Electrochemical Reduction of Ellman's Reagent: A Novel Selective Detection Protocol for Thiol Compounds

Scampicchio, Matteo; Lawrence, Nathan S.; Arecchi, Alessandra; Mannino, Saverio

doi:10.1002/elan.200703970

Cited by 15 publications

(6 citation statements)

References 51 publications

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“…Electrochemical detection methods can offer a number of advantages, such as being fast, facile and easy to perform. The literature reports the use of electrochemical methods for thiol detection, but unfortunately not many offer selectivity without the use of extensive sample pre-treatments [21–34], such as separation techniques [15,25] , or the addition of reagents to increase selectivity and/or to avoid other thiols from interfering in the measurement [32,35,36]. Currently, there are only limited literature reports on the selective detection of specific thiols (homocysteine, glutathione, and/or cysteine) in the presence of each other using voltammetric methods [32,37,38].…”

Section: Introductionmentioning

confidence: 99%

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

Lee

Lowinsohn

Compton

2014

Sensors

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Screen printed electrodes were employed in a proof of concept determination of homocysteine and glutathione using electrochemically oxidized catechol via a 1,4-Michael addition reaction in the absence and presence of cysteine, and each other. Using cyclic voltammetry, the Michael reaction introduces a new adduct peak which is analytically useful in detecting thiols. The proposed procedure relies on the different rates of reaction of glutathione and homocysteine with oxidized catechol so that at fast voltage scan rates only homocysteine is detected in cyclic voltammetry. At slower scan rates, both glutathione and homocysteine are detected. The combination of the two sets of data provides quantification for homocysteine and glutathione. The presence of cysteine is shown not to interfere provided sufficient high concentrations of catechol are used. Calibration curves were determined for each homocysteine and glutathione detection; where the sensitivities are 0.019 μA·μM−1 and 0.0019 μA·μM−1 and limit of detections are ca. 1.2 μM and 0.11 μM for homocysteine and glutathione, respectively, within the linear range. This work presents results with potential and beneficial use in re-useable and/or disposable point-of-use sensors for biological and medical applications.

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Section: Introductionmentioning

confidence: 99%

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

Lee

Lowinsohn

Compton

2014

Sensors

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“…DTNBA, also called Ellman’s reagent, is one of the most widely used colorimetric agents for the detection of thiols in biological samples − . It can react with 2 equiv.…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Trace Cr³⁺ in Aqueous Solution by Using 5,5′-Dithiobis (2-Nitrobenzoic acid)-Modified Gold Nanoparticles

Dang

Wang

et al. 2009

ACS Appl. Mater. Interfaces

136

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A simply prepared gold nanoparticle-based sensor, 5,5'-dithiobis (2-nitrobenzoic acid) (DTNBA)-modified gold nanoparticles, was prepared to explore the sensitive and selective detection of metal ions using a colorimetric technique. The selective detection of trace levels (93.6 ppb) Cr3+ in aqueous solution was achieved over 15 other metal ions. The functionalized gold nanoparticles became aggregated in solution in the presence of Cr3+ by an ion-templated chelation process, which caused an easily measurable change in the extinction spectrum of the particles and provided an inherently sensitive method for Cr3+ detection in aqueous solution.

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“…Although Ellman’s reagent has been known in the literature since 1959, its redox activity was reported in 1999, after 40 years of its discovery . Few efforts were made toward the electrochemical quantification of free thiol concentration using the same . Recently, gold nanoparticles modified with Ellman’s reagent were reported for the selective optical sensing of biothiols .…”

Section: Resultsmentioning

confidence: 99%

“…32 Few efforts were made toward the electrochemical quantification of free thiol concentration using the same. 54 Recently, gold nanoparticles modified with Ellman's reagent were reported for the selective optical sensing of biothiols. 55 However, no attempts have been made to explore the anion sensing potential of Ellman's reagent yet.…”

Section: Resultsmentioning

confidence: 99%

Supersensitive Detection of Anions in Pure Organic and Aqueous Media by Amino Acid Conjugated Ellman’s Reagent

Dhawan

Devnani

Babu

et al. 2021

ACS Appl. Bio Mater.

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The last few decades witnessed a remarkable advancement in the field of molecular anion receptors. A variety of anion binding motifs have been discovered, and large number of designer molecular anion receptors with high selectivity are being reported. However, anion detection in an aqueous medium is still a formidable challenge as evident from only a miniscule of synthetic systems available in the literature. We, herein, report 5,5′-dithio-bis(2-nitrobenzoic acid) (Ellman’s reagent) appended with amino acids as supersensitive anion sensors that can detect F– and H2PO4 – ions in both aqueous as well as organic media. Interestingly, the sensors showed a dual response to anions, viz., chromogenic response in organic medium and electrochemical response in aqueous solutions. Various spectroscopic techniques such as UV–vis and 1H NMR are used to investigate the binding studies in acetonitrile, whereas electrochemical methods such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) are employed to explore the anion binding in water. The host–guest complex stoichiometry and binding constants are calculated using the BindFit software. The geometry of host–guest complex has been optimized by the density functional theory (DFT) method. These molecules are versatile sensors since these function in both water and acetonitrile with extremely low limit of detection (LOD) up to 0.07 fM and limit of quantification (LOQ) up to 0.23 fM. To our knowledge, the present system is the first example of a sensor that can detect the lowest concentration of anions in water quantitatively. The minimalistic design strategy presented here opens up the innumerable possibilities for designing dual anion sensors in a one fell swoop.

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Electrochemical Reduction of Ellman's Reagent: A Novel Selective Detection Protocol for Thiol Compounds

Cited by 15 publications

References 51 publications

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

Selective Detection of Trace Cr³⁺ in Aqueous Solution by Using 5,5′-Dithiobis (2-Nitrobenzoic acid)-Modified Gold Nanoparticles

Supersensitive Detection of Anions in Pure Organic and Aqueous Media by Amino Acid Conjugated Ellman’s Reagent

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Electrochemical Reduction of Ellman's Reagent: A Novel Selective Detection Protocol for Thiol Compounds

Cited by 15 publications

References 51 publications

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

The Use of Screen-Printed Electrodes in a Proof of Concept Electrochemical Estimation of Homocysteine and Glutathione in the Presence of Cysteine Using Catechol

Selective Detection of Trace Cr3+ in Aqueous Solution by Using 5,5′-Dithiobis (2-Nitrobenzoic acid)-Modified Gold Nanoparticles

Supersensitive Detection of Anions in Pure Organic and Aqueous Media by Amino Acid Conjugated Ellman’s Reagent

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Selective Detection of Trace Cr³⁺ in Aqueous Solution by Using 5,5′-Dithiobis (2-Nitrobenzoic acid)-Modified Gold Nanoparticles