts of biological samples, including biofilms. − Optical methods for studying biofilms have been used for macroscopic and microscopic studies. , However, a major disadvantage of optical sensors is the difficult integration and packaging of the biosensor components into a small footprint for simple use with medical implants. A method to address the concerns associated with three-dimensional optical systems is using a surface acoustic wave (SAW) sensor that enables sensitive real-time detection of biofilm formation. , The change in resonant frequency of the sensor gives a direct measure of bacterial loading on the surface.…”

Section: Introductionmentioning

confidence: 99%

An Integrated Microsystem for Real-Time Detection and Threshold-Activated Treatment of Bacterial Biofilms

Subramanian

Tolstaya

Winkler

et al. 2017

ACS Appl. Mater. Interfaces

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Bacterial biofilms are the primary cause of infections in medical implants and catheters. Delayed detection of biofilm infections contributes to the widespread use of high doses of antibiotics, leading to the emergence of antibiotic-resistant bacterial strains. Accordingly, there is an urgent need for systems that can rapidly detect and treat biofilm infections in situ. As a step toward this goal, in this work we have developed for the first time a threshold-activated feedback-based impedance sensor-treatment system for combined real-time detection and treatment of biofilms. Specifically, we demonstrate the use of impedimetric sensing to accurately monitor the growth of Escherichia coli biofilms in microfluidic flow cells by measuring the fractional relative change (FRC) in absolute impedance. Furthermore, we demonstrate the use of growth measurements as a threshold-activated trigger mechanism to initiate successful treatment of biofilms using bioelectric effect (BE), applied through the same sensing electrode array. This was made possible through a custom program that (a) monitored the growth and removal of biofilms within the microfluidic channels in real-time and (b) enabled the threshold-based activation of BE treatment. Such BE treatment resulted in a ∼74.8 % reduction in average biofilm surface coverage as compared to the untreated negative control. We believe that this smart microsystem for integrated biofilm sensing and treatment will enable future development of autonomous biosensors optimized for accurate real-time detection of the onset of biofilms and their in situ treatment, directly on the surfaces of medical implants.

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Electrochemical Real-Time Analysis of Bacterial Biofilm Adhesion and Development by Means of Thin-Film Biosensors

Cited by 14 publications

References 46 publications

Electrochemical Impedance Spectroscopy-Based Sensing of Biofilms: A Comprehensive Review

Electrochemical Impedance Spectroscopy-Based Sensing of Biofilms: A Comprehensive Review

Microsystems for biofilm characterization and sensing – A review

An Integrated Microsystem for Real-Time Detection and Threshold-Activated Treatment of Bacterial Biofilms

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